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Human Cell lysate - other (A549 Lung Carcinoma Cell Line)
A549 Lung Carcinoma Cell Line
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Other product details
15 hour(s) and 0 minute(s)
Non-Abcam antibody was used: Donkey anti-mouse IRDye800CW
Host species: Donkey
Conjugation: IRDye® 800CW
Licor Odyssey IR Imager
5 minute(s) and 0 second(s)
Specific: 105 kDa Non-specific: 55, 60 kDa
Lysis buffer used = 0.5% NP-40, 25mM Tris (pH 7.5), 100mM NaCl, with 1:250 Sigma mammalian protease inhibitors.
Transferred to 0.45 micron pore-size nitrocellulose membrane. 5x 5 min washes after primary antibody incubation.
Dr. Dean Hammond
Submitted May 24 2007