Western blot abreview for Anti-HEF1/NEDD-9 antibody [2G9]

Good
Abreviews
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Neural stem cell line)
Gel Running Conditions
Reduced Denaturing (7.5%)
Loading amount
15 µg
Specification
Neural stem cell line
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Other product details

Incubation time
1 hour(s) and 0 minute(s)
Dilution
1/2000

Secondary antibody

Dilution
1/3000
Name
Non-Abcam antibody was used: Peroxidase labeled anti-mouse IgG (H+L)
Host species: Horse
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL
Exposure
1 hour(s), 0 minute(s) and 0 second(s)
Bands
Specific: 105 kDa Non-specific: 115 kDa

Ms. Melina Reisenberg

Verified customer

Submitted Jun 19 2009

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