HeLa whole cell lysate (ab150035)
- Datasheet
- References
- Protocols
Overview
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Product name
HeLa whole cell lysate
See all HeLa (Human epithelial carcinoma cell line) lysates -
General notes
We recommend aliquoting the extracts into single use fractions and then storing them at -80°C.This HeLa whole cell was prepared using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a non-reducing lithium dodecyl sulfate sample loading buffer (LDS).
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Tested applications
Suitable for: WBmore details
Properties
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Mycoplasma free
Yes -
Form
Liquid -
Storage instructions
Shipped on Dry Ice. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.50
Constituent: 10% DTT
This lysate was normalised with SDS-PAGE sample buffer to current concentration. -
Concentration information loading... -
Lysate notes
This HeLa whole cell was prepared using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a non-reducing lithium dodecyl sulfate sample loading buffer (LDS).
Applications
Our Abpromise guarantee covers the use of ab150035 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use at an assay dependent concentration. |
Images
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Western blot - HeLa whole cell lysate (ab150035)All lanes : Anti-Actin antibody - Loading Control (ab1801) at 1 µg/ml
Lane 1 :HeLa whole cell lysate (ab150035)
Lane 2 :HeLa nuclear extract lysate (ab150036)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Observed band size: 42 kDa why is the actual band size different from the predicted? -
Western blot - HeLa whole cell lysate (ab150035)All lanes : Anti-TATA binding protein TBP antibody - Nuclear Loading Control and ChIP Grade (ab63766) at 1 µg/ml
Lane 1 :HeLa whole cell lysate (ab150035)
Lane 2 :HeLa nuclear extract lysate (ab150036)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
Observed band size: 45 kDa why is the actual band size different from the predicted? -
SDS-PAGE - HeLa whole cell lysate (ab150035)All lanes stained with Optiblot Blue (ab119211) (20 ml)
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate (ab150035)
Lysates/proteins at 10 µg per lane. -
Western blot - HeLa whole cell lysate (ab150035)All lanes : Anti-beta Tubulin antibody (ab21057) at 1 µg/ml
Lane 1 :HeLa whole cell lysate (ab150035)
Lane 2 :HeLa nuclear extract lysate (ab150036)
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Rabbit polyclonal to Goat IgG - H&L - Pre-Adsorbed (HRP) (ab65486) at 1/3000 dilution
Observed band size: 51 kDa why is the actual band size different from the predicted? -
Western blot - HeLa whole cell lysate (ab150035)All lanes : Anti-HDAC1 antibody (ab19845) at 1 µg/ml
Lane 1 :HeLa whole cell lysate (ab150035)
Lane 2 :HeLa nuclear extract lysate (ab150036)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Observed band size: 60 kDa why is the actual band size different from the predicted?
References
ab150035 has not yet been referenced specifically in any publications.
