Western blot abreview for Anti-HEF1 antibody [2G9]

Excellent
Abreviews
Application
Western blot
Sample
Human Cell lysate - other (A549 Lung Carcinoma Cell Line)
Loading amount
20 µg
Specification
A549 Lung Carcinoma Cell Line
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%

Other product details

Dilution
1/2000
Incubation time
15 hour(s) and 0 minute(s)

Secondary antibody

Name
Non-Abcam antibody was used: Donkey anti-mouse IRDye800CW
Host species: Donkey
Clonality: Polyclonal
Conjugation: IRDye® 800CW

Detection

Detection method
Licor Odyssey IR Imager
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 105 kDa Non-specific: 55, 60 kDa
Positive control
none
Negative control
none

Additional data

Additional Notes
Lysis buffer used = 0.5% NP-40, 25mM Tris (pH 7.5), 100mM NaCl, with 1:250 Sigma mammalian protease inhibitors.
Transferred to 0.45 micron pore-size nitrocellulose membrane. 5x 5 min washes after primary antibody incubation.

Dr. Dean Hammond

Verified customer

Submitted May 24 2007

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