HeLa whole cell lysate (TNF alpha stimulated) (ab14658)

Overview

  • Product name

    HeLa whole cell lysate (TNF alpha stimulated)
    See all HeLa lysates
  • Description

    HeLa whole cell lysate
  • General notes

    The HeLa whole cell extracts were collected in Lysis Buffer after a 30 minute incubation with or without TNF alpha (20 ng/ml). The protein content was determined by a Bradford based assay. We recommend aliquoting the extracts into single use fractions and then storing them.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.50
    Constituents: 20% Glycerol, 2.05% Sodium chloride, 0.48% HEPES, 0.01% Magnesium chloride, 0.015% EDTA, 0.004% EGTA, 1% NP-40
  • Concentration information loading...
  • Research areas

  • Background

    HeLa cells are human epithelial cells from a fatal cervical carcinoma. The cell line was derived from cervical cancer cells taken from Henrietta Lacks, in 1951. Horizontal gene transfer from human papillomavirus 18 (HPV18) to human cervical cells created the HeLa genome which is different from either parent genome in various ways including its number of chromosomes. HeLa cells have a modal chromosome number of 82, with 4 copies of chromosome 12 and 3 copies of chromosomes 6, 8, and 17. HeLa cells are adherent cells (they stick to surfaces) and maintain contact inhibition in vitro.

Images

  • Ab14658 has been tested for NFkappaB activation. The signal intensity for NFkappaB activation is compared to the signal intensity obtained with extracts from unstimulated HeLa cells. After the signals are blanked, the ratio of the signals from stimulated cells over unstimulated cells must be above 4. This ratio may vary depending on the basal level of NFkappaB activation in a given cell type.

References

ab14658 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Q&A

Answer

Thank you for contacting us.

We do not sell cells, only cell lysates. I apologize but we will not be able to provide these in a pre-lysate form.

Please do not hesitate to contact us if you need any more advice or information.

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Question
Answer

Thank you for your enquiry. The extract works in ELISA and should work for detection of p65 also. I hope this information helps. Please do not hesitate to contact us if you need any more advice or information.

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