Key features and details
- Rabbit polyclonal to Heme-regulated inhibitor
- Suitable for: IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Heme-regulated inhibitor antibody
See all Heme-regulated inhibitor primary antibodies
DescriptionRabbit polyclonal to Heme-regulated inhibitor
Tested applicationsSuitable for: IPmore details
Species reactivityReacts with: Human
Synthetic peptide within Human Heme-regulated inhibitor aa 580-630. The exact sequence is proprietary. NP_055228.2
Database link: Q9BQI3
- IP: HeLa whole cell lysate.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab245591 was affinity purified using an epitope specific to Heme-regulated inhibitor immobilized on solid support.
Our Abpromise guarantee covers the use of ab245591 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 5-15 µg/mg of lysate.|
FunctionInhibits protein synthesis at the translation initiation level, in response to various stress conditions, including oxidative stress, heme deficiency, osmotic shock and heat shock. Exerts its function through the phosphorylation of EIF2S1 at 'Ser-48' and 'Ser-51', thus preventing its recycling. Binds hemin forming a 1:1 complex through a cysteine thiolate and histidine nitrogenous coordination. This binding occurs with moderate affinity, allowing it to sense the heme concentration within the cell. Thanks to this unique heme-sensing capacity, plays a crucial role to shut off protein synthesis during acute heme-deficient conditions. In red blood cells (RBCs), controls hemoglobin synthesis ensuring a coordinated regulation of the synthesis of its heme and globin moieties. Thus plays an essential protective role for RBC survival in anemias of iron deficiency. Similarly, in hepatocytes, involved in heme-mediated translational control of CYP2B and CYP3A and possibly other hepatic P450 cytochromes. May also contain ER stress during acute heme-deficient conditions.
Tissue specificityExpressed predominantly in erythroid cells. At much lower levels, expressed in hepatocytes (at protein level).
Sequence similaritiesBelongs to the protein kinase superfamily. Ser/Thr protein kinase family. GCN2 subfamily.
Contains 2 HRM (heme regulatory motif) repeats.
Contains 1 protein kinase domain.
modificationsActivated by autophosphorylation; phosphorylated predominantly on serine and threonine residues, but also on tyrosine residues. Autophosphorylation at Thr-488 is required for kinase activation. The active autophosphorylated form apparently is largely refractory to cellular heme fluctuations.
- Information by UniProt
- E2AK1_HUMAN antibody
- EC 188.8.131.52 antibody
- Eif2ak1 antibody
Heme-regulated inhibitor was immunoprecipitated from HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg per IP reaction; 20% of IP loaded).
ab245591 used for IP at 10 µg/mg lysate. For WB ab245590 was used at 1 µg/ml.
Lane 1: ab245591 IP in HeLa whole cell lysate.
Lane 2: Control IgG in HeLa whole cell lysate.
Chemiluminescence detection: 10 seconds.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab245591 has not yet been referenced specifically in any publications.