Immunoprecipitation abreview for Anti-HEF1/NEDD-9 antibody [2G9]

Good
Abreviews
Application
Immunoprecipitation
Sample
Human Cell lysate - other (Human Lung Adenocarcinoma A549 cell-line)
Total protein in input
1000 µg
Treatment
100ng/ml either HGF or EGF for 5 or 60 min
Immuno-precipitation step
Protein G
Specification
Human Lung Adenocarcinoma A549 cell-line

Other product details

Incubation time
20 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 0.5% NP-40 25mM tris (pH 7.5) 100mM NaCl
Dilution
1/1000

Western Blot antibody

Dilution
1/1000
Name
Non-Abcam antibody was used: anti Phosphotyrosine
Host species: Mouse
Clonality: Monoclonal
Conjugation: PE/Cy5.18 ®

Dr. Dean Hammond

Verified customer

Submitted Jun 07 2007

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