Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-HEF1 / NEDD-9 antibody [2G9]

Inconclusive
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid pH6
Permeabilization
No
Specification
Brain
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: rt°C
Fixative
Formaldehyde

Other product details

Incubation time
2 hour(s) and 0 minute(s) · Diluent: TBS/BSA/azide
Dilution
1/200

Secondary antibody

Dilution
1/200
Name
Non-Abcam antibody was used: anti mouse Igs
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin

Additional data

Additional Notes
On paraffin wax sections, on the three species that I have tested with this Ab reagent, I think that this Ab is not appropriate for paraffin wax sections. The submitted image show, in my opinion, non-specific immunostaining. Rat brain: frontal cortex, showing corpus callosum to the right.
Abcam response
Thank you for your honest and thoughtful feedback. Although Abcam has yet to test this antibody in IHC-P, your data suggest it may be unsuitable for this application.

Mr. Carl Hobbs

Verified customer

Submitted Jan 05 2009

Sign up