Overview

  • Product name
    Anti-HECTD3 antibody - C-terminal
    See all HECTD3 primary antibodies
  • Description
    Rabbit polyclonal to HECTD3 - C-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human HECTD3 aa 739-769 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: Q5T447

  • Positive control
    • WB: A549 cell lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab173122 was purified through a protein A column, followed by peptide affinity purification.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab173122 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 97 kDa.

Target

  • Function
    E3 ubiquitin ligases accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Mediates ubiquitination of TRIOBP and its subsequent proteasomal degradation, thus faciliting cell cycle progression by regulating the turn-over of TRIOBP. Mediates also ubiquitination of STX8.
  • Pathway
    Protein modification; protein ubiquitination.
  • Sequence similarities
    Contains 1 DOC domain.
    Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.
  • Cellular localization
    Cytoplasm > perinuclear region.
  • Information by UniProt
  • Database links
  • Alternative names
    • E3 ubiquitin-protein ligase HECTD3 antibody
    • HECD3_HUMAN antibody
    • HECT antibody
    • HECT domain containing protein 3 antibody
    • HECT domain-containing protein 3 antibody
    • HECTD3 antibody
    • Probable E3 ubiquitin protein ligase HECTD3 antibody
    see all

Images

  • Anti-HECTD3 antibody - C-terminal (ab173122) at 1/100 dilution + A549 cell line lysate at 35 µg

    Predicted band size: 97 kDa

References

This product has been referenced in:
  • Li Z  et al. HECTD3 Mediates an HSP90-Dependent Degradation Pathway for Protein Kinase Clients. Cell Rep 19:2515-2528 (2017). WB ; Human . Read more (PubMed: 28636940) »
See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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