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Kits/ Lysates/ Other Lysates Whole Cell Lysates Human HeLa
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HeLa Histone Acetylation-Inhibited Whole Cell Lysate Set: Trichostatin A-Treated and Vehicle-Treated (ab139414)

  • Datasheet
  • SDS
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WB analysis of HeLa lysates using an anti- acetyl lysine antibody.
  • WB analysis of HeLa lysates using anti-Histone H3 acetyl lysine antibodies.

Key features and details

  • Sample type: Cell Lysate

Overview

  • Product name

    HeLa Histone Acetylation-Inhibited Whole Cell Lysate Set: Trichostatin A-Treated and Vehicle-Treated
  • Sample type

    Cell Lysate
  • Product overview

    Trichostatin A-treated HeLa lysate serve as a positive controls for western blotting with antibodies to acetylated proteins. Trichostatin A is an inhibitor of class I and II histone deacetylases. A vehicle-treated HeLa lysate is also be included.


    Concentration: HeLa trichostatin A-treated lysate, 200 µg at 2.0 mg/mL
                               HeLa vehicle-treated lysate, 200 µg at 2.0 mg/mL

  • Notes

    Acetylation of histone proteins is an important means of regulating gene transcription. Trichostatin A is an inhibitor of class I and II histone deacetylases, and can also function as a cell cycle inhibitor. The trichostatin A-treated HeLa lysate is designed for use as a western blot positive control for antibodies against acetylated histones or other proteins (e.g. p53). It can also be used as a control for experimental treatments to investigate acetylation or deacetylation of histones and other proteins.

  • Tested applications

    Suitable for: WB, SDS-PAGEmore details

Properties

  • Storage instructions

    Store at -80°C. Please refer to protocols.
  • Components Identifier 2 units
    Trichostatin A-Treated HeLa Lysate —
    Control for Trichostatin A-Treated HeLa Lysate 2 units
  • Research areas

    • Kits/ Lysates/ Other
    • Lysates
    • Whole Cell Lysates
    • Human
    • HeLa

Associated products

  • Related Products

    • Anti-acetyl Lysine antibody - ChIP Grade (ab21623)
    • Anti-Histone H3 (acetyl K9 + K14 + K18 + K23 + K27) antibody (ab47915)
    • Anti-Histone H3 (acetyl K14) antibody [EP964Y] - ChIP Grade (ab52946)

Applications

Our Abpromise guarantee covers the use of ab139414 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration.
SDS-PAGE Use at an assay dependent concentration.

Images

  • WB analysis of HeLa lysates using an anti- acetyl lysine antibody.
    WB analysis of HeLa lysates using an anti- acetyl lysine antibody.

    Lane 1: HeLa, vehicle-treated lysate
    Lane 2: HeLa, trichostatin A-treated lysate

    All lysates at 20 µg/lane

    Primary antibody
    All lanes: ab21623 anti-acetyl Lysine antibody

    Secondary antibody
    All lanes: HRP-conjugated Goat anti-Rabbit, 1:5000 dilution
    Developed using ECL technique

  • WB analysis of HeLa lysates using anti-Histone H3 acetyl lysine antibodies.
    WB analysis of HeLa lysates using anti-Histone H3 acetyl lysine antibodies.
    Lanes 1, 3: HeLa, vehicle-treated lysate
    Lanes 2, 4: HeLa, trichostatin A-treated lysate

    All lysates at 20 µg/lane

    Primary antibodies
    Lanes 1, 2: ab52946 anti-Histone H3 acetyl lysine 14
    Lanes 3, 4: ab47915 anti-Histone H3 acetyl lysine 9, 14, 18, 23, 27

    Secondary antibody
    All lanes: HRP-conjugated Goat anti-Rabbit, 1:5000 dilution
    Developed using ECL technique

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

    • Datasheet
    • SDS
  • References (0)

    Publishing research using ab139414? Please let us know so that we can cite the reference in this datasheet.

    ab139414 has not yet been referenced specifically in any publications.

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