Western blot abreview for Anti-HEF1/NEDD-9 antibody [2G9]

Excellent
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (Breast cancer cell lines MDA-MB-231, HCC1954 and M)
Gel Running Conditions
Reduced Denaturing (8% acrylamide gel)
Loading amount
20 µg
Treatment
TGF beta 2.5ng/ml
Specification
Breast cancer cell lines MDA-MB-231, HCC1954 and M
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Other product details

Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Blocking buffer
Dilution
1/1000

Secondary antibody

Dilution
1/2000
Name
Non-Abcam antibody was used: DAKO Goat anti-mouse IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL
Exposure
20 second(s)
Bands
Specific: 115, 105 kDa

Wendy Greenwood

Verified customer

Submitted Jan 16 2015

Sign up