Anti-Hemagglutinin antibody [16B12] (ab24779)

Submit a review Q&A (3)References (4)

Overview

  • Product name
    Anti-Hemagglutinin antibody [16B12]
    See all Hemagglutinin primary antibodies
  • Description
    Mouse monoclonal [16B12] to Hemagglutinin
  • Host species
    Mouse
  • Tested applications
    Suitable for: WB, ICC/IF, IP, Flow Cytmore details
  • Species reactivity
    Reacts with Influenza A virus Not yet tested in other species.
  • Immunogen

    Synthetic peptide:

    CYPYDVPDYASL

    , corresponding to amino acids 97/108 of Influenza A virus Hemagglutinin A.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Epitope
    ab24779 recognizes the influenza hemagglutinin epitope (YPYDVPDYA).

Properties

Applications

Our Abpromise guarantee covers the use of ab24779 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 63 kDa.
ICC/IF 1/1000.
IP 1/150.
Flow Cyt Use at an assay dependent concentration.

Target

  • Relevance
    Hemagglutinin (HA) is a class I viral fusion protein from Influenza virus. It is a major glycoprotein, comprising over 80% of the envelope proteins present in the virus particle. HA binds to sialic acid-containing receptors on the cell surface, bringing about the attachment of the virus particle to the cell, and is responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane. The extent of infection into host organism is determined by HA. In natural infection, inactive HA is matured into HA1 and HA2 outside the cell by one or more trypsin-like, arginine-specific endoproteases secreted by the bronchial epithelial cells. The HA protein is a homotrimer of disulfide-linked HA1-HA2. It also plays a major role in the determination of host range restriction and virulence. Genetic variation of hemagglutinin and/or neuraminidase genes results in the emergence of new influenza strains.
  • Cellular localization
    Cell membrane; apical cell membrane; single-pass type I membrane protein.
  • Alternative names
    • HA antibody
    • HA1 antibody
    • HA2 antibody
    • Hemagglutinin antibody

Images

  • Western blot - Anti-Hemagglutinin antibody [16B12] (ab24779)
    Western blot - Anti-Hemagglutinin antibody [16B12] (ab24779)
    All lanes : Anti-Hemagglutinin antibody [16B12] (ab24779)

    Lane 1 : CHO cell lysate
    Lane 2 : CHO HA cell lysate

    Secondary
    All lanes : HRP Goat anti-mouse

    Predicted band size: 63 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Hemagglutinin antibody [16B12] (ab24779)
    Immunocytochemistry/ Immunofluorescence - Anti-Hemagglutinin antibody [16B12] (ab24779)
    Immunofluorescence detection of hemagglutinin using ab24779.
  • Immunocytochemistry/ Immunofluorescence - Anti-Hemagglutinin antibody [16B12] (ab24779)
    Immunocytochemistry/ Immunofluorescence - Anti-Hemagglutinin antibody [16B12] (ab24779)

    Immunofluorescence staining of HeLa cells transfected without (A) or with (B, C) multiple tag plasmid using ab24779 (1:1000 dilution). An Alexa Fluor® 594 Goat anti-mouse IgG was used as the secondary antibody (1:250). Nuclei stained with DAPI (blue). The image was captured with a 60X objective using KEYENCE BZ-X700 fluorescence microscope.

References

This product has been referenced in:
  • Wang L  et al. Retrograde regulation of STIM1-Orai1 interaction and store-operated Ca2+ entry by calsequestrin. Sci Rep 5:11349 (2015). Read more (PubMed: 26087026) »
  • Zhuang X  et al. A BAR-domain protein SH3P2, which binds to phosphatidylinositol 3-phosphate and ATG8, regulates autophagosome formation in Arabidopsis. Plant Cell 25:4596-615 (2013). Read more (PubMed: 24249832) »
See all 4 Publications for this product

Publishing research using ab24779? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1-3 of 3 Abreviews or Q&A

Question

I have recently purchased a monoclonal AB (ab24779)that I want to label with a fluorescent marker. In order to do this, the antibody solution must be free of amines and ammonium ions. Can you confirm that ab24779 is free of these products ?

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Answer

Thank you for your enquiry. This antibody ab24779 is an ascites fluid preparation. Therefore, I cannot guarantee this will be free of amines and ammonium ions. It is not recommended for use in conjugation as it is better to use purified antibodies in conjugation experiments. I hope this helps. I'm sorry it is not better news for you. Please get back to me if you have any more questions or require any more advice. With thanks

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Question

Hi, I would like to purchase an anti-HA-antibody, and I found the following on the internet. How much do they cost, and how long is the shipping time? ab16918 ab19985 ab24779 Do they react with the following peptide? YPYDVPDYA Best regards,

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Answer

Thank you for your enquiry. Ab16918 and ab24779 will recognize HA-tag sequence you referenced. Ab19985 was raised to purified HA, and will not necessarily bind to that particular sequence. All shipping and pricing information is available on the online datasheets. I have included a link to each below. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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Question

Dear Sir/Madam, Can you tell me do you sell antibodies for the detection of influenza viruses. I am looking for HN antibodies. For example bird flu = H5N1 H can range from 1 to 16 and N from 1to 9. Therefore do you sell different HN antibodies to differentiate between different strains. I hope this makes sense Thanks

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Answer

Thank you for your enquiry. Avian Influenza A Hemagglutinin antibody (ab21292) can be used for the detection of the Hemagglutinin protein from the H5N1 strain of avian influenza A. Avian Influenza Matrix Protein antibody (ab25918) or Avian Influenza Nucleoprotien antibody (ab22285) can be applied for the detection of the H6N1 strain. I have not been able to find reference to other HN strains. Unfortunately our antibodies are not searchable by the strain that they detect. I recommend searching in google using the keywords "(strain)" together with "Abcam". I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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