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    hela-whole-cell-lysate-tnf-alpha-stimulated-ab14658.pdf

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Kits/ Lysates/ Other Lysates Whole Cell Lysates Human Other
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HeLa whole cell lysate (TNF alpha stimulated) (ab14658)

  • Datasheet
  • SDS
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Functional Studies - HeLa whole cell lysate (TNF alpha stimulated) (ab14658)

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    Overview

    • Product name

      HeLa whole cell lysate (TNF alpha stimulated)
      See all HeLa lysates
    • General notes

      The HeLa whole cell extracts were collected in Lysis Buffer after a 30 minute incubation with or without TNF alpha (20 ng/ml). The protein content was determined by a Bradford based assay. We recommend aliquoting the extracts into single use fractions and then storing them.

      Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
      It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

    Properties

    • Mycoplasma free

      Yes
    • Form

      Liquid
    • Storage instructions

      Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
    • Storage buffer

      pH: 7.50
      Constituents: 0.004% EGTA, 0.01% Magnesium chloride, 0.48% HEPES, 0.015% EDTA, 20% Glycerol, 2.05% Sodium chloride, 1% Igepal
    • Concentration information loading...
    • Research areas

      • Kits/ Lysates/ Other
      • Lysates
      • Whole Cell Lysates
      • Human
      • Other
    • Background

      HeLa cells are human epithelial cells from a fatal cervical carcinoma. The cell line was derived from cervical cancer cells taken from Henrietta Lacks, in 1951. Horizontal gene transfer from human papillomavirus 18 (HPV18) to human cervical cells created the HeLa genome which is different from either parent genome in various ways including its number of chromosomes. HeLa cells have a modal chromosome number of 82, with 4 copies of chromosome 12 and 3 copies of chromosomes 6, 8, and 17. HeLa cells are adherent cells (they stick to surfaces) and maintain contact inhibition in vitro.

    Images

    • Functional Studies - HeLa whole cell lysate (TNF alpha stimulated) (ab14658)
      Functional Studies - HeLa whole cell lysate (TNF alpha stimulated) (ab14658)

      Ab14658 has been tested for NFkappaB activation. The signal intensity for NFkappaB activation is compared to the signal intensity obtained with extracts from unstimulated HeLa cells. After the signals are blanked, the ratio of the signals from stimulated cells over unstimulated cells must be above 4. This ratio may vary depending on the basal level of NFkappaB activation in a given cell type.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab14658? Please let us know so that we can cite the reference in this datasheet.

    ab14658 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    1-2 of 2 Abreviews or Q&A

    Question

    I have a question related to that previous order.

    The lysates I bought are not ideal for my purpose, the PEG content is too high for LCMS analysis. And I am afraid of how much protein loss I will incur if trying to remove them from the sample. Would it be possible to get the same cells (regular, IL1 and TNF stimulated) but in a pre-lysate form (PBS washed pellets or lyophilized form) so that I could carry my own sample prep?

    I would be interest in i.e. 10-100 million cells quantity.

    Read More

    Abcam community

    Verified customer

    Asked on Jul 26 2012

    Answer

    Thank you for contacting us.

    We do not sell cells, only cell lysates. I apologize but we will not be able to provide these in a pre-lysate form.

    Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Abcam Scientific Support

    Answered on Jul 26 2012

    Question

    Work in ELISA, p65- needed as a positive cell line?

    Read More

    Abcam community

    Verified customer

    Asked on Nov 30 2009

    Answer

    Thank you for your enquiry. The extract works in ELISA and should work for detection of p65 also. I hope this information helps. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Abcam Scientific Support

    Answered on Nov 30 2009

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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