HeLa nuclear extract lysate (TNF alpha stimulated) (ab14652)

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Functional Studies - HeLa nuclear extract lysate (TNF alpha stimulated) (ab14652)

    Overview

    • Product name

      HeLa nuclear extract lysate (TNF alpha stimulated)
      See all HeLa lysates

    • General notes

      The HeLa nuclear extracts have been collected in Lysis Buffer after a 30 minute incubation with or without TNF alpha. The protein content has been determined by a Bradford based assay (20 ng/ml). We recommend aliquoting the extracts into single use fractions and then storing.

      Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
      It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

    Properties

    • Mycoplasma free

      Yes

    • Form

      Liquid

    • Storage instructions

      Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    • Storage buffer

      pH: 7.5
      Constituents: 0.004% EGTA, 0.01% Magnesium chloride, 0.077% DTT, 0.48% HEPES, 0.015% EDTA, 20% Glycerol, 2.05% Sodium chloride, 1% Ethoxy Ethanol

      With Protease inhibitors
    • Concentration information loading...

    • Research areas

    • Background

      HeLa cells are human epithelial cells from a fatal cervical carcinoma. The cell line was derived from cervical cancer cells taken from Henrietta Lacks, in 1951. Horizontal gene transfer from human papillomavirus 18 (HPV18) to human cervical cells created the HeLa genome which is different from either parent genome in various ways including its number of chromosomes. HeLa cells have a modal chromosome number of 82, with 4 copies of chromosome 12 and 3 copies of chromosomes 6, 8, and 17. HeLa cells are adherent cells (they stick to surfaces) and maintain contact inhibition in vitro.

    Images

    • Functional Studies - HeLa nuclear extract lysate (TNF alpha stimulated) (ab14652)
      Functional Studies - HeLa nuclear extract lysate (TNF alpha stimulated) (ab14652)

      Each lot has been tested for NFkappaB activation. The signal intensity for NFkappaB activation in each lot is compared to the signal intensity obtained with extracts from unstimulated HeLa cells. After the signals are blanked, the ratio of the signals from stimulated cells over unstimulated cells must be above 4. This ratio may vary depending on the basal level of NFkappaB activation in a given cell type.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab14652? Please let us know so that we can cite the reference in this datasheet.

    ab14652 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    1-3 of 3 Abreviews or Q&A

    Question

    Hello,   I had a couple of questions regarding your TNFa treated HeLa nuclear extract lysate, ab14652.  Are the cells starved overnight before treatment?  If so, how long?  What type of lysis buffer is used?

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    Answer

    Thank you for contacting us regarding ab14652HeLa (Human epithelial carcinoma cell line) Nuclear Lysate - TNF alpha stimulated,

    I want to apologize for the delay in sending this information to you. The cells in this lysate are not starved before the treatment.

    The Lysis Buffer consists of 20 mM Hepes pH 7.5, 350 mM NaCl, 20% glycerol, 1% Igepal-CA630, 1 mM MgCl2, 0.5 mM EDTA and 0.1 mM EGTA, 5 mM DTT and protease inhibitors.



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    I hope that this information is helpful. Please let me know if you have any questions or there are other ways that Abcam may help you meet your research goals.

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    Question

    Can this lysate be used in EMSA?

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    Answer

    Thank you for your enquiry. Yes, this lysate can be used in EMSA. If you have any additional questions, please contact us again.

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    Question

    This quiery is regarding TNF-alpha stimulated Hela Cell Lysates: How long the cells have been stimulated with TNF alpha and at what concentration??

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    Answer

    Thank you for your enquiry. The HeLa nuclear extracts have been collected in Lysis Buffer after a 30-minute incubation with TNF-a (20 ng/ml). The Lysis Buffer consists of 20 mM Hepes pH 7.5, 350 mM NaCl, 20% glycerol, 1% Igepal-CA630, 1 mM MgCl2, 0.5 mM EDTA and 0.1 mM EGTA, 5 mM DTT and protease inhibitors. The protein content has been determined by a Bradford-based assay. Please do note hesitate to contact us if you need further information,

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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