Anti-Heme Oxygenase 1 antibody (ab137749)
Key features and details
- Rabbit polyclonal to Heme Oxygenase 1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Heme Oxygenase 1 antibody
See all Heme Oxygenase 1 primary antibodies -
Description
Rabbit polyclonal to Heme Oxygenase 1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment, corresponding to a region within amino acids 1-278 of Human Heme Oxygenase 1.
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Positive control
- H1299, HeLa, NIH-3T3, JC, BCL-1, C2C12 and Raw264.7 whole cell lysates, OV90 xenograft tissue.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab137749 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 33 kDa. | |
| IHC-P | 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Alternatively antigen retrieval using heat mediated Tris/EDTA buffer (pH 8.0) may be used. | |
| ICC/IF | 1/100 - 1/1000. |
Target
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Function
Heme oxygenase cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed. -
Sequence similarities
Belongs to the heme oxygenase family. -
Cellular localization
Microsome. Endoplasmic reticulum. - Information by UniProt
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Database links
- Entrez Gene: 3162 Human
- Entrez Gene: 15368 Mouse
- Omim: 141250 Human
- SwissProt: P09601 Human
- SwissProt: P14901 Mouse
- Unigene: 517581 Human
- Unigene: 276389 Mouse
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Alternative names
- 32 kD antibody
- bK286B10 antibody
- D8Wsu38e antibody
see all
Images
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Western blot - Anti-Heme Oxygenase 1 antibody (ab137749)All lanes : Anti-Heme Oxygenase 1 antibody (ab137749) at 1/500 dilution
Lane 1 : Wile-type HeLa cell lysate
Lane 2 : Knockout Hela cell lysates
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 33 kDa
Observed band size: 33 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Heme Oxygenase 1 antibody (ab137749)Immunohistochemical analysis of human renal carcinaoma using ab137749 at 1/500.
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Immunocytochemistry/ Immunofluorescence - Anti-Heme Oxygenase 1 antibody (ab137749)Immunofluorescent analysis of A375 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ab137749 diluted at 1:500.
Blue: Hoechst 33342 staining. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Heme Oxygenase 1 antibody (ab137749)Immunohistochemical analysis of rat spleen using ab137749 at 1/500.
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Western blot - Anti-Heme Oxygenase 1 antibody (ab137749)All lanes : Anti-Heme Oxygenase 1 antibody (ab137749) at 1/5000 dilution
Lane 1 : Untreated HeLa whole cell extracts
Lane 2 : Treated HeLa whole cell extracts
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 33 kDa -
Western blot - Anti-Heme Oxygenase 1 antibody (ab137749)All lanes : Anti-Heme Oxygenase 1 antibody (ab137749) at 1/1000 dilution
Lane 1 : H1299 cell lysate
Lane 2 : HeLa cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 33 kDa
12% SDS PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Heme Oxygenase 1 antibody (ab137749)Immunohistochemical analysis of paraffin-embedded OV90 xenograft, labelling Heme Oxygenase 1 using ab137749 at 1/100 dilution. -
Western blot - Anti-Heme Oxygenase 1 antibody (ab137749)All lanes : Anti-Heme Oxygenase 1 antibody (ab137749) at 1/1000 dilution
Lane 1 : NIH-3T3 whole cell lysate
Lane 2 : JC whole cell lysate
Lane 3 : BCL1 whole cell lysate
Lane 4 : C2C12 whole cell lysate
Lane 5 : Raw624.7 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 33 kDa
12% SDS PAGE
Protocols
References (13)
ab137749 has been referenced in 13 publications.
- Wang Z et al. Aronia melanocarpa Prevents Alcohol-Induced Chronic Liver Injury via Regulation of Nrf2 Signaling in C57BL/6 Mice. Oxid Med Cell Longev 2020:4054520 (2020). PubMed: 31998436
- Liang Q et al. Stigmasterol alleviates cerebral ischemia/reperfusion injury by attenuating inflammation and improving antioxidant defenses in rats. Biosci Rep 40:N/A (2020). PubMed: 32149332
- Li D et al. Astragalus polysaccharide alleviates H2O2-triggered oxidative injury in human umbilical vein endothelial cells via promoting KLF2. Artif Cells Nanomed Biotechnol 47:2188-2195 (2019). PubMed: 31159593
- Polo S et al. Treatment With the Delta Opioid Agonist UFP-512 Alleviates Chronic Inflammatory and Neuropathic Pain: Mechanisms Implicated. Front Pharmacol 10:283 (2019). PubMed: 30971925
- Meng B et al. Hepatoprotective Effects of Morchella esculenta against Alcohol-Induced Acute Liver Injury in the C57BL/6 Mouse Related to Nrf-2 and NF-?B Signaling. Oxid Med Cell Longev 2019:6029876 (2019). PubMed: 31396303
