Western blot abreview for Anti-HEF1/NEDD-9 antibody [2G9]

Excellent
Abreviews
Application
Western blot
Sample
Mouse Cell lysate - whole cell (embryonic stem)
Gel Running Conditions
Reduced Denaturing
Loading amount
15 µg
Specification
embryonic stem
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% BSA in TBS with 0.1% Tween
Dilution
1/2000

Secondary antibody

Dilution
1/20000
Name
Non-Abcam antibody was used: goat anti-mouse HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
Pierce ECL
Exposure
5 second(s)
Bands
Specific: doublet at 110 kDa

Additional data

Additional Notes
used phosphatase inhibitors in cell lysis buffer lanes 1-2: whole cell lysate from mouse embryonic stem cells

Prof. Reiter Lab

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Submitted Apr 20 2010

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