Recombinant
RabMAb

Recombinant Anti-Heme Oxygenase 1 antibody [EPR1390Y] (ab68477)

Reviews (3) Submit a question References (52)

Overview

  • Product name

    Anti-Heme Oxygenase 1 antibody [EPR1390Y]
    See all Heme Oxygenase 1 primary antibodies

  • Description

    Rabbit monoclonal [EPR1390Y] to Heme Oxygenase 1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, WB, IP, Flow Cytmore details
    Unsuitable for: IHC-P

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    within Human Heme Oxygenase 1 aa 1-100 (N terminal). The exact sequence is proprietary.
    Database link: P09601

  • Positive control

    • HepG2, A549, rat kidney, rat spleen, mouse kidney cell lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Applications

Our Abpromise guarantee covers the use of ab68477 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/250.
WB 1/10000 - 1/50000. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa).
IP 1/20.
Flow Cyt Use at an assay dependent concentration.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • Application notes
    Is unsuitable for IHC-P.

    • Target

    • Function

      Heme oxygenase cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed.

    • Sequence similarities

      Belongs to the heme oxygenase family.

    • Cellular localization

      Microsome. Endoplasmic reticulum.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • 32 kD antibody
      • bK286B10 antibody
      • D8Wsu38e antibody
      • heat shock protein 32 kD antibody
      • heat shock protein 32kD antibody
      • Heat shock protein antibody
      • Heme oxygenase (decycling) 1 antibody
      • Heme oxygenase 1 antibody
      • Hemox antibody
      • HMOX 1 antibody
      • Hmox antibody
      • Hmox1 antibody
      • HMOX1_HUMAN antibody
      • HO 1 antibody
      • HO antibody
      • HO-1 antibody
      • HO1 antibody
      • Hsp32 antibody
      see all

    Images

    • Western blot - Anti-Heme Oxygenase 1 antibody [EPR1390Y] (ab68477)
      Western blot - Anti-Heme Oxygenase 1 antibody [EPR1390Y] (ab68477)
      All lanes : Anti-Heme Oxygenase 1 antibody [EPR1390Y] (ab68477) at 1/1000 dilution

      Lane 1 : Hek293
      Lane 2 : HL60
      Lane 3 : HeLa
      Lane 4 : A549
      Lane 5 : Hu spleen
      Lane 6 : Ms spleen
      Lane 7 : Rt spleen

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : IRDye® 800CW Goat anti Rabbit

      Predicted band size: 33 kDa
      Observed band size: 32 kDa
      why is the actual band size different from the predicted?



      Hek293 & HL60 presumed negative or very low expression.

      Loading control GAPDH at 38kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-Heme Oxygenase 1 antibody [EPR1390Y] (ab68477)
      Immunocytochemistry/ Immunofluorescence - Anti-Heme Oxygenase 1 antibody [EPR1390Y] (ab68477)

      Immunocytochemistry/Immunofluorescence analysis of HepG2 (Human liver hepatocellular carcinoma cell line) cells labeling Heme Oxygenase 1 with purified ab68477 at 1/100. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000. DAPI was used as a nuclear counterstain.

      Negative control 1: PBS only.

    • Western blot - Anti-Heme Oxygenase 1 antibody [EPR1390Y] (ab68477)
      Western blot - Anti-Heme Oxygenase 1 antibody [EPR1390Y] (ab68477)
      All lanes : Anti-Heme Oxygenase 1 antibody [EPR1390Y] (ab68477) at 1/1000 dilution

      Lane 1 : HEK-293 (Human embryonic kidney epithelial cell) whole cell lysates with 5% NFDM/TBST
      Lane 2 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysates with 5% NFDM/TBST
      Lane 3 : A549 (Human lung carcinoma epithelial cell) whole cell lysates with 5% NFDM/TBST
      Lane 4 : Mouse spleen lysates with 5% NFDM/TBST

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 33 kDa
      Observed band size: 33 kDa


      Exposure time: 10 seconds


      We are unsure how to define the extra bands.

    • Flow Cytometry - Anti-Heme Oxygenase 1 antibody [EPR1390Y] (ab68477)
      Flow Cytometry - Anti-Heme Oxygenase 1 antibody [EPR1390Y] (ab68477)

      Flow cytometry analysis of A549 (human lung carcinoma) cells labeling with purified ab68477 at 1/200 dilution ( 1ug/ml) (Red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(ab150077) )(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black)(ab172730) was used as a isotype control.Cell without incubation with primary antibody and secondary antibody (Blue) were used as unlabeled control.

    • Immunoprecipitation - Anti-Heme Oxygenase 1 antibody [EPR1390Y] (ab68477)
      Immunoprecipitation - Anti-Heme Oxygenase 1 antibody [EPR1390Y] (ab68477)

      ab68477 (purified) at 1/20 immunoprecipitating Heme Oxygenase 1 in A549 (Human lung carcinoma cell line) whole cell lysate.

      Lane 1 (input): A549 whole cell lysate (10ug).

      Lane 2 (+): ab68477 + A549 whole cell lysate.

      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab133267 in HeLa whole cell lysate.

      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST.

    • Western blot - Anti-Heme Oxygenase 1 antibody [EPR1390Y] (ab68477)
      Western blot - Anti-Heme Oxygenase 1 antibody [EPR1390Y] (ab68477)
      All lanes : Anti-Heme Oxygenase 1 antibody [EPR1390Y] (ab68477) at 1/20000 dilution (purified)

      Lane 1 : Rat kidney lysate
      Lane 2 : Rat spleen lysate
      Lane 3 : Mouse kidney lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

      Predicted band size: 33 kDa
      Observed band size: 33 kDa



      Blocking and dilution buffer: 5% NFDM/TBST

    References

    This product has been referenced in:

    • Yamamoto H  et al. Heme oxygenase-1 prevents glucocorticoid and hypoxia-induced apoptosis and necrosis of osteocyte-like cells. Med Mol Morphol N/A:N/A (2019). Read more (PubMed: 30706191) »
    • Magierowska K  et al. Time-dependent course of gastric ulcer healing and molecular markers profile modulated by increased gastric mucosal content of carbon monoxide released from its pharmacological donor. Biochem Pharmacol 163:71-83 (2019). Read more (PubMed: 30753813) »
    See all 52 Publications for this product

    Publishing research using ab68477? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-3 of 3 Abreviews or Q&A

    Western blot abreview for Anti-Heme Oxygenase 1 antibody [EPR1390Y]

     Good
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Tissue lysate - whole (brain)
    Gel Running Conditions
    Reduced Denaturing (12)
    Loading amount
    50 µg
    Specification
    brain
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted May 13 2016

    Western blot abreview for Anti-Heme Oxygenase 1 antibody [EPR1390Y]

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    African Green Monkey Cell lysate - whole cell (COS-7 cells)
    Gel Running Conditions
    Reduced Denaturing (4-12% Bis-Tris)
    Loading amount
    100000 cells
    Treatment
    30 nM Bortezomib for 24 hours
    Specification
    COS-7 cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10%
    Read More

    Abcam user community

    Verified customer

    Submitted Jun 22 2015

    Western blot abreview for Anti-Heme Oxygenase 1 antibody [EPR1390Y]

     Good
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    40 µg
    Gel Running Conditions
    Reduced Denaturing (10 % gel)
    Sample
    Rat Tissue lysate - whole (aorta)
    Specification
    aorta
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More

    Abcam user community

    Verified customer

    Submitted Oct 22 2013

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com