Recombinant
RabMAb

Anti-Heme Oxygenase 1 antibody [EPR1390Y] (ab68477)

Abreviews (3) Submit a Question Specific References (18)

Overview

  • Product name
    Anti-Heme Oxygenase 1 antibody [EPR1390Y]
    See all Heme Oxygenase 1 primary antibodies
  • Description
    Rabbit monoclonal [EPR1390Y] to Heme Oxygenase 1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, WB, IP, Flow Cytmore details
    Unsuitable for: IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    within Human Heme Oxygenase 1 aa 1-100 (N terminal). The exact sequence is proprietary.
    Database link: P09601

  • Positive control
    • HepG2, A549, rat kidney, rat spleen, mouse kidney cell lysate.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab68477 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/250.
WB 1/10000 - 1/50000. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa).
IP 1/20.
Flow Cyt Use at an assay dependent concentration.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
  • Application notes
    Is unsuitable for IHC-P.

    • Target

    • Function
      Heme oxygenase cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed.
    • Sequence similarities
      Belongs to the heme oxygenase family.
    • Cellular localization
      Microsome. Endoplasmic reticulum.
    • Information by UniProt
    • Database links
      see all
    • Alternative names
      • 32 kD antibody
      • bK286B10 antibody
      • D8Wsu38e antibody
      • heat shock protein 32 kD antibody
      • heat shock protein 32kD antibody
      • Heat shock protein antibody
      • Heme oxygenase (decycling) 1 antibody
      • Heme oxygenase 1 antibody
      • Hemox antibody
      • HMOX 1 antibody
      • Hmox antibody
      • Hmox1 antibody
      • HMOX1_HUMAN antibody
      • HO 1 antibody
      • HO antibody
      • HO-1 antibody
      • HO1 antibody
      • Hsp32 antibody
      see all

    Images

    • Western blot - Anti-Heme Oxygenase 1 antibody [EPR1390Y] (ab68477)
      Western blot - Anti-Heme Oxygenase 1 antibody [EPR1390Y] (ab68477)
      All lanes : Anti-Heme Oxygenase 1 antibody [EPR1390Y] (ab68477) at 1/1000 dilution

      Lane 1 : Hek293
      Lane 2 : HL60
      Lane 3 : HeLa
      Lane 4 : A549
      Lane 5 : Hu spleen
      Lane 6 : Ms spleen
      Lane 7 : Rt spleen

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : IRDye® 800CW Goat anti Rabbit

      Predicted band size: 33 kDa



      Hek293 & HL60 presumed negative or very low expression.

      Loading control GAPDH at 38kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-Heme Oxygenase 1 antibody [EPR1390Y] (ab68477)
      Immunocytochemistry/ Immunofluorescence - Anti-Heme Oxygenase 1 antibody [EPR1390Y] (ab68477)This image is courtesy of an anonymous Abreview

      ab68477 (unpurified) staining Heme Oxygenase 1 in mouse embryonic fibroblasts by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilized with 0.2% Triton X-100 and blocked with 1.5% serum for 10 minutes at 25°C. Samples were incubated with primary antibody (1/100 in 1X HBSS + 0.02% Triton X-100 + 1.5% FBS) for 3 hours at 25°C. An Alexa Fluor® 594-conjugated goat anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody.

      See Abreview

    • Immunocytochemistry/ Immunofluorescence - Anti-Heme Oxygenase 1 antibody [EPR1390Y] (ab68477)
      Immunocytochemistry/ Immunofluorescence - Anti-Heme Oxygenase 1 antibody [EPR1390Y] (ab68477)

      Immunocytochemistry/Immunofluorescence analysis of HepG2 (Human liver hepatocellular carcinoma cell line) cells labeling Heme Oxygenase 1 with purified ab68477 at 1/100. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000. DAPI was used as a nuclear counterstain.

      Negative control 1: PBS only.

    • Flow Cytometry - Anti-Heme Oxygenase 1 antibody [EPR1390Y] (ab68477)
      Flow Cytometry - Anti-Heme Oxygenase 1 antibody [EPR1390Y] (ab68477)

      Flow cytometry analysis of A549 (human lung carcinoma) cells labeling with purified ab68477 at 1/200 dilution ( 1ug/ml) (Red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(ab150077) )(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black)(ab172730) was used as a isotype control.Cell without incubation with primary antibody and secondary antibody (Blue) were used as unlabeled control.

    • Immunoprecipitation - Anti-Heme Oxygenase 1 antibody [EPR1390Y] (ab68477)
      Immunoprecipitation - Anti-Heme Oxygenase 1 antibody [EPR1390Y] (ab68477)

      ab68477 (purified) at 1/20 immunoprecipitating Heme Oxygenase 1 in A549 (Human lung carcinoma cell line) whole cell lysate.

      Lane 1 (input): A549 whole cell lysate (10ug).

      Lane 2 (+): ab68477 + A549 whole cell lysate.

      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab133267 in HeLa whole cell lysate.

      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST.

    • Western blot - Anti-Heme Oxygenase 1 antibody [EPR1390Y] (ab68477)
      Western blot - Anti-Heme Oxygenase 1 antibody [EPR1390Y] (ab68477)
      All lanes : Anti-Heme Oxygenase 1 antibody [EPR1390Y] (ab68477) at 1/20000 dilution (purified)

      Lane 1 : Rat kidney lysate
      Lane 2 : Rat spleen lysate
      Lane 3 : Mouse kidney lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

      Predicted band size: 33 kDa
      Observed band size: 33 kDa



      Blocking and dilution buffer: 5% NFDM/TBST

    • Western blot - Anti-Heme Oxygenase 1 antibody [EPR1390Y] (ab68477)
      Western blot - Anti-Heme Oxygenase 1 antibody [EPR1390Y] (ab68477)
      All lanes : Anti-Heme Oxygenase 1 antibody [EPR1390Y] (ab68477) at 1/20000 dilution (purified)

      Lane 1 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
      Lane 2 : A549 (Human lung carcinoma cell line) whole cell lysate

      Lysates/proteins at 15 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

      Predicted band size: 33 kDa
      Observed band size: 33 kDa



      Blocking and dilution buffer: 5% NFDM/TBST

    • Western blot - Anti-Heme Oxygenase 1 antibody [EPR1390Y] (ab68477)
      Western blot - Anti-Heme Oxygenase 1 antibody [EPR1390Y] (ab68477)
      All lanes : Anti-Heme Oxygenase 1 antibody [EPR1390Y] (ab68477) at 1/2000 dilution (purified)

      Lane 1 : HepG2 cell lysate
      Lane 2 : A549 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP conjugated Goat anti-Rabbit at 1/2000 dilution

      Predicted band size: 33 kDa
      Observed band size: 33 kDa
      Additional bands at: 125-130 kDa, 30 kDa, 48 kDa, 52 kDa. We are unsure as to the identity of these extra bands.

    References

    This product has been referenced in:
    • Liu S  et al. Gastrodin protects MC3T3-E1 osteoblasts from dexamethasone-induced cellular dysfunction and promotes bone formation via induction of the NRF2 signaling pathway. Int J Mol Med 41:2059-2069 (2018). Read more (PubMed: 29393365) »
    • Feng L  et al. Icariside II alleviates oxygen-glucose deprivation and reoxygenation-induced PC12 cell oxidative injury by activating Nrf2/SIRT3 signaling pathway. Biomed Pharmacother 103:9-17 (2018). Read more (PubMed: 29635133) »

    See all 18 Publications for this product

    Publishing research using ab68477? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

    Submit a review Submit a question

    3 reviews or Q&A

    10
    Votes: Highest First

    Western blot abreview for Anti-Heme Oxygenase 1 antibody [EPR1390Y]

     Good
    Abreviews
    Application
    Western blot
    Sample
    Human Tissue lysate - whole (brain)
    Gel Running Conditions
    Reduced Denaturing (12)
    Loading amount
    50 µg
    Specification
    brain
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
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    Abcam user community

    Verified customer

    Submitted May 13 2016

    Western blot abreview for Anti-Heme Oxygenase 1 antibody [EPR1390Y]

     Excellent
    Abreviews
    Application
    Western blot
    Sample
    African Green Monkey Cell lysate - whole cell (COS-7 cells)
    Gel Running Conditions
    Reduced Denaturing (4-12% Bis-Tris)
    Loading amount
    100000 cells
    Treatment
    30 nM Bortezomib for 24 hours
    Specification
    COS-7 cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10%
    Read More
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    Abcam user community

    Verified customer

    Submitted Jun 22 2015

    Western blot abreview for Anti-Heme Oxygenase 1 antibody [EPR1390Y]

     Good
    Abreviews
    Application
    Western blot
    Loading amount
    40 µg
    Gel Running Conditions
    Reduced Denaturing (10 % gel)
    Sample
    Rat Tissue lysate - whole (aorta)
    Specification
    aorta
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More
    abreview image
    Username

    Abcam user community

    Verified customer

    Submitted Oct 22 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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