HeLa nuclear extract lysate (TNF alpha stimulated) (ab14652)

Overview

  • Product name
    HeLa nuclear extract lysate (TNF alpha stimulated)
    See all HeLa lysates
  • Description
    HeLa nuclear extract lysate
  • General notes
    The HeLa nuclear extracts have been collected in Lysis Buffer after a 30 minute incubation with or without TNF alpha. The protein content has been determined by a Bradford based assay (20 ng/ml). We recommend aliquoting the extracts into single use fractions and then storing.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.90
    Constituents: 0.48% HEPES, 2.05% Sodium chloride, 20% Glycerol, 0.01% Magnesium chloride, 0.015% EDTA, 0.004% EGTA, 0.077% DTT, 1% NP-40
    Note: With Protease inhibitors
  • Concentration information loading...
  • Research areas
  • Background
    HeLa cells are human epithelial cells from a fatal cervical carcinoma. The cell line was derived from cervical cancer cells taken from Henrietta Lacks, in 1951. Horizontal gene transfer from human papillomavirus 18 (HPV18) to human cervical cells created the HeLa genome which is different from either parent genome in various ways including its number of chromosomes. HeLa cells have a modal chromosome number of 82, with 4 copies of chromosome 12 and 3 copies of chromosomes 6, 8, and 17. HeLa cells are adherent cells (they stick to surfaces) and maintain contact inhibition in vitro.

Images

  • Each lot has been tested for NFkappaB activation. The signal intensity for NFkappaB activation in each lot is compared to the signal intensity obtained with extracts from unstimulated HeLa cells. After the signals are blanked, the ratio of the signals from stimulated cells over unstimulated cells must be above 4. This ratio may vary depending on the basal level of NFkappaB activation in a given cell type.

References

ab14652 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-3 of 3 Q&A

Answer

Thank you for contacting us regarding ab14652HeLa (Human epithelial carcinoma cell line) Nuclear Lysate - TNF alpha stimulated,

I want to apologize for the delay in sending this information to you. The cells in this lysate are not starved before the treatment.

The Lysis Buffer consists of 20 mM Hepes pH 7.5, 350 mM NaCl, 20% glycerol, 1% Igepal-CA630, 1 mM MgCl2, 0.5 mM EDTA and 0.1 mM EGTA, 5 mM DTT and protease inhibitors.



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Question
Answer

Thank you for your enquiry. Yes, this lysate can be used in EMSA. If you have any additional questions, please contact us again.

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Answer

Thank you for your enquiry. The HeLa nuclear extracts have been collected in Lysis Buffer after a 30-minute incubation with TNF-a (20 ng/ml). The Lysis Buffer consists of 20 mM Hepes pH 7.5, 350 mM NaCl, 20% glycerol, 1% Igepal-CA630, 1 mM MgCl2, 0.5 mM EDTA and 0.1 mM EGTA, 5 mM DTT and protease inhibitors. The protein content has been determined by a Bradford-based assay. Please do note hesitate to contact us if you need further information,

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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