Overview

  • Product name

    Anti-Heme-regulated inhibitor antibody
    See all Heme-regulated inhibitor primary antibodies
  • Description

    Rabbit polyclonal to Heme-regulated inhibitor
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide corresponding to Human Heme-regulated inhibitor aa 107-156 (N terminal).
    Database link: NP_055228

  • Positive control

    • Fetal lung lysate.
  • General notes

    Previously labelled as EIF2AK1. 

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab84980 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 71 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use at an assay dependent concentration.

Target

  • Function

    Inhibits protein synthesis at the translation initiation level, in response to various stress conditions, including oxidative stress, heme deficiency, osmotic shock and heat shock. Exerts its function through the phosphorylation of EIF2S1 at 'Ser-48' and 'Ser-51', thus preventing its recycling. Binds hemin forming a 1:1 complex through a cysteine thiolate and histidine nitrogenous coordination. This binding occurs with moderate affinity, allowing it to sense the heme concentration within the cell. Thanks to this unique heme-sensing capacity, plays a crucial role to shut off protein synthesis during acute heme-deficient conditions. In red blood cells (RBCs), controls hemoglobin synthesis ensuring a coordinated regulation of the synthesis of its heme and globin moieties. Thus plays an essential protective role for RBC survival in anemias of iron deficiency. Similarly, in hepatocytes, involved in heme-mediated translational control of CYP2B and CYP3A and possibly other hepatic P450 cytochromes. May also contain ER stress during acute heme-deficient conditions.
  • Tissue specificity

    Expressed predominantly in erythroid cells. At much lower levels, expressed in hepatocytes (at protein level).
  • Sequence similarities

    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. GCN2 subfamily.
    Contains 2 HRM (heme regulatory motif) repeats.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications

    Activated by autophosphorylation; phosphorylated predominantly on serine and threonine residues, but also on tyrosine residues. Autophosphorylation at Thr-488 is required for kinase activation. The active autophosphorylated form apparently is largely refractory to cellular heme fluctuations.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • E2AK1_HUMAN antibody
    • EC 2.7.11.1 antibody
    • Eif2ak1 antibody
    • Eukaryotic translation initiation factor 2 alpha kinase 1 antibody
    • Eukaryotic translation initiation factor 2-alpha kinase 1 antibody
    • HCR antibody
    • Heme controlled repressor antibody
    • Heme regulated eukaryotic initiation factor eIF 2 alpha kinase antibody
    • Heme regulated inhibitor antibody
    • Heme regulated initiation factor 2 alpha kinase antibody
    • Heme regulated repressor antibody
    • Heme sensitive initiation factor 2a kinase antibody
    • Heme-controlled repressor antibody
    • Heme-regulated eukaryotic initiation factor eIF-2-alpha kinase antibody
    • Heme-regulated inhibitor antibody
    • Hemin sensitive initiation factor 2 alpha kinase antibody
    • Hemin-sensitive initiation factor 2-alpha kinase antibody
    • HRI antibody
    • KIAA1369 antibody
    • PRO1362 antibody
    see all

Images

  • ab84980 staining Heme-regulated inhibitor in lung tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

  • ab84980 staining Heme-regulated inhibitor in brain tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

References

This product has been referenced in:

  • Taniuchi S  et al. Integrated stress response of vertebrates is regulated by four eIF2a kinases. Sci Rep 6:32886 (2016). WB ; Mouse . Read more (PubMed: 27633668) »
See 1 Publication for this product

Customer reviews and Q&As

Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293T cell line)
Gel Running Conditions
Reduced Denaturing (7.5)
Loading amount
20 µg
Specification
HEK293T cell line
Blocking step
Blocking One as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C

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Verified customer

Submitted Feb 27 2015

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