HeLa Histone Acetylation-Inhibited Whole Cell Lysate Set: Trichostatin A-Treated and Vehicle-Treated (ab139414)
Key features and details
- Sample type: Cell Lysate
Overview
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Product name
HeLa Histone Acetylation-Inhibited Whole Cell Lysate Set: Trichostatin A-Treated and Vehicle-Treated -
Sample type
Cell Lysate -
Product overview
Trichostatin A-treated HeLa lysate serve as a positive controls for western blotting with antibodies to acetylated proteins. Trichostatin A is an inhibitor of class I and II histone deacetylases. A vehicle-treated HeLa lysate is also be included.
Concentration: HeLa trichostatin A-treated lysate, 200 µg at 2.0 mg/mL
HeLa vehicle-treated lysate, 200 µg at 2.0 mg/mL -
Notes
Acetylation of histone proteins is an important means of regulating gene transcription. Trichostatin A is an inhibitor of class I and II histone deacetylases, and can also function as a cell cycle inhibitor. The trichostatin A-treated HeLa lysate is designed for use as a western blot positive control for antibodies against acetylated histones or other proteins (e.g. p53). It can also be used as a control for experimental treatments to investigate acetylation or deacetylation of histones and other proteins.
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Tested applications
Suitable for: WB, SDS-PAGEmore details
Properties
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Storage instructions
Store at -80°C. Please refer to protocols. -
Components Identifier 2 units Trichostatin A-Treated HeLa Lysate — Control for Trichostatin A-Treated HeLa Lysate 2 units -
Research areas
Associated products
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Related Products
Applications
Our Abpromise guarantee covers the use of ab139414 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use at an assay dependent concentration. | |
| SDS-PAGE | Use at an assay dependent concentration. |
Images
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WB analysis of HeLa lysates using an anti- acetyl lysine antibody.
Lane 1: HeLa, vehicle-treated lysate
Lane 2: HeLa, trichostatin A-treated lysate
All lysates at 20 µg/lane
Primary antibody
All lanes: ab21623 anti-acetyl Lysine antibody
Secondary antibody
All lanes: HRP-conjugated Goat anti-Rabbit, 1:5000 dilution
Developed using ECL technique -
WB analysis of HeLa lysates using anti-Histone H3 acetyl lysine antibodies.Lanes 1, 3: HeLa, vehicle-treated lysate
Lanes 2, 4: HeLa, trichostatin A-treated lysate
All lysates at 20 µg/lane
Primary antibodies
Lanes 1, 2: ab52946 anti-Histone H3 acetyl lysine 14
Lanes 3, 4: ab47915 anti-Histone H3 acetyl lysine 9, 14, 18, 23, 27
Secondary antibody
All lanes: HRP-conjugated Goat anti-Rabbit, 1:5000 dilution
Developed using ECL technique
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (0)
ab139414 has not yet been referenced specifically in any publications.
