Key features and details
- Rabbit polyclonal to HECTD3
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-HECTD3 antibody
See all HECTD3 primary antibodies
DescriptionRabbit polyclonal to HECTD3
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Mouse, Human
Synthetic peptide within Human HECTD3 aa 811-861. The exact sequence is proprietary.
Database link: Q5T447
- WB: HeLa, HEK-293T, Jurkat, TCMK-1 and NIH/3T3 whole cell lysates. IP: HEK-293T whole cell lysate.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab241413 was affinity purified using an epitope specific to HECTD3 immobilized on solid support.
Our Abpromise guarantee covers the use of ab241413 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/2500. Predicted molecular weight: 97 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionE3 ubiquitin ligases accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Mediates ubiquitination of TRIOBP and its subsequent proteasomal degradation, thus faciliting cell cycle progression by regulating the turn-over of TRIOBP. Mediates also ubiquitination of STX8.
PathwayProtein modification; protein ubiquitination.
Sequence similaritiesContains 1 DOC domain.
Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.
Cellular localizationCytoplasm > perinuclear region.
- Information by UniProt
- E3 ubiquitin-protein ligase HECTD3 antibody
- HECD3_HUMAN antibody
- HECT antibody
All lanes : Anti-HECTD3 antibody (ab241413) at 1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : TCMK-1 (mouse kidney epithelial cell line) whole cell lysate
Lane 5 : NIH/3T3 (mouse embyro fibroblast cell line) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 97 kDa
Exposure time: 30 seconds
Lysates prepared using NETN lysis buffer.
HECTD3 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab241413 at 6 µg/reaction. Western blot was performed from the immunoprecipitate using ab241413 at 1 µg/ml.
Lane 1: ab241413 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.
Detection: Chemiluminescence with exposure time of 3 minutes.
Lysates prepared using NETN lysis buffer.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab241413 has not yet been referenced specifically in any publications.