NLRP3 target tips
NLRP3 (NACHT, LRR, and PYD domains-containing protein 3) is a protein encoded by the NLRP3 gene. NLRP3 is expressed predominantly in macrophages and plays a crucial role in innate immunity and inflammation.
Protein function, expression, and isoforms
Protein function
- NLRP3 may function as an inducer of apoptosis.
- It interacts selectively with ASC, and this complex may function as an upstream activator of NF-kappa-B signaling.
- It inhibits TNF-alpha-induced activation & nuclear translocation of RELA/NF-KB p65, and also inhibits transcriptional activity of RELA.
- NLRP3 activates caspase-1 in response to various triggers including bacterial or viral infection, which leads to the processing and release of IL1B and IL18.
Expression
- NLRP3 is expressed in blood leukocytes and is also strongly expressed in polymorphonuclear cells and osteoblasts
- NLRP3 is undetectable or expressed at a lower magnitude in B- and T-lymphoblasts respectively
- High levels of NLRP3 expression are detected in chondrocytes
- NLRP3 is also detected in non-keratinizing epithelia of the oropharynx, esophagus, and ectocervix, and in the urothelial layer of the bladder
Isoforms
- Human: Isoform 1: 84 kD (predicted)
- Human: Isoform 2: 106 kD (predicted)
- Human: Isoform 3-6: 110-120 kD (predicted)
- Mouse: Isoform 1: 96 kD (predicted)
- Mouse: Isoform 2-4: 110-120 kD (predicted)
- Rat: Isoform 1: 119 kD (predicted)
Western blot tips
NLRP3 is regulated by post-translational modifications, such as ubiquitination and phosphorylation, and has several isoforms. Therefore, several bands may exist in a western blot assay. The predicted molecular weight of NLRP3 is about 118 kDa.
| Sample preparation |
|
|
|
| Maximizing signal |
|
| Recommended controls |
|
Find full information on working with NLRP3:
In English (link to pdf)
In Mandarin (Link to pdf)