HeLa Histone Acetylation-Inhibited Whole Cell Lysate Set: Trichostatin A-Treated and Vehicle-Treated (ab139414)

Overview

  • Product name
    HeLa Histone Acetylation-Inhibited Whole Cell Lysate Set: Trichostatin A-Treated and Vehicle-Treated
  • Sample type
    Cell Lysate
  • Product overview

    Trichostatin A-treated HeLa lysate serve as a positive controls for western blotting with antibodies to acetylated proteins. Trichostatin A is an inhibitor of class I and II histone deacetylases. A vehicle-treated HeLa lysate is also be included.

     

    Concentration: HeLa trichostatin A-treated lysate, 200 µg at 2.0 mg/mL

                               HeLa vehicle-treated lysate, 200 µg at 2.0 mg/mL

  • Notes

    Acetylation of histone proteins is an important means of regulating gene transcription. Trichostatin A is an inhibitor of class I and II histone deacetylases, and can also function as a cell cycle inhibitor. The trichostatin A-treated HeLa lysate is designed for use as a western blot positive control for antibodies against acetylated histones or other proteins (e.g. p53). It can also be used as a control for experimental treatments to investigate acetylation or deacetylation of histones and other proteins.

  • Tested applications
    Suitable for: WB, SDS-PAGEmore details

Properties

Applications

Our Abpromise guarantee covers the use of ab139414 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration.
SDS-PAGE Use at an assay dependent concentration.

Images

  • Lane 1: HeLa, vehicle-treated lysate
    Lane 2: HeLa, trichostatin A-treated lysate

    All lysates at 20 µg/lane

    Primary antibody
    All lanes: ab21623 anti- acetyl lysine

    Secondary antibody
    All lanes: HRP-conjugated Goat anti-Rabbit, 1:5000 dilution
    Developed using ECL technique
  • Lanes 1, 3: HeLa, vehicle-treated lysate
    Lanes 2, 4: HeLa, trichostatin A-treated lysate

    All lysates at 20 µg/lane

    Primary antibodies
    Lanes 1, 2: ab52946 anti-Histone H3 acetyl lysine 14
    Lanes 3, 4: ab47915 anti-Histone H3 acetyl lysine 9, 14, 18, 23, 27

    Secondary antibody
    All lanes: HRP-conjugated Goat anti-Rabbit, 1:5000 dilution
    Developed using ECL technique

References

ab139414 has not yet been referenced specifically in any publications.

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