HeLa whole cell lysate (TNF alpha stimulated) (ab14658)

Q&A (2)

Overview

  • Product name
    HeLa whole cell lysate (TNF alpha stimulated)
    See all HeLa lysates
  • Description
    HeLa whole cell lysate
  • General notes
    The HeLa whole cell extracts were collected in Lysis Buffer after a 30 minute incubation with or without TNF alpha (20 ng/ml). The protein content was determined by a Bradford based assay. We recommend aliquoting the extracts into single use fractions and then storing them.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.50
    Constituents: 20% Glycerol, 2.05% Sodium chloride, 0.48% HEPES, 0.01% Magnesium chloride, 0.015% EDTA, 0.004% EGTA, 1% NP-40
  • Concentration information loading...
  • Purity
    Whole Cell Lysate
  • Research areas
  • Background
    HeLa cells are human epithelial cells from a fatal cervical carcinoma. The cell line was derived from cervical cancer cells taken from Henrietta Lacks, in 1951. Horizontal gene transfer from human papillomavirus 18 (HPV18) to human cervical cells created the HeLa genome which is different from either parent genome in various ways including its number of chromosomes. HeLa cells have a modal chromosome number of 82, with 4 copies of chromosome 12 and 3 copies of chromosomes 6, 8, and 17. HeLa cells are adherent cells (they stick to surfaces) and maintain contact inhibition in vitro.

Images

  • - HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate - TNF alpha stimulated (ab14658)
    - HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate - TNF alpha stimulated (ab14658)

    Ab14658 has been tested for NFkappaB activation. The signal intensity for NFkappaB activation is compared to the signal intensity obtained with extracts from unstimulated HeLa cells. After the signals are blanked, the ratio of the signals from stimulated cells over unstimulated cells must be above 4. This ratio may vary depending on the basal level of NFkappaB activation in a given cell type.

References

ab14658 has not yet been referenced specifically in any publications.

Publishing research using ab14658? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

Q&A
Submit a question

1-2 of 2 Q&A

Question

I have a question related to that previous order.

The lysates I bought are not ideal for my purpose, the PEG content is too high for LCMS analysis. And I am afraid of how much protein loss I will incur if trying to remove them from the sample. Would it be possible to get the same cells (regular, IL1 and TNF stimulated) but in a pre-lysate form (PBS washed pellets or lyophilized form) so that I could carry my own sample prep?

I would be interest in i.e. 10-100 million cells quantity.

Read More
Answer

Thank you for contacting us.

We do not sell cells, only cell lysates. I apologize but we will not be able to provide these in a pre-lysate form.

Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

Read More

Question

Work in ELISA, p65- needed as a positive cell line?

Read More
Answer

Thank you for your enquiry. The extract works in ELISA and should work for detection of p65 also. I hope this information helps. Please do not hesitate to contact us if you need any more advice or information.

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up