Overview

  • Product name

    Anti-Heme-regulated inhibitor antibody
    See all Heme-regulated inhibitor primary antibodies
  • Description

    Rabbit polyclonal to Heme-regulated inhibitor
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human Heme-regulated inhibitor aa 304-601.

  • Positive control

    • HeLa and A375 whole cell lysates; A431 cells; H661 xenograft.
  • General notes

    Previously labelled as EIF2AK1. 

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 0.75% Glycine, 1.21% Tris, 10% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab154076 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 71 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Inhibits protein synthesis at the translation initiation level, in response to various stress conditions, including oxidative stress, heme deficiency, osmotic shock and heat shock. Exerts its function through the phosphorylation of EIF2S1 at 'Ser-48' and 'Ser-51', thus preventing its recycling. Binds hemin forming a 1:1 complex through a cysteine thiolate and histidine nitrogenous coordination. This binding occurs with moderate affinity, allowing it to sense the heme concentration within the cell. Thanks to this unique heme-sensing capacity, plays a crucial role to shut off protein synthesis during acute heme-deficient conditions. In red blood cells (RBCs), controls hemoglobin synthesis ensuring a coordinated regulation of the synthesis of its heme and globin moieties. Thus plays an essential protective role for RBC survival in anemias of iron deficiency. Similarly, in hepatocytes, involved in heme-mediated translational control of CYP2B and CYP3A and possibly other hepatic P450 cytochromes. May also contain ER stress during acute heme-deficient conditions.
  • Tissue specificity

    Expressed predominantly in erythroid cells. At much lower levels, expressed in hepatocytes (at protein level).
  • Sequence similarities

    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. GCN2 subfamily.
    Contains 2 HRM (heme regulatory motif) repeats.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications

    Activated by autophosphorylation; phosphorylated predominantly on serine and threonine residues, but also on tyrosine residues. Autophosphorylation at Thr-488 is required for kinase activation. The active autophosphorylated form apparently is largely refractory to cellular heme fluctuations.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • E2AK1_HUMAN antibody
    • EC 2.7.11.1 antibody
    • Eif2ak1 antibody
    • Eukaryotic translation initiation factor 2 alpha kinase 1 antibody
    • Eukaryotic translation initiation factor 2-alpha kinase 1 antibody
    • HCR antibody
    • Heme controlled repressor antibody
    • Heme regulated eukaryotic initiation factor eIF 2 alpha kinase antibody
    • Heme regulated inhibitor antibody
    • Heme regulated initiation factor 2 alpha kinase antibody
    • Heme regulated repressor antibody
    • Heme sensitive initiation factor 2a kinase antibody
    • Heme-controlled repressor antibody
    • Heme-regulated eukaryotic initiation factor eIF-2-alpha kinase antibody
    • Heme-regulated inhibitor antibody
    • Hemin sensitive initiation factor 2 alpha kinase antibody
    • Hemin-sensitive initiation factor 2-alpha kinase antibody
    • HRI antibody
    • KIAA1369 antibody
    • PRO1362 antibody
    see all

Images

  • All lanes : Anti-Heme-regulated inhibitor antibody (ab154076) at 1/1000 dilution

    Lane 1 : HeLa whole cell lysate
    Lane 2 : A375 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 71 kDa



    7.5 % SDS-PAGE
  • Immunofluorescent analysis of paraformaldehyde-fixed A431 cells labeling Heme-regulated inhibitor with ab154076 at 1/200 dilution. Lower panel merged with DNA probe.

  • Immunohistochemical analysis of paraffin-embedded H661 xenograft labeling Heme-regulated inhibitor with ab154076 at 1/500 dilution.

References

ab154076 has not yet been referenced specifically in any publications.

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