Overview

  • Product name
    Anti-Helicobacter pylori antibody
    See all Helicobacter pylori primary antibodies
  • Description
    Rabbit polyclonal to Helicobacter pylori
  • Host species
    Rabbit
  • Specificity
    Whole cell lysate. Has not been absorbed and may react with related microorganisms.
  • Tested applications
    Suitable for: ELISA, ICC/IF, Conjugation, IHC-Pmore details
  • Species reactivity
    Reacts with Helicobacter pylori. Not yet tested in other species.
  • Immunogen

    ATCC strain 43504

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.1% Sodium azide
    Constituent: 0.0268% PBS
  • Concentration information loading...
  • Purity
    Protein A purified
  • Purification notes
    >95% pure
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab20459 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/5000.
ICC/IF 1/1000.
Conjugation Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.

Target

  • Relevance
    The spiral shaped bacterium Helicobacter pylori is strongly associated with inflammation of the stomach and is also implicated in the development of gastric malignancy. H. pylori is known to cause peptic ulcers and chronic gastritis in human. It is associated with duodenal ulcers and may be involved in development of adenocarcimona and low-grade lymphoma of mucosa associated lymphoid tissue in the stomach. More recently this bacterium has also been implicated with a number of vascular disorders including heart disease. It is not clear how H. pylori is transmitted or why some patients become symptomatic while others do not. The bacteria are most likely spread from person to person through fecal-to-oral or oral-to-oral routes. Possible environmental reservoirs include contaminated water sources. Serological tests that measure specific H. pylori IgG antibodies can determine if a person has been infected although these methods do have drawbacks and limitations.
  • Alternative names
    • H. pylori antibody

References

This product has been referenced in:
  • Propheter DC  et al. Resistin-like molecule ß is a bactericidal protein that promotes spatial segregation of the microbiota and the colonic epithelium. Proc Natl Acad Sci U S A 114:11027-11033 (2017). Read more (PubMed: 28973871) »
  • Hoppe S  et al. Rapid identification of novel immunodominant proteins and characterization of a specific linear epitope of Campylobacter jejuni. PLoS One 8:e65837 (2013). Read more (PubMed: 23734261) »
See all 3 Publications for this product

Customer reviews and Q&As

Answer

Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

The details you have kindly provided will enable us to investigate this case for you and this is also helpful in our records for monitoring of quality. In the event that a product is not functioning in the applications cited on the product data sheet within our 6 month guarantee period, we will be pleased to provide a credit note or free of charge replacement.

Reviewing this case, I would like to offer some suggestions to help optimise the results fromthese antibodies.I would also appreciate if you can confirm some further details:

1. Please confirm the order number and date of purchase? I am sorry the last order from Belgium for this antibody was over 1 year ago.Also, Iam sorry to confirm that ab20459 Anti-Helicobacter pylori antibody jas not been tested in this type of cell based ELISA, it has been tested in indirect ELISA only.Regrettably, this would no longer be covered by the 6 month guarantee, or guaranteed for this application.

2. As the protocol uses cells coated on the plates and then incubated with the H. pylori organisms. The Tween in the buffers may be dissolving the cell membranes. It is also possible that the number of H. pylori organisms per well are too low for detection,manymay be washed away during the procedure.

3. In testing, the Helicobacter pylori isdirectly grownin the microtiter wells. A suspension of the organisms (barely hazy) is dispensed to the wells (50-100uL per well). The plates are air-dried at room temperature overnight or until dry. The plates should then be washed with a buffer containing Tween 20 to remove any loosely bound organisms before running the assay.

There is also a published reference from another researcher using this antibody in a nanoparticle assay. I have included a link to this paper. I hope you may find this helpful:

http://cvi.asm.org/cgi/reprint/12/3/418

I hope this information is helpful, thank you for your cooperation. Should the suggestions not improve the results, please do not hesitate to contact me again with the further requested details.

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up