Key features and details
- Rabbit polyclonal to HELB - C-terminal
- Suitable for: IP, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-HELB antibody - C-terminal
DescriptionRabbit polyclonal to HELB - C-terminal
Tested applicationsSuitable for: IP, WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide within Human HELB aa 1037-1087 (C terminal). The exact sequence is proprietary. NP_387467.2
Database link: Q8NG08
- HeLa, 293T, Jurkat, TCMK-1 and NIH3T3 whole cell lysates.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab202141 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-10 µg/mg of lysate.|
|WB||1/2000 - 1/10000. Predicted molecular weight: 123 kDa.|
FunctionUnwinds duplex DNA with 5'-3' polarity. Has single-strand DNA-dependent ATPase and DNA helicase activities. Prefers ATP and dATP as substrates. During S phase, may facilitate cellular recovery from replication stress.
Tissue specificityHighly expressed in testis and thymus and weakly in liver, spleen, kidney and brain.
Cellular localizationPreferentially during S phase, recruited to chromatin following DNA damage induced by UV irradiation, or camptothecin or hydroxyurea treatment.
- Information by UniProt
- DNA helicase B antibody
- hDHB antibody
- helB antibody
- HELB_HUMAN antibody
All lanes : Anti-HELB antibody - C-terminal (ab202141) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : 293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lane 4 : TCMK-1 whole cell lysate
Lane 5 : NIH3T3 whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 123 kDa
Exposure time: 30 seconds
Lane 1: Detection of HELB in immunoprecipitates of 293T whole cell lysate (prepared using NETN lysis buffer; 0.5 or 1.0 mg per IP reaction, 20% of IP loaded) using ab202141 at 6 µg per reaction, and at 0.4 µg/ml for subsequent Western blot detection.
Lane 2: Control IgG
Detection: Chemiluminescence with an exposure time of 30 seconds.
ab202141 has not yet been referenced specifically in any publications.