HeLa Histone Acetylation-Inhibited Whole Cell Lysate Set: Trichostatin A-Treated and Vehicle-Treated (ab139414)
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Overview
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Product name
HeLa Histone Acetylation-Inhibited Whole Cell Lysate Set: Trichostatin A-Treated and Vehicle-Treated -
Sample type
Cell Lysate -
Product overview
Trichostatin A-treated HeLa lysate serve as a positive controls for western blotting with antibodies to acetylated proteins. Trichostatin A is an inhibitor of class I and II histone deacetylases. A vehicle-treated HeLa lysate is also be included.
Concentration: HeLa trichostatin A-treated lysate, 200 µg at 2.0 mg/mL
HeLa vehicle-treated lysate, 200 µg at 2.0 mg/mL -
Notes
Acetylation of histone proteins is an important means of regulating gene transcription. Trichostatin A is an inhibitor of class I and II histone deacetylases, and can also function as a cell cycle inhibitor. The trichostatin A-treated HeLa lysate is designed for use as a western blot positive control for antibodies against acetylated histones or other proteins (e.g. p53). It can also be used as a control for experimental treatments to investigate acetylation or deacetylation of histones and other proteins.
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Tested applications
Suitable for: WB, SDS-PAGEmore details
Properties
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Storage instructions
Store at -80°C. Please refer to protocols. -
Components Identifier 2 units Trichostatin A-Treated HeLa Lysate — Control for Trichostatin A-Treated HeLa Lysate 2 units -
Research areas
Associated products
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Related Products
Applications
Our Abpromise guarantee covers the use of ab139414 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use at an assay dependent concentration. | |
| SDS-PAGE | Use at an assay dependent concentration. |
Images
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WB analysis of HeLa lysates using an anti- acetyl lysine antibody.Lane 1: HeLa, vehicle-treated lysate
Lane 2: HeLa, trichostatin A-treated lysate
All lysates at 20 µg/lane
Primary antibody
All lanes: ab21623 anti- acetyl lysine
Secondary antibody
All lanes: HRP-conjugated Goat anti-Rabbit, 1:5000 dilution
Developed using ECL technique -
WB analysis of HeLa lysates using anti-Histone H3 acetyl lysine antibodies.Lanes 1, 3: HeLa, vehicle-treated lysate
Lanes 2, 4: HeLa, trichostatin A-treated lysate
All lysates at 20 µg/lane
Primary antibodies
Lanes 1, 2: ab52946 anti-Histone H3 acetyl lysine 14
Lanes 3, 4: ab47915 anti-Histone H3 acetyl lysine 9, 14, 18, 23, 27
Secondary antibody
All lanes: HRP-conjugated Goat anti-Rabbit, 1:5000 dilution
Developed using ECL technique
References
ab139414 has not yet been referenced specifically in any publications.
