Key features and details
- Rabbit polyclonal to Heme Oxygenase 1
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Heme Oxygenase 1 antibody
See all Heme Oxygenase 1 primary antibodies
DescriptionRabbit polyclonal to Heme Oxygenase 1
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla
Synthetic peptide within Human Heme Oxygenase 1 aa 238-288. The exact sequence is proprietary. (NP_002124.1).
Database link: P09601
- WB: HeLa and HEK-293T whole cell lysates. IP: HeLa whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab225996 was affinity purified using an epitope specific to Heme Oxygenase 1 immobilized on solid support.
Our Abpromise guarantee covers the use of ab225996 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 33 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionHeme oxygenase cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed.
Sequence similaritiesBelongs to the heme oxygenase family.
Cellular localizationMicrosome. Endoplasmic reticulum.
- Information by UniProt
- 32 kD antibody
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All lanes : Anti-Heme Oxygenase 1 antibody (ab225996) at 0.1 mg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Lane 4 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 33 kDa
Exposure time: 3 minutes
Heme Oxygenase 1 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab225996 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab225996 at 1 µg/ml.
Lane 1: ab225996 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 30 seconds.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab225996 has not yet been referenced specifically in any publications.