Hematoxylin (Modified Mayer's) (ab220365)


  • Product name
    Hematoxylin (Modified Mayer's)
  • Tested applications
    Suitable for: IHCmore details
  • General notes

    Hematoxylin is a nuclear stain that is used as a counterstain for immunohistochemistry (IHC). Together with eosin, it forms the Hematoxylin and Eosin stain (H&E stain), one of the most commonly used stains in histology. This product is a high quality, ready-to-use solution of a commonly used formulation called Modified Mayer's hematoxylin (Lille's modification). 

    Other products for IHC

    Find kits and reagents for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the IHC kits and reagents guide.


  • Storage instructions
    Store at room temperature.
  • Concentration information loading...


Our Abpromise guarantee covers the use of ab220365 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC Use at an assay dependent concentration.


  • Immunohistochemistry Staining with CD34 on Human tonsil. Counterstained with Hematoxylin (Modified Mayer's) (ab220365) for 30 seconds followed by bluing with Bluing Reagent. 

  • H&E Stain Kit (Hematoxylin and Eosin) staining formalin-fixed-paraffin embedded tissue section.

    ab220365 is used as a component of H&E Stain Kit (Hematoxylin and Eosin) (ab245880)

  • Pig Lung stained with Hematoxylin (Modified Mayer's) (ab220365) followed by bluing with Bluing Reagent. 


This product has been referenced in:
  • Du B  et al. CD4+ aß T cell infiltration into the leptomeninges of lumbar dorsal roots contributes to the transition from acute to chronic mechanical allodynia after adult rat tibial nerve injuries. J Neuroinflammation 15:81 (2018). Read more (PubMed: 29544518) »
  • Li Y  et al. A novel model of persistent retinal neovascularization for the development of sustained anti-VEGF therapies. Exp Eye Res 174:98-106 (2018). IHC-P . Read more (PubMed: 29852133) »
See all 3 Publications for this product

Customer reviews and Q&As


It’s not so much how to dilute the hematoxylin, but how strong you’d like the staining.

I like a really light stain (especially when doing IHC with HRP / DAB), so 5 seconds (or so) is all I expose the section to before transferring to running water. The degree of staining over any given time period will vary according to several parameters (for example, batch-to-batch variation, temperature, specimen type, etc).

If I were the customer, I take some of the specimens that they were planning to test, and stain for 5, 10, 15, 30 and 60 seconds, to see what degree of staining pleases them, then use this for their actual tests.

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