For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Customized Products & Partnerships
    Customized Products & Partnerships

    Customized products and commercial partnerships to accelerate your diagnostic and therapeutic programs.

    Customized products

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

  1. Link

    heme-oxygenase-1-antibody-epr18161-128-ab189491.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Neuroscience Neurology process Neurodegenerative disease Alzheimer's disease Other
Share by email
RecombinantRabMAb

Recombinant Anti-Heme Oxygenase 1 antibody [EPR18161-128] (ab189491)

  • Datasheet
  • Certificate of Compliance
Reviews (1) Submit a question References (21)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Shipping info

Promotion Information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Heme Oxygenase 1 antibody [EPR18161-128] (ab189491)
  • Western blot - Anti-Heme Oxygenase 1 antibody [EPR18161-128] (ab189491)
  • Immunocytochemistry/ Immunofluorescence - Anti-Heme Oxygenase 1 antibody [EPR18161-128] (ab189491)
  • Immunocytochemistry/ Immunofluorescence - Anti-Heme Oxygenase 1 antibody [EPR18161-128] (ab189491)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Heme Oxygenase 1 antibody [EPR18161-128] (ab189491)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Heme Oxygenase 1 antibody [EPR18161-128] (ab189491)
  • Western blot - Anti-Heme Oxygenase 1 antibody [EPR18161-128] (ab189491)
  • Flow Cytometry (Intracellular) - Anti-Heme Oxygenase 1 antibody [EPR18161-128] (ab189491)
  • Flow Cytometry (Intracellular) - Anti-Heme Oxygenase 1 antibody [EPR18161-128] (ab189491)
  • Immunoprecipitation - Anti-Heme Oxygenase 1 antibody [EPR18161-128] (ab189491)
  • Anti-Heme Oxygenase 1 antibody [EPR18161-128] (ab189491)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR18161-128] to Heme Oxygenase 1
  • Suitable for: WB, IHC-P, IP, ICC/IF, Flow Cyt (Intra)
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 647 Carrier Free PE

You may also be interested in

Biochemical
Product image
IEM-1460, GluR2 lacking AMPA receptor blocker (ab141507)
ELISA
Product image
Human Heme Oxygenase 1 ELISA Kit, Fluorescent (ab229429)
Primary
Product image
Anti-Tau (phospho S202 + T205) antibody [EPR20390] - BSA and Azide free (ab237594)

View more associated products

Overview

  • Product name

    Anti-Heme Oxygenase 1 antibody [EPR18161-128]
    See all Heme Oxygenase 1 primary antibodies
  • Description

    Rabbit monoclonal [EPR18161-128] to Heme Oxygenase 1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, IP, ICC/IF, Flow Cyt (Intra)more details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human, mouse and rat spleen lysate. NIH/3T3 and HeLa cell lysate. IHC-P: Human, mouse and rat liver tissue. ICC/IF: HeLa and NIH/3T3 cells. Flow Cyt (intra): HeLa and NIH/3T3 cells. IP: NIH/3T3 whole cell lysate.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR18161-128
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Alzheimer's disease
    • Other
    • Cardiovascular
    • Blood
    • Platelets
    • Signal Transduction
    • Signaling Pathway
    • Nuclear Signaling
    • NFkB Pathway
    • Epigenetics and Nuclear Signaling
    • Nuclear Signaling Pathways
    • NFkB pathway
    • Cardiovascular
    • Atherosclerosis
    • Vascular Inflammation
    • Inflammatory mediators
    • Cardiovascular
    • Vasculature
    • Endothelium
    • Cancer
    • Cancer Metabolism
    • Response to hypoxia
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Hypoxia
    • Metabolism
    • Types of disease
    • Neurodegenerative disease
    • Metabolism
    • Types of disease
    • Heart disease

Associated products

  • Alternative Versions

    • Anti-Heme Oxygenase 1 antibody [EPR18161-128] - BSA and Azide free (ab224677)
    • Alexa Fluor® 488 Anti-Heme Oxygenase 1 antibody [EPR18161-128] (ab237267)
    • Alexa Fluor® 647 Anti-Heme Oxygenase 1 antibody [EPR18161-128] (ab237268)
    • PE Anti-Heme Oxygenase 1 antibody [EPR18161-128] (ab237269)
  • Compatible Secondaries

    • VeriBlot for IP Detection Reagent (HRP) (ab131366)
    • Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195889)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
  • Immunohistochemistry reagents

    • Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (ab93684)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human Heme Oxygenase 1 protein (ab85243)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab189491 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (1)
1/2000. Predicted molecular weight: 33 kDa.
IHC-P
1/20000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IP
1/30.
ICC/IF
1/250.
Flow Cyt (Intra)
1/50.
Notes
WB
1/2000. Predicted molecular weight: 33 kDa.
IHC-P
1/20000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IP
1/30.
ICC/IF
1/250.
Flow Cyt (Intra)
1/50.

Target

  • Function

    Heme oxygenase cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed.
  • Sequence similarities

    Belongs to the heme oxygenase family.
  • Cellular localization

    Microsome. Endoplasmic reticulum.
  • Target information above from: UniProt accession P09601 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 3162 Human
    • Entrez Gene: 15368 Mouse
    • Entrez Gene: 24451 Rat
    • Omim: 141250 Human
    • SwissProt: P09601 Human
    • SwissProt: P14901 Mouse
    • SwissProt: P06762 Rat
    • Unigene: 517581 Human
    • Unigene: 276389 Mouse
    • Unigene: 3160 Rat
    see all
  • Alternative names

    • 32 kD antibody
    • bK286B10 antibody
    • D8Wsu38e antibody
    • heat shock protein 32 kD antibody
    • heat shock protein 32kD antibody
    • Heat shock protein antibody
    • Heme oxygenase (decycling) 1 antibody
    • Heme oxygenase 1 antibody
    • Hemox antibody
    • HMOX 1 antibody
    • Hmox antibody
    • Hmox1 antibody
    • HMOX1_HUMAN antibody
    • HO 1 antibody
    • HO antibody
    • HO-1 antibody
    • HO1 antibody
    • Hsp32 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Heme Oxygenase 1 antibody [EPR18161-128] (ab189491)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Heme Oxygenase 1 antibody [EPR18161-128] (ab189491)

    Immunohistochemical analysis of paraffin embedded mouse liver tissue labeling Heme Oxygenase 1 with ab189491 at 1/20000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on Kupffer cells of mouse liver (PMID: 9449694) is observed. Counter stained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Western blot - Anti-Heme Oxygenase 1 antibody [EPR18161-128] (ab189491)
    Western blot - Anti-Heme Oxygenase 1 antibody [EPR18161-128] (ab189491)
    Anti-Heme Oxygenase 1 antibody [EPR18161-128] (ab189491) at 1/2000 dilution + Human spleen lysate at 10 µg

    Secondary
    VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/4000 dilution

    Predicted band size: 33 kDa
    Observed band size: 28, 32 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking: 5% NFDM/TBST.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 18400743).

    The lower band observed is a truncated form of Heme Oxygenase 1 (PMID: 17430897).

  • Immunocytochemistry/ Immunofluorescence - Anti-Heme Oxygenase 1 antibody [EPR18161-128] (ab189491)
    Immunocytochemistry/ Immunofluorescence - Anti-Heme Oxygenase 1 antibody [EPR18161-128] (ab189491)

    Immunofluorescent analysis of 4% paraformaldehyde fixed, 0.1% Triton X-100 permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cells labeling Heme Oxygenase 1 with ab189491 at 1/250 dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on HeLa cells. Details of counterstains: ab195889  Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at a 1/200 dilution; DAPI for nuclei.
    The negative controls are as follows: Secondary antibody only for control.

  • Immunocytochemistry/ Immunofluorescence - Anti-Heme Oxygenase 1 antibody [EPR18161-128] (ab189491)
    Immunocytochemistry/ Immunofluorescence - Anti-Heme Oxygenase 1 antibody [EPR18161-128] (ab189491)

    Immunofluorescent analysis of 4% paraformaldehyde fixed, 0.1% Triton X-100 permeabilized NIH/3T3 (mouse embryonic fibroblast) cells labeling Heme Oxygenase 1 with ab189491 at 1/250 dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on NIH/3T3 cells. Details of counterstains: ab195889  Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at a 1/200 dilution; DAPI for nuclei.
    The negative controls are as follows: Secondary antibody only for control.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Heme Oxygenase 1 antibody [EPR18161-128] (ab189491)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Heme Oxygenase 1 antibody [EPR18161-128] (ab189491)

    Immunohistochemical analysis of paraffin embedded human liver tissue labeling Heme Oxygenase 1 with ab189491 at 1/20,000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on Kupffer cells of human liver (PMID: 9449694) is observed. Counter stained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Heme Oxygenase 1 antibody [EPR18161-128] (ab189491)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Heme Oxygenase 1 antibody [EPR18161-128] (ab189491)

    Immunohistochemical analysis of paraffin embedded rat liver tissue labeling Heme Oxygenase 1 with ab189491 at 1/20,000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on Kupffer cells of rat liver (PMID: 9449694) is observed. Counter stained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Western blot - Anti-Heme Oxygenase 1 antibody [EPR18161-128] (ab189491)
    Western blot - Anti-Heme Oxygenase 1 antibody [EPR18161-128] (ab189491)
    All lanes : Anti-Heme Oxygenase 1 antibody [EPR18161-128] (ab189491) at 1/5000 dilution

    Lane 1 : NIH/3T3 (mouse embryonic fibroblast) lysate
    Lane 2 : Rat spleen lysate
    Lane 3 : HeLa (human cervix adenocarcinoma epithelial cell) lysate
    Lane 4 : Mouse spleen lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 33 kDa
    Observed band size: 28, 32 kDa why is the actual band size different from the predicted?



    Blocking: 5% NFDM/TBST.

    Exposure time:

    Lanes 1,2 and 3: 2 seconds;

    Lane 4: 1 second

    The molecular weight observed is consistent with what has been described in the literature (PMID: 18400743).

    The lower band observed is a truncated form of Heme Oxygenase 1 (PMID: 17430897).

  • Flow Cytometry (Intracellular) - Anti-Heme Oxygenase 1 antibody [EPR18161-128] (ab189491)
    Flow Cytometry (Intracellular) - Anti-Heme Oxygenase 1 antibody [EPR18161-128] (ab189491)

    Intracellular flow cytometric analysis of 90% methanol/ 4% paraformaldehyde fixed NIH/3T3 (mouse embryonic fibroblast) cell line labeling Heme Oxygenase1 with ab189491 at 1/50 (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluorr® 488, ab150077), at 1/2000 dilution was used as the secondary antibody. 

     

     

  • Flow Cytometry (Intracellular) - Anti-Heme Oxygenase 1 antibody [EPR18161-128] (ab189491)
    Flow Cytometry (Intracellular) - Anti-Heme Oxygenase 1 antibody [EPR18161-128] (ab189491)

    Intracellular flow cytometric analysis of 90% methanol/4% paraformaldehyde fixed HeLa (human cervix adenocarcinoma epithelial cell) cell line labeling Heme Oxygenase1 with ab189491 at 1/50 (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluorr® 488, ab150077), at 1/2000 dilution was used as the secondary antibody. 

     

     

  • Immunoprecipitation - Anti-Heme Oxygenase 1 antibody [EPR18161-128] (ab189491)
    Immunoprecipitation - Anti-Heme Oxygenase 1 antibody [EPR18161-128] (ab189491)

    Heme Oxygenase 1 was immunoprecipitated from 0.35 mg of NIH/3T3 (mouse embryonic fibroblast) whole cell lysate with ab189491 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab189491 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution.
    Lane 1: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate  10 μg (Input).
    Lane 2: NIH/3T3 whole cell lysate (+).
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of  ab189491 in NIH/3T3 whole cell lysate (-).
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    The truncated form of Heme Oxygenase 1 is described in the literature (PMID: 17430897).

  • Anti-Heme Oxygenase 1 antibody [EPR18161-128] (ab189491)
    Anti-Heme Oxygenase 1 antibody [EPR18161-128] (ab189491)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • Datasheet download

    Download

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

References (21)

Publishing research using ab189491? Please let us know so that we can cite the reference in this datasheet.

ab189491 has been referenced in 21 publications.

  • Yan H  et al. Activation of Akt-dependent Nrf2/ARE pathway by restoration of Brg-1 remits high glucose-induced oxidative stress and ECM accumulation in podocytes. J Biochem Mol Toxicol 35:e22672 (2021). PubMed: 33270355
  • Chen S  et al. Anti-inflammatory effect of salusin-ß knockdown on LPS-activated alveolar macrophages via NF-?B inhibition and HO-1 activation. Mol Med Rep 23:N/A (2021). PubMed: 33300078
  • Zhang L  et al. Isoflurane reduces septic neuron injury by HO-1-mediated abatement of inflammation and apoptosis. Mol Med Rep 23:N/A (2021). PubMed: 33355378
  • Rui Q  et al. LMTK2 regulates inflammation in lipopolysaccharide-stimulated BV2 cells. Exp Ther Med 21:219 (2021). PubMed: 33603828
  • Jia XB  et al. Lotus leaf flavonoids induce apoptosis of human lung cancer A549 cells through the ROS/p38 MAPK pathway. Biol Res 54:7 (2021). PubMed: 33653412
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

Western blot abreview for Anti-Heme Oxygenase 1 antibody [EPR18161-128]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (C166 cell line)
Gel Running Conditions
Non-reduced Denaturing (10%)
Loading amount
20 µg
Treatment
200uM H2O2
Specification
C166 cell line
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Read More

DR. Yue Zhao

Verified customer

Submitted Nov 03 2021

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Get resources and offers direct to your inbox Sign up
A-Z by research area
  • Cancer
  • Cardiovascular
  • Cell biology
  • Developmental biology
  • Epigenetics & Nuclear signaling
  • Immunology
  • Metabolism
  • Microbiology
  • Neuroscience
  • Signal transduction
  • Stem cells
A-Z by product type
  • Primary antibodies
  • Secondary antibodies
  • Biochemicals
  • Isotype controls
  • Flow cytometry multi-color selector
  • Kits
  • Loading controls
  • Lysates
  • Peptides
  • Proteins
  • Slides
  • Tags and cell markers
  • Tools & Reagents
Help & support
  • Support
  • Make an Inquiry
  • Protocols & troubleshooting
  • Placing an order
  • RabMAb products
  • Biochemical product FAQs
  • Training
  • Browse by Target
Company
  • Corporate site
  • Investor relations
  • Company news
  • Careers
  • About us
  • Blog
Events
  • Tradeshows
  • Conferences
International websites
  • abcam.cn
  • abcam.co.jp

Join with us

  • LinkedIn
  • facebook
  • Twitter
  • YouTube
  • Terms of sale
  • Website terms of use
  • Cookie policy
  • Privacy policy
  • Legal
  • Modern slavery statement
© 1998-2022 Abcam plc. All rights reserved.