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using Hela, U2OS and RPE1 cells - flow cytometry
nocodazole treatment to arrest in G2/m phase and then release
sees only for Hela cells a shoulder (subG1 phase) after release
staining DNA with PI and a kinase using an Ab
Asked on Oct 11 2012
Thank you for your phone call.
Could you please send me representative images/histograms showing the signal in question?
With this information I will be able to ask the lab and see if they have an explanation. Thank you.
I look forward to hear back from you.
Answered on Oct 11 2012