HeLa nuclear extract lysate (TNF alpha stimulated) (ab14652)

Q&A (3)

Overview

  • Product name

    HeLa nuclear extract lysate (TNF alpha stimulated)
    See all HeLa lysates

  • General notes

    The HeLa nuclear extracts have been collected in Lysis Buffer after a 30 minute incubation with or without TNF alpha. The protein content has been determined by a Bradford based assay (20 ng/ml). We recommend aliquoting the extracts into single use fractions and then storing.

Properties

  • Mycoplasma free

    Yes

  • Form

    Liquid

  • Storage instructions

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

  • Storage buffer

    pH: 7.90
    Constituents: 0.004% EGTA, 0.01% Magnesium chloride, 0.077% DTT, 0.48% HEPES, 0.015% EDTA, 20% Glycerol, 2.05% Sodium chloride, 1% Igepal

    With Protease inhibitors
  • Concentration information loading...

  • Research areas

  • Background

    HeLa cells are human epithelial cells from a fatal cervical carcinoma. The cell line was derived from cervical cancer cells taken from Henrietta Lacks, in 1951. Horizontal gene transfer from human papillomavirus 18 (HPV18) to human cervical cells created the HeLa genome which is different from either parent genome in various ways including its number of chromosomes. HeLa cells have a modal chromosome number of 82, with 4 copies of chromosome 12 and 3 copies of chromosomes 6, 8, and 17. HeLa cells are adherent cells (they stick to surfaces) and maintain contact inhibition in vitro.

Images

  • Functional Studies - HeLa nuclear extract lysate (TNF alpha stimulated) (ab14652)
    Functional Studies - HeLa nuclear extract lysate (TNF alpha stimulated) (ab14652)

    Each lot has been tested for NFkappaB activation. The signal intensity for NFkappaB activation in each lot is compared to the signal intensity obtained with extracts from unstimulated HeLa cells. After the signals are blanked, the ratio of the signals from stimulated cells over unstimulated cells must be above 4. This ratio may vary depending on the basal level of NFkappaB activation in a given cell type.

References

ab14652 has not yet been referenced specifically in any publications.

Publishing research using ab14652? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

Q&A
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1-3 of 3 Q&A

Question

Hello,   I had a couple of questions regarding your TNFa treated HeLa nuclear extract lysate, ab14652.  Are the cells starved overnight before treatment?  If so, how long?  What type of lysis buffer is used?

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Answer

Thank you for contacting us regarding ab14652HeLa (Human epithelial carcinoma cell line) Nuclear Lysate - TNF alpha stimulated,

I want to apologize for the delay in sending this information to you. The cells in this lysate are not starved before the treatment.

The Lysis Buffer consists of 20 mM Hepes pH 7.5, 350 mM NaCl, 20% glycerol, 1% Igepal-CA630, 1 mM MgCl2, 0.5 mM EDTA and 0.1 mM EGTA, 5 mM DTT and protease inhibitors.



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Question

Can this lysate be used in EMSA?

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Answer

Thank you for your enquiry. Yes, this lysate can be used in EMSA. If you have any additional questions, please contact us again.

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Question

This quiery is regarding TNF-alpha stimulated Hela Cell Lysates: How long the cells have been stimulated with TNF alpha and at what concentration??

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Answer

Thank you for your enquiry. The HeLa nuclear extracts have been collected in Lysis Buffer after a 30-minute incubation with TNF-a (20 ng/ml). The Lysis Buffer consists of 20 mM Hepes pH 7.5, 350 mM NaCl, 20% glycerol, 1% Igepal-CA630, 1 mM MgCl2, 0.5 mM EDTA and 0.1 mM EGTA, 5 mM DTT and protease inhibitors. The protein content has been determined by a Bradford-based assay. Please do note hesitate to contact us if you need further information,

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