Product nameHeLa nuclear extract lysate (TPA stimulated 2 hr)
See all HeLa lysates
DescriptionHeLa nuclear extract lysate
General notesCells were cultured in medium supplemented with 50 ng/mL TPA (phorbol, 12 myristate, 13 acetate) for two hours at 37°C immediately prior to harvesting. We recommend aliquoting the extracts into single use fractions and then storing.
Tested applicationsSuitable for: WB, EMSAmore details
Storage instructionsShipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
Storage bufferPreservative: None
Constituents: 20% Glycerol, 20mM HEPES, 100mM Potassium Chloride, 1mM Magnesium Chloride, 0.5mM PMSF, 0.5mM DTT. pH 7.9
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BackgroundHeLa cells are human epithelial cells from a fatal cervical carcinoma. The cell line was derived from cervical cancer cells taken from Henrietta Lacks, in 1951. Horizontal gene transfer from human papillomavirus 18 (HPV18) to human cervical cells created the HeLa genome which is different from either parent genome in various ways including its number of chromosomes. HeLa cells have a modal chromosome number of 82, with 4 copies of chromosome 12 and 3 copies of chromosomes 6, 8, and 17. HeLa cells are adherent cells (they stick to surfaces) and maintain contact inhibition in vitro.
Our Abpromise guarantee covers the use of ab14653 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent dilution.|
|EMSA||Use at an assay dependent dilution.|
ab14653 has not yet been referenced specifically in any publications.