HeLa whole cell lysate (ab150035)

Q&A (1)

Overview

  • Product name
    HeLa whole cell lysate
  • General notes
    We recommend aliquoting the extracts into single use fractions and then storing them at -80°C.


    This HeLa whole cell was prepared using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a non-reducing lithium dodecyl sulfate sample loading buffer (LDS).

  • Tested applications
    Suitable for: WBmore details

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped on Dry Ice. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.50
    Constituent: 10% DTT
    Note: This lysate was normalised with SDS-PAGE sample buffer to current concentration.
  • Concentration information loading...
  • Lysate notes
    This HeLa whole cell was prepared using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a non-reducing lithium dodecyl sulfate sample loading buffer (LDS).

Applications

Our Abpromise guarantee covers the use of ab150035 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration.

Images

  • Western blot - HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate (ab150035)
    Western blot - HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate (ab150035)
    All lanes : Anti-Actin antibody - Loading Control (ab1801) at 1 µg/ml

    Lane 1 : HeLa whole cell lysate (ab150035)
    Lane 2 : HeLa nuclear extract lysate (ab150036)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Observed band size: 42 kDa
    why is the actual band size different from the predicted?

  • Western blot - HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate (ab150035)
    Western blot - HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate (ab150035)
    All lanes : Anti-TATA binding protein TBP antibody - Nuclear Loading Control and ChIP Grade (ab63766) at 1 µg/ml

    Lane 1 : HeLa whole cell lysate (ab150035)
    Lane 2 : HeLa nuclear extract lysate (ab150036)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution

    Observed band size: 45 kDa why is the actual band size different from the predicted?

  • SDS-PAGE - HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate (ab150035)
    SDS-PAGE - HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate (ab150035)
    All lanes  stained with Optiblot Blue (ab119211) (20 ml)

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate (ab150035)

    Lysates/proteins at 10 µg per lane.
  • Western blot - HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate (ab150035)
    Western blot - HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate (ab150035)
    All lanes : Anti-beta Tubulin antibody (ab21057) at 1 µg/ml

    Lane 1 : HeLa whole cell lysate (ab150035)
    Lane 2 : HeLa nuclear extract lysate (ab150036)

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Rabbit polyclonal to Goat IgG - H&L - Pre-Adsorbed (HRP) (ab65486) at 1/3000 dilution

    Observed band size: 51 kDa why is the actual band size different from the predicted?

  • Western blot - HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate (ab150035)
    Western blot - HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate (ab150035)
    All lanes : Anti-HDAC1 antibody (ab19845) at 1 µg/ml

    Lane 1 : HeLa whole cell lysate (ab150035)
    Lane 2 : HeLa nuclear extract lysate (ab150036)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Observed band size: 60 kDa why is the actual band size different from the predicted?

References

ab150035 has not yet been referenced specifically in any publications.

Publishing research using ab150035? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

Q&A
Submit a question

Question

Dear recipient,
Does the Dkk1 antibody ab 61275 include the positive control mentioned
in the data sheet (HeLa cell lysate)? Or should I purchase it
separately?
Thank you very much for your reply.
Best regards,

Read More
Answer

Thank you for contacting us.

I can confirm that the positive control is not included into an order of the DKK antibody ab61275.

However, we do offer two products of HeLa cell lysate: ab29545 (tested for WB application) and ab150035 that can be used as positive controls for WB. For further information about both products use the provided links below.

ab29545
https://www.abcam.com/index.html?datasheet=29545 (or use the following: https://www.abcam.com/index.html?datasheet=29545).


ab150035
https://www.abcam.com/index.html?datasheet=150035 (or use the following: https://www.abcam.com/index.html?datasheet=150035).


I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up