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Kits/ Lysates/ Other Lysates Whole Cell Lysates Human HeLa
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HeLa Signaling Cascade Whole Cell Lysate Set: PMA-Treated and Vehicle-Treated Control (ab170195)

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Western Blot Validation
  • Optiblot Blue stain

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Preparation
Product image
HeLa Whole Cell RIPA Extract (ab170197)

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Overview

  • Product name

    HeLa Signaling Cascade Whole Cell Lysate Set: PMA-Treated and Vehicle-Treated Control
  • Product overview

    HeLa cells are an adherent human epithelial cell line derived from a cervical carcinoma. HeLa cells were lysed with 1% SDS in the prescence of protease and phosphatase inhibitors. Protein concentration of the extract was determined by BCA assay. The extract is suitable for use in SDS-PAGE and Western blotting.


    PMA-treated HeLa lysate is designed for use as a western blot positive control when studying signaling cascades downstream of PKC. Cells were treated with 200 nM PMA for 1 hour after overnight serum starvation. Untreated cells were grown under the same conditions and treated instead with DMSO (PMA diluent as used to generate the treated lysate).


    Concentration: HeLa PMA-treated lysate, 200 µg at 2.0 mg/mL
                                HeLa DMSO-treated lysate, 200 µg at 2.0 mg/mL

  • Notes

    PMA (phorbol 12-myristate 13-acetate) also known as TPA (12-O-Tetradecanoylphorbol 13-acetate), is a potent activator of protein kinase C (PKC), a calcium-activated, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase. PKC is involved in positive and negative regulation of cell proliferation, apoptosis, differentiation, migration and adhesion, tumorigenesis, cardiac hypertrophy, angiogenesis, platelet function and inflammation, by directly phosphorylating targets such as RAF1, BCL2, CSPG4, TNNT2/CTNT, or activating signaling cascade involving MAPK1/3 (ERK1/2) and RAP1GAP.

     

  • Tested applications

    Suitable for: SDS-PAGE, WBmore details

Properties

  • Storage instructions

    Store at -80°C. Please refer to protocols.
  • Components 2 units
    Control for PMA-Treated HeLa Lysate 1 x 200µg
    PMA-Treated HeLa Lysate 1 x 200µg
  • Research areas

    • Kits/ Lysates/ Other
    • Lysates
    • Whole Cell Lysates
    • Human
    • HeLa

Associated products

  • Related Products

    • Anti-RSK1 p90 antibody [E4] (ab32114)
    • Anti-RSK1 p90 (phospho S380) antibody [E239] (ab32203)
    • Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab8226)

Applications

Our Abpromise guarantee covers the use of ab170195 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
SDS-PAGE Use at an assay dependent concentration.

Load 20 µg – 40 µg per lane

Heat sample at 70°C before loading

WB Use at an assay dependent concentration.

Load 20 µg – 40 µg per lane

Heat sample at 70°C before loading

Images

  • Western Blot Validation
    Western Blot Validation

    HeLa cell cultures were serum-starved overnight and treated with 200nM of PMA for 1 hour at 37°C. 40µg of cell lysate was run on a 4-20% gradient gel then transferred to a PVDF membrane. All blocking and antibody incubation steps were done in 5% milk, 20 mM Tris-HCl, 0.1% TWEEN-20. Primary antibodies: RSK1p90 total: 1:1000 ab32114 RSK1p90 pSer380: 1:500 ab32203 Actin beta: 1µg/mL ab8226 Secondary antibodies: Goat polyclonal to Mouse IgG – H&L – Pre-Adsorbed (HRP) at 1/10000. Goat polyclonal to Rabbit IgG – H&L – Pre-Adsorbed (HRP) at 1/10000.

  • Optiblot Blue stain
    Optiblot Blue stain

    Optiblot Blue stain  HeLa cell cultures were serum-starved overnight and treated with 200 nM of PMA for 1 hour at 37°C. 40 µg of cell lysate was run on a 4-20% gradient gel then stained with Optiblot Blue (ab119211) for 2 hours at room temperature. Gel was destained using nanopure water for 24 hours.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

    • Datasheet
    • SDS
  • References (0)

    Publishing research using ab170195? Please let us know so that we can cite the reference in this datasheet.

    ab170195 has not yet been referenced specifically in any publications.

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