How should I measure samples from a microplate?
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Summary
Many kits will detect samples using a standard microplate format. Your plate choice will depend upon the detection method. Please see below for a summary of the plate types we recommend for each type of reading:
Colorimetric – clear plate
Fluorometric – black/clear-bottom plate
Luminometric – white plate
Opaque plates are required for some types of reading to eliminate signal cross-over between wells. We recommend to always use a flat bottom plate, and a plate with a clear bottom is absolutely necessary if using bottom-reading instruments.
Always remember to remove plate sealers & plate lids before taking the measurement. These will not only interfere with the microplate reading, but pose a high risk of damaging the instrument
What are the benefits of top-reading v bottom reading?
Top-reading often provides a better signal-to-noise ratio. This reading can eliminate cross-talk between wells and reduce background fluorescence.
Bottom reading, however, often provides better results when working with cell-based assays. This is because some assay components (e.g. oily buffers) can interfere with the excitation or emission of the fluorophore, making it impossible to read from the top of the plate.