Product nameHeme Assay Kit
Sample typeUrine, Serum, Plasma, Whole Blood
Range0.6 µM - 125 µM
Heme Assay Kit (ab272534) is a simple, direct and automation-ready procedure for measuring heme concentration. This Assay Kit is based on an improved aqueous alkaline solution method, in which the heme is converted into a uniform colored form. The intensity of color, measured at 400 nm, is directly proportional to the heme concentration in the sample. The optimized formulation substantially reduces interference by substances in the raw samples and exhibits high sensitivity.
Sensitive and accurate: Linear detection range 0.6 – 125 μM heme in 96-well plate assay.
Simple and high-throughput: The “mix-and-read” procedure involves addition of a single working reagent and reading the optical density. Can be readily automated as a high-throughput assay in 96-well plates for thousands of samples per day.
Safety: Reagents are non-toxic.
Versatility: Assays can be executed in 96-well plate or cuvette.
Tested applicationsSuitable for: Functional Studiesmore details
Storage instructionsStore at +4°C. Please refer to protocols.
Components 250 tests Calibrator 1 x 10ml Reagent 1 x 50ml
Our Abpromise guarantee covers the use of ab272534 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
ab272534 has not yet been referenced specifically in any publications.