Overview

  • Product name
    HeLa whole cell lysate
    See all HeLa lysates
  • General notes


    Protein is isolated from HeLa cell pellet using a proprietary technique. The pellet was frozen in liquid nitrogen immediately and then stored at -70ºC. Quality control: 1. The isolated protein pattern on SDS-PAGE gel is visualized by coomassie blue staining. The pattern is consistent with each lot. 2. The isolated protein is Western Blot analyzed by either a GAPDH or beta-actin antibody. The expression level is consistent with each lot.

  • Tested applications
    Suitable for: WBmore details

Properties

  • Form
    Liquid
  • Storage instructions
    Store at -80°C. Allow to warm to room temp and agitate gently before aliquotting.
  • Storage buffer
    Constituents: 1% Tergitol-NP40, Potassium chloride, 0.1% Bromophenol blue, Magnesium chloride, 1.2% Beta mercaptoethanol, 2% SDS, HEPES, Sucrose, 10% Glycerol

    With Protease inhibitor cocktail/EDTA/Sodium deoxycholate
  • Concentration information loading...
  • Lysate notes
    Protein is isolated from HeLa cell pellet using a proprietary technique. The pellet was frozen in liquid nitrogen immediately and then stored at -70ºC. Quality control: 1. The isolated protein pattern on SDS-PAGE gel is visualized by coomassie blue staining. The pattern is consistent with each lot. 2. The isolated protein is Western Blot analyzed by either a GAPDH or beta-actin antibody. The expression level is consistent with each lot.
  • Research areas
  • Background
    HeLa cells are human epithelial cells from a fatal cervical carcinoma. The cell line was derived from cervical cancer cells taken from Henrietta Lacks, in 1951. Horizontal gene transfer from human papillomavirus 18 (HPV18) to human cervical cells created the HeLa genome which is different from either parent genome in various ways including its number of chromosomes. HeLa cells have a modal chromosome number of 82, with 4 copies of chromosome 12 and 3 copies of chromosomes 6, 8, and 17. HeLa cells are adherent cells (they stick to surfaces) and maintain contact inhibition in vitro.

Applications

Our Abpromise guarantee covers the use of ab29545 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution. Samples need to boiled for 5 minutes and put on ice before use.

References

ab29545 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-10 of 13 Q&A

Answer

Thank you for contacting us.

Yes, both ab85226 and ab134121 detect the same SLC7A5 (LAT1) protein. http://www.uniprot.org/uniprot/Q01650

We would guarantee ab134121 to work in WB, IP, and IHC-P with human samples. We know that it does not work well with Flow Cyt, ICC/IF and rat or mouse samples.

Cat # ab89545 is an EEA1 antibody no longer available on our catalogue. You must mean ab29545, the HeLa cell lysate. Yes, that would be a good positive control for use with ab134121.

In WB with ab134141, we recommend a working dilution of 1/1000 - 1/10000. The amount that you will need will depend on the amount present in your samples. The image generated on our website was done with a 1/1000 dilution for example.

A suitable secondary would be Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) to be used with ECL detection, like our ab133406.

https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Rabbit-IgG-HL-HRP-ab97051.html

https://www.abcam.com/optiblot-ecl-detect-kit-23pg-187ng-ab133406.html

You can find our full range of Optiblot WB reagents here: https://www.abcam.com/index.html?pageconfig=resource&rid=14709&source=pagetrap&viapagetrap=optiblot

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Answer

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number xxxxx. Included in this order are positive control lysates for these products. To check the status of the order please contact our Customer Service team and reference this number. Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know. I wish you the best of luck with your research.

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Answer

Thank you for contacting us.

I can confirm that the positive control is not included into an order of the DKK antibody ab61275.

However, we do offer two products of HeLa cell lysate: ab29545 (tested for WB application) and ab150035 that can be used as positive controls for WB. For further information about both products use the provided links below.

ab29545
https://www.abcam.com/index.html?datasheet=29545 (or use the following: https://www.abcam.com/index.html?datasheet=29545).


ab150035
https://www.abcam.com/index.html?datasheet=150035 (or use the following: https://www.abcam.com/index.html?datasheet=150035).


I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Answer

Thank you for contacting us.

We ship many products at 4C so it is not uncommon for this product to be arrived at room temperature. The product will be absolutely fine, please use it without any hesitation.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Question
Answer

Merci de nous avoir contactés.

Comme discuté au téléphone, nous allons vous envoyer un remplacement gratuit du lysat cellulaire HeLa ab29545. Jeme permets de vous transférer le numéro de commande de remplacement gratuit de ce produit :xx . Vous recevrez prochainement un mail de confirmation comprenant les détails d'expédition.

N'hésitez pas à nous contacter lors d'une prochaine occasion.

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Answer

Thank you for contacting us.

We do not sell cells, only cell lysates. I apologize but we will not be able to provide these in a pre-lysate form.

Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Answer

Thank you for contacting us.

We unfortunately do not sell cell lines.

The product ab29545 is a cell line lysates for use in western blot.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Answer

Thank you for contacting us.

I have arranged for the shipment of the Hela cell lysate ab29545 on order number xxx.
Please let me know what results you are obtaining and if this helps to interprete your results with the mouse samples.

I look forward to hear back from you and assist you further.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Question
Answer

Thank you for calling Abcam.

I have sent you a vial of ab29545, HeLa cell lysate to use as a positive control.

Please let me know if there is anything else I can help you with, or if continue to experience any issue with our antibodies.

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Answer

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab29545.

To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

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1-10 of 13 Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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