Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-HEF1/NEDD-9 antibody [2G9]

Inconclusive
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Mouse brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid pH6
Permeabilization
No
Specification
Mouse brain
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: rt°C
Fixative
Formaldehyde

Other product details

Incubation time
2 hour(s) and 0 minute(s) · Diluent: TBS/BA/azide
Dilution
1/200

Secondary antibody

Dilution
1/200
Name
Non-Abcam antibody was used: anti rabbit Igs
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin

Additional data

Additional Notes
With this Ab, on mouse sections, I never see any nuclear positivity. I do not see any other positivity that could be interpreted as specific. However, I apologise for the lines in the section: known as "chatter" to us Histogeeks = poor cutting quality! This artefact causes regular , alternating, thick and thin lines of the section: these thick areas can non-specifically trap reagents!
Abcam response
Thank you for your honest any thoughtful feedback. While Abcam has not tested this antibody in IHC-P, your data suggest it may be unsuitable for this application.

Mr. Carl Hobbs

Verified customer

Submitted Jan 05 2009

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