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A standard curve is used to accurately determine the concentration of your sample from the signal generated by an assay. The signal is never perfectly proportional to the sample concentration. A standard curve is designed to correct for these effects, so you know which concentration a given signal value corresponds to.
Standard curves are generally used when you need quantitatively accurate results. The general principles described below apply to our cell-based, biochemical and protein activity assays, as well as our ELISA kits.
Specific instructions for your kit can be found in the protocol booklet's 'Data Analysis' section.
Specific instructions for standard curves will be indicated in the relevant protocol booklet under the ‘data analysis’ section