Anti-Hemagglutinin antibody [16B12] (ab24779)
- Datasheet
- References (5)
- Protocols
Overview
-
Product nameAnti-Hemagglutinin antibody [16B12]
See all Hemagglutinin primary antibodies -
DescriptionMouse monoclonal [16B12] to Hemagglutinin
-
Host speciesMouse
-
Tested applicationsSuitable for: WB, ICC/IF, IP, Flow Cytmore details
-
Species reactivityReacts with Influenza A virus Not yet tested in other species.
-
Immunogen
Synthetic peptide:
CYPYDVPDYASL
, corresponding to amino acids 97/108 of Influenza A virus Hemagglutinin A. -
Epitopeab24779 recognizes the influenza hemagglutinin epitope (YPYDVPDYA).
Properties
-
FormLiquid
-
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
-
Storage bufferConstituent: Ascites
-
Concentration information loading...
-
PurityAscites
-
ClonalityMonoclonal
-
Clone number16B12
-
IsotypeIgG1
-
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
Applications
Our Abpromise guarantee covers the use of ab24779 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | 1/1000. Predicted molecular weight: 63 kDa. | |
ICC/IF | 1/1000. | |
IP | 1/150. | |
Flow Cyt | Use at an assay dependent concentration. |
Target
-
RelevanceHemagglutinin (HA) is a class I viral fusion protein from Influenza virus. It is a major glycoprotein, comprising over 80% of the envelope proteins present in the virus particle. HA binds to sialic acid-containing receptors on the cell surface, bringing about the attachment of the virus particle to the cell, and is responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane. The extent of infection into host organism is determined by HA. In natural infection, inactive HA is matured into HA1 and HA2 outside the cell by one or more trypsin-like, arginine-specific endoproteases secreted by the bronchial epithelial cells. The HA protein is a homotrimer of disulfide-linked HA1-HA2. It also plays a major role in the determination of host range restriction and virulence. Genetic variation of hemagglutinin and/or neuraminidase genes results in the emergence of new influenza strains.
-
Cellular localizationCell membrane; apical cell membrane; single-pass type I membrane protein.
-
Alternative names
- HA antibody
- HA1 antibody
- HA2 antibody
- Hemagglutinin antibody
Images
-
All lanes : Anti-Hemagglutinin antibody [16B12] (ab24779)
Lane 1 : CHO cell lysate
Lane 2 : CHO HA cell lysate
Secondary
All lanes : HRP Goat anti-mouse
Predicted band size: 63 kDa -
Immunofluorescence detection of hemagglutinin using ab24779.
-
Immunofluorescence staining of HeLa cells transfected without (A) or with (B, C) multiple tag plasmid using ab24779 (1:1000 dilution). An Alexa Fluor® 594 Goat anti-mouse IgG was used as the secondary antibody (1:250). Nuclei stained with DAPI (blue). The image was captured with a 60X objective using KEYENCE BZ-X700 fluorescence microscope.
Protocols
References
This product has been referenced in:
- Wang L et al. Retrograde regulation of STIM1-Orai1 interaction and store-operated Ca2+ entry by calsequestrin. Sci Rep 5:11349 (2015). Read more (PubMed: 26087026) »
- Zhuang X et al. A BAR-domain protein SH3P2, which binds to phosphatidylinositol 3-phosphate and ATG8, regulates autophagosome formation in Arabidopsis. Plant Cell 25:4596-615 (2013). Read more (PubMed: 24249832) »