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    heme-oxygenase-1-antibody-ep1391y-ab52947.pdf

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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-Heme Oxygenase 1 antibody [EP1391Y] (ab52947)

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  • SDS
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Western blot - Anti-Heme Oxygenase 1 antibody [EP1391Y] (ab52947)
  • Flow Cytometry (Intracellular) - Anti-Heme Oxygenase 1 antibody [EP1391Y] (ab52947)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Heme Oxygenase 1 antibody [EP1391Y] (ab52947)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Heme Oxygenase 1 antibody [EP1391Y] (ab52947)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Heme Oxygenase 1 antibody [EP1391Y] (ab52947)
  • Western blot - Anti-Heme Oxygenase 1 antibody [EP1391Y] (ab52947)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Heme Oxygenase 1 antibody [EP1391Y] (ab52947)
  • Immunoprecipitation - Anti-Heme Oxygenase 1 antibody [EP1391Y] (ab52947)
  • Flow Cytometry (Intracellular) - Anti-Heme Oxygenase 1 antibody [EP1391Y] (ab52947)
  • Western blot - Anti-Heme Oxygenase 1 antibody [EP1391Y] (ab52947)
  • Anti-Heme Oxygenase 1 antibody [EP1391Y] (ab52947)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1391Y] to Heme Oxygenase 1
  • Suitable for: Flow Cyt (Intra), WB, IHC-P, IP
  • Knockout validated
  • Reacts with: Mouse, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 APC Carrier Free HRP PE

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Anti-Heme Oxygenase 1 antibody [EP1391Y] - BSA and Azide free (ab219360)

View more associated products

Overview

  • Product name

    Anti-Heme Oxygenase 1 antibody [EP1391Y]
    See all Heme Oxygenase 1 primary antibodies
  • Description

    Rabbit monoclonal [EP1391Y] to Heme Oxygenase 1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt (Intra), WB, IHC-P, IPmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Guinea pig
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Fetal liver lysate, human liver tissue, A549, HL-60, MCF7, NIH/3T3, HEK-293, and HeLa cells. IHC-P: FFPE mouse spleen normal. Human liver and human and mouse spleen tissue. Human spleen tissue. IP: Mouse spleen tissue lysate Flow Cyt (intra): NIH/3T3 cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EP1391Y
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Alzheimer's disease
    • Other
    • Cardiovascular
    • Blood
    • Platelets
    • Signal Transduction
    • Signaling Pathway
    • Nuclear Signaling
    • NFkB Pathway
    • Epigenetics and Nuclear Signaling
    • Nuclear Signaling Pathways
    • NFkB pathway
    • Cardiovascular
    • Atherosclerosis
    • Vascular Inflammation
    • Inflammatory mediators
    • Cardiovascular
    • Vasculature
    • Endothelium
    • Cancer
    • Cancer Metabolism
    • Response to hypoxia
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Hypoxia
    • Metabolism
    • Types of disease
    • Neurodegenerative disease
    • Metabolism
    • Types of disease
    • Cancer
    • Metabolism
    • Types of disease
    • Heart disease

Associated products

  • Alternative Versions

    • Anti-Heme Oxygenase 1 antibody [EP1391Y] - BSA and Azide free (ab219360)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed (ab96899)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • KO cell lines

    • Human HMOX1 (Heme Oxygenase 1) knockout A549 cell line (ab269503)
  • KO cell lysates

    • Human HMOX1 (Heme Oxygenase 1) knockout A549 cell lysate (ab269665)
  • Recombinant Protein

    • Recombinant Human Heme Oxygenase 1 protein (ab85243)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab52947 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/1000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

WB (4)
1/2000. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa).
IHC-P
Use a concentration of 0.1 - 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP
1/30.
Notes
Flow Cyt (Intra)
1/1000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

WB
1/2000. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa).
IHC-P
Use a concentration of 0.1 - 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP
1/30.

Target

  • Function

    Heme oxygenase cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed.
  • Sequence similarities

    Belongs to the heme oxygenase family.
  • Cellular localization

    Microsome. Endoplasmic reticulum.
  • Target information above from: UniProt accession P09601 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 3162 Human
    • Entrez Gene: 15368 Mouse
    • Omim: 141250 Human
    • SwissProt: P09601 Human
    • SwissProt: P14901 Mouse
    • Unigene: 517581 Human
    • Unigene: 276389 Mouse
    • Alternative names

      • 32 kD antibody
      • bK286B10 antibody
      • D8Wsu38e antibody
      • heat shock protein 32 kD antibody
      • heat shock protein 32kD antibody
      • Heat shock protein antibody
      • Heme oxygenase (decycling) 1 antibody
      • Heme oxygenase 1 antibody
      • Hemox antibody
      • HMOX 1 antibody
      • Hmox antibody
      • Hmox1 antibody
      • HMOX1_HUMAN antibody
      • HO 1 antibody
      • HO antibody
      • HO-1 antibody
      • HO1 antibody
      • Hsp32 antibody
      see all

    Images

    • Western blot - Anti-Heme Oxygenase 1 antibody [EP1391Y] (ab52947)
      Western blot - Anti-Heme Oxygenase 1 antibody [EP1391Y] (ab52947)
      All lanes : Anti-Heme Oxygenase 1 antibody [EP1391Y] (ab52947) at 1/2000 dilution

      Lane 1 : Wild-type A549 cell lysate
      Lane 2 : HMOX1 knockout A549 cell lysate
      Lane 3 : Human Spleen tissue lysate
      Lane 4 : HL-60 cell lysate
      Lane 5 : MCF7 cell lysate
      Lane 6 : HeLa cell lysate
      Lane 7 : A549 cell lysate

      Lysates/proteins at 20 µg per lane.

      Performed under reducing conditions.

      Predicted band size: 33 kDa
      Observed band size: 33 kDa



      Lanes 1 - 7: Merged signal (red and green). Green - ab52947 observed at 33 kDa. Red - loading control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) observed at 55 kDa.

      ab52947 was shown to react with Heme Oxygenase 1 in wild-type A549 cells in Western blot with loss of signal observed in HMOX1 knockout cell line ab269503 (knockout cell lysate ab269665). Wild-type A549 and HMOX1 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with ab52947 and ab7291 (Mouse anti-Alpha Tubulin [DM1A]) overnight at 4 °C at a 1 in 2000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.

    • Flow Cytometry (Intracellular) - Anti-Heme Oxygenase 1 antibody [EP1391Y] (ab52947)
      Flow Cytometry (Intracellular) - Anti-Heme Oxygenase 1 antibody [EP1391Y] (ab52947)

      Intracellular flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized NIH/3T3 (Mouse embryonic fibroblast) cells labelling Heme Oxygenase 1 with ab52947 at 1/50 dilution (1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor 488, ab150077) at 1/2000 dilution was used as the secondary antibody. 

       

       

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Heme Oxygenase 1 antibody [EP1391Y] (ab52947)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Heme Oxygenase 1 antibody [EP1391Y] (ab52947)

      Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling Heme Oxygenase 1 with ab52947 at 1/2000 (0.246 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used. Cytoplasmic staining on human spleen. The section was incubated with ab52947 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND RX instrument Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used.

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Heme Oxygenase 1 antibody [EP1391Y] (ab52947)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Heme Oxygenase 1 antibody [EP1391Y] (ab52947)

      Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling Heme Oxygenase 1 with ab52947 at 1/2000 (0.246 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used. Cytoplasmic staining on mouse spleen. The section was incubated with ab52947 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND RX instrument Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used.

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Heme Oxygenase 1 antibody [EP1391Y] (ab52947)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Heme Oxygenase 1 antibody [EP1391Y] (ab52947)

      Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Heme Oxygenase 1 with ab52947 at 1/2000 (0.246 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used. Cytoplasmic staining on Kupffer cells in human liver. The section was incubated with ab52947 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND RX instrument Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used.

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

    • Western blot - Anti-Heme Oxygenase 1 antibody [EP1391Y] (ab52947)
      Western blot - Anti-Heme Oxygenase 1 antibody [EP1391Y] (ab52947)
      All lanes : Anti-Heme Oxygenase 1 antibody [EP1391Y] (ab52947) at 1/1000 dilution

      Lane 1 : Hek293
      Lane 2 : HL60
      Lane 3 : HeLa
      Lane 4 : A549
      Lane 5 : Hu spleen
      Lane 6 : Ms spleen
      Lane 7 : Rt spleen

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : IRDye® 800CW Goat anti Rabbit

      Predicted band size: 33 kDa
      Observed band size: 32 kDa why is the actual band size different from the predicted?



      Hek293 & HL60 presumed negative or very low expression.

      Loading control GAPDH at 38kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Heme Oxygenase 1 antibody [EP1391Y] (ab52947)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Heme Oxygenase 1 antibody [EP1391Y] (ab52947)

      IHC image of ab52947 staining in mouse spleen formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab52947, 5µg/ml, for 15 mins at room temperature. A goat anti-rabbit biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    • Immunoprecipitation - Anti-Heme Oxygenase 1 antibody [EP1391Y] (ab52947)
      Immunoprecipitation - Anti-Heme Oxygenase 1 antibody [EP1391Y] (ab52947)

      Heme Oxygenase 1 was immunoprecipitated from 0.35mg mouse spleen lysate with ab52947 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab52947 at 1/1000 dilution (1 μg/mL). VeriBlot for IP Detection Reagent (HRP) (ab131366) was used as the secondary antibody at 1/1000 dilution.

      Lane 1: Mouse spleen tissue lysate 10 μg
      Lane 2: Mouse spleen tissue lysate
      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab52947 in mouse spleen lysate.

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    • Flow Cytometry (Intracellular) - Anti-Heme Oxygenase 1 antibody [EP1391Y] (ab52947)
      Flow Cytometry (Intracellular) - Anti-Heme Oxygenase 1 antibody [EP1391Y] (ab52947)

      Overlay histogram showing HEK-293 (human epithelial cell line from embryonic kidney) cells stained with ab52947 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab52947, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HEK293 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions. 

       

       

    • Western blot - Anti-Heme Oxygenase 1 antibody [EP1391Y] (ab52947)
      Western blot - Anti-Heme Oxygenase 1 antibody [EP1391Y] (ab52947)
      All lanes : Anti-Heme Oxygenase 1 antibody [EP1391Y] (ab52947) at 1/1000 dilution

      Lane 1 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysates with 5% NFDM/TBST
      Lane 2 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysates with 5% NFDM/TBST
      Lane 3 : A549 (Human lung carcinoma epithelial cell) whole cell lysates with 5% NFDM/TBST
      Lane 4 : Mouse spleen lysates with 5% NFDM/TBST

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 33 kDa
      Observed band size: 33 kDa


      Exposure time: 120 seconds


      We are unsure how to define the extra bands

    • Anti-Heme Oxygenase 1 antibody [EP1391Y] (ab52947)
      Anti-Heme Oxygenase 1 antibody [EP1391Y] (ab52947)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (52)

    Publishing research using ab52947? Please let us know so that we can cite the reference in this datasheet.

    ab52947 has been referenced in 52 publications.

    • Lidberg KA  et al. Antisense oligonucleotide development for the selective modulation of CYP3A5 in renal disease. Sci Rep 11:4722 (2021). PubMed: 33633318
    • Goonetilleke M  et al. Addressing the liver progenitor cell response and hepatic oxidative stress in experimental non-alcoholic fatty liver disease/non-alcoholic steatohepatitis using amniotic epithelial cells. Stem Cell Res Ther 12:429 (2021). PubMed: 34321089
    • Luo W  et al. HO-1 nuclear accumulation and interaction with NPM1 protect against stress-induced endothelial senescence independent of its enzymatic activity. Cell Death Dis 12:738 (2021). PubMed: 34312365
    • Stucki D  et al. Endogenous Carbon Monoxide Signaling Modulates Mitochondrial Function and Intracellular Glucose Utilization: Impact of the Heme Oxygenase Substrate Hemin. Antioxidants (Basel) 9:N/A (2020). PubMed: 32717801
    • Fang W  et al. Molecular Hydrogen Protects Human Melanocytes from Oxidative Stress by Activating Nrf2 Signaling. J Invest Dermatol 140:2230-2241.e9 (2020). PubMed: 32234461
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
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    1-5 of 5 Abreviews or Q&A

    Western blot abreview for Anti-Heme Oxygenase 1 [EP1391Y] antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    10 µg
    Gel Running Conditions
    Reduced Denaturing
    Sample
    Mouse Cell lysate - whole cell (RAW 264.7)
    Specification
    RAW 264.7
    Treatment
    Control No treatment, 5mM N-ACETYL CYSTEINE, 50uM buthionine sulphoximine
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Vamsi Kodali

    Verified customer

    Submitted Jun 06 2014

    Western blot abreview for Anti-Heme Oxygenase 1 antibody [EP1391Y]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (C42B Prostate cancer cell line)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    20 µg
    Specification
    C42B Prostate cancer cell line
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Dimitra Kalamida

    Verified customer

    Submitted Aug 07 2019

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Heme Oxygenase 1 antibody [EP1391Y]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (C42B Prostate cancer cell line)
    Permeabilization
    Yes - 0.1% Triton X-100
    Specification
    C42B Prostate cancer cell line
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Dimitra Kalamida

    Verified customer

    Submitted Aug 02 2019

    Western blot abreview for Anti-Heme Oxygenase 1 antibody [EP1391Y]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    40 µg
    Gel Running Conditions
    Reduced Denaturing (8%)
    Sample
    Guinea pig Tissue lysate - whole (liver and brain)
    Specification
    liver and brain
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Jul 02 2013

    Western blot abreview for Anti-Heme Oxygenase 1 antibody [EP1391Y]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (endothelial cells)
    Loading amount
    10000 cells
    Specification
    endothelial cells
    Gel Running Conditions
    Reduced Denaturing (12% gel)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Mar 12 2013

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