HeLa Apoptosis Lysate Set: Staurosporine-Treated and Vehicle-Treated Control (ab136806)

Overview

  • Product name

    HeLa Apoptosis Lysate Set: Staurosporine-Treated and Vehicle-Treated Control
  • Product overview

    Staurosporine is a broad-spectrum inhibitor of serine/threonine kinases and tyrosine kinases. It is commonly used to induce apoptosis in experimental settings, although the mechanism is not fully understood.
    The staurosporine-treated HeLa lysate is designed for use as a western blot positive control for antibodies against apoptosis-specific proteins or for experimental treatments to induce apoptosis.

  • Notes

    Confluent HeLa cells were treated with either the vehicle (DMSO, 0.2%) or staurosporine (1 uM) for 4 hours. Cells were pelleted then lysed with Lysis Buffer (50 mM Tris-HCl pH 7.5, 150 mM NaCl, 10 mM NaF, 1x Protease Inhibitors, 1% SDS) and the lysate cleared by sonication. The protein concentration was measured by BCA assay. Modified 5x SDS sample buffer was added to the lysate before storage at -80ºC. The lysates were validated by western blotting with the ab136812 Apoptosis WB cocktail (cleaved PARP, actin, caspase 3).

  • Tested applications

    Suitable for: WB, SDS-PAGEmore details

Properties

Applications

Our Abpromise guarantee covers the use of ab136806 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration.

Suggested working concentration: 2.0 mg/mL, Load 20 µg/lane.

User may choose to boil the sample before loading, at their discretion.

SDS-PAGE Use at an assay dependent concentration.

Suggested working concentration: 2.0 mg/mL, Load 20µg/lane.

User may choose to boil the sample before loading, at their discretion.

Images

  • Lane 1: HeLa, vehicle (DMSO) treated for 4 hours Lane 2: HeLa 1 µM staurpsorine, 4 hours Load 20 µg/lane 5% milk/PBST for block and antibody diluent Primary antibodies (2 hours, room temp) All lanes: ab136812 250X Primary Antibodies Cocktail, 1/250 dilution Secondary antibodies (1 hour, room temp) All lanes: ab136812 100X HRP-Conjugated Secondary Antibodies Cocktail, 1/100 dilution

References

ab136806 has not yet been referenced specifically in any publications.

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