HeLa whole cell lysate (ab150035)

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Western blot - HeLa whole cell lysate (ab150035)
  • Western blot - HeLa whole cell lysate (ab150035)
  • SDS-PAGE - HeLa whole cell lysate (ab150035)
  • Western blot - HeLa whole cell lysate (ab150035)
  • Western blot - HeLa whole cell lysate (ab150035)

Overview

  • Product name

    HeLa whole cell lysate
    See all HeLa (Human epithelial carcinoma cell line) lysates

  • General notes

    We recommend aliquoting the extracts into single use fractions and then storing them at -80°C.

     

    This HeLa whole cell was prepared using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT).

  • Tested applications

    Suitable for: WBmore details

Properties

  • Mycoplasma free

    Yes

  • Form

    Liquid

  • Storage instructions

    Shipped on Dry Ice. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.50
    Constituent: 10% DTT
  • Concentration information loading...

  • Lysate notes

    This HeLa whole cell was prepared using a standard whole cell lysate protocol. 

Associated products

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab150035 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB
    Use at an assay dependent concentration.
    Notes
    WB
    Use at an assay dependent concentration.

    Images

    • Western blot - HeLa whole cell lysate (ab150035)
      Western blot - HeLa whole cell lysate (ab150035)
      All lanes : Anti-Actin antibody - Loading Control (ab1801) at 1 µg/ml

      Lane 1 : HeLa whole cell lysate (ab150035)
      Lane 2 : HeLa nuclear extract lysate (ab150036)

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

      Observed band size: 42 kDa
      why is the actual band size different from the predicted?

    • Western blot - HeLa whole cell lysate (ab150035)
      Western blot - HeLa whole cell lysate (ab150035)
      All lanes : Anti-TATA binding protein TBP antibody - Nuclear Loading Control and ChIP Grade (ab63766) at 1 µg/ml

      Lane 1 : HeLa whole cell lysate (ab150035)
      Lane 2 : HeLa nuclear extract lysate (ab150036)

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution

      Observed band size: 45 kDa why is the actual band size different from the predicted?

    • SDS-PAGE - HeLa whole cell lysate (ab150035)
      SDS-PAGE - HeLa whole cell lysate (ab150035)
      All lanes  stained with Optiblot Blue (ab119211) (20 ml)

      Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate (ab150035)

      Lysates/proteins at 10 µg per lane.
    • Western blot - HeLa whole cell lysate (ab150035)
      Western blot - HeLa whole cell lysate (ab150035)
      All lanes : Anti-beta Tubulin antibody (ab21057) at 1 µg/ml

      Lane 1 : HeLa whole cell lysate (ab150035)
      Lane 2 : HeLa nuclear extract lysate (ab150036)

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Rabbit polyclonal to Goat IgG - H&L - Pre-Adsorbed (HRP) (ab65486) at 1/3000 dilution

      Observed band size: 51 kDa why is the actual band size different from the predicted?

    • Western blot - HeLa whole cell lysate (ab150035)
      Western blot - HeLa whole cell lysate (ab150035)
      All lanes : Anti-HDAC1 antibody (ab19845) at 1 µg/ml

      Lane 1 : HeLa whole cell lysate (ab150035)
      Lane 2 : HeLa nuclear extract lysate (ab150036)

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

      Observed band size: 60 kDa why is the actual band size different from the predicted?

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab150035? Please let us know so that we can cite the reference in this datasheet.

    ab150035 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
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    Question

    Dear recipient,
    Does the Dkk1 antibody ab 61275 include the positive control mentioned
    in the data sheet (HeLa cell lysate)? Or should I purchase it
    separately?
    Thank you very much for your reply.
    Best regards,

    Read More
    Answer

    Thank you for contacting us.

    I can confirm that the positive control is not included into an order of the DKK antibody ab61275.

    However, we do offer two products of HeLa cell lysate: ab29545 (tested for WB application) and ab150035 that can be used as positive controls for WB. For further information about both products use the provided links below.

    ab29545
    https://www.abcam.com/HeLa-whole-cell-lysate-ab29545.html (or use the following: https://www.abcam.com/HeLa-whole-cell-lysate-ab29545.html).


    ab150035
    https://www.abcam.com/HeLa-whole-cell-lysate-ab150035.html (or use the following: https://www.abcam.com/HeLa-whole-cell-lysate-ab150035.html).


    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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