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See below for a summary of the different IHC/ICC applications that we list on our datasheets, and what they refer to.
Application | Summary | Sample prep | Detection |
Immunocytochemistry | Single cell layer deposited onto slide or plate
Fixation: immersion - generally in PFA or methanol
| Direct or indirect colorimetric / fluorometric detection | |
IHC-P | Immunohistochemistry (paraffin-embedded) |
Sample embedded in paraffin prior to sectioning
| Direct or indirect colorimetric / fluorometric detection |
IHC-Fr | Immunohistochemistry (frozen sections) | Direct or indirect colorimetric / fluorometric detection | |
IHC-FoFr | Immunohistochemistry (perfusion fixed frozen sections) | Direct or indirect colorimetric / fluorometric detection | |
IHC-FrFl | Immunohistochemistry (free-floating frozen sections) | Fixation: Immersion and perfusion
Sample is frozen prior to sectioning
Immunostaining takes place with the sample floating in solution.
| Direct or indirect colorimetric / fluorometric detection |
Immunohistochemistry (whole-mount staining) | Fixation: immersion Tissue is not sectioned; immunostaining takes place on small pieces of tissue, usually embryos. Similar to ICC, but sample is generally much larger. | Direct or indirect colorimetric / fluorometric detection |
Note: there can be overlap between the different applications that we list on our datasheets.
For example: