Question (63529) | HeLa Cell Cycle Lysates: Thymidine-Treated, Nocodazole-Treated & Untreated Asynchronous Control (ab136811)

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Question

using Hela, U2OS and RPE1 cells - flow cytometry
nocodazole treatment to arrest in G2/m phase and then release
sees only for Hela cells a shoulder (subG1 phase) after release
staining DNA with PI and a kinase using an Ab

Answer

Thank you for your phone call.

Could you please send me representative images/histograms showing the signal in question?

With this information I will be able to ask the lab and see if they have an explanation. Thank you.

I look forward to hear back from you.

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