Product nameAnti-Hemogen antibody
See all Hemogen primary antibodies
DescriptionRabbit polyclonal to Hemogen
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee
Synthetic peptide corresponding to Human Hemogen aa 1-100 conjugated to keyhole limpet haemocyanin.
(Peptide available as
- This antibody gave a positive signal in the following Human Lysates: Testis Tissue, Ovary Tissue, Pancreas Tissue, Lymph node Tissue, Brain Tissue (data not shown), Spleen Tissue (data not shown), Thymus Tissue (data not shown), HeLa Whole Cell (data not shown), SW480 Whole Cell (data not shown), HepG2 Whole Cell (data not shown)
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab66550 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 58 kDa (predicted molecular weight: 55 kDa).|
FunctionRegulates the proliferation and differentiation of hematopoietic cells. Overexpression block the TPA-induced megakaryocytic differentiation in the K562 cell model. May also prevent cell apoptosis through the activation of the nuclear factor-kappa B (NF-kB).
Tissue specificityExpressed in hematopoietic precursor cells (at protein level). Detected in CD34+ and K562 leukemia cell line (at protein level). Expressed in bone marrow, testis, thymus. Also expressed in thymus and thyroid tumors, non-Hodgkin lymphoma, various leukemia cell lines, peripheral blood mononuclear cells (PBMCs) and bone marrow mononuclear cells (BMMCs) of patients with leukemia.
Developmental stageExpressed in fetal liver, kidney and brain.
- Information by UniProt
- CT155 antibody
- EDAG antibody
- EDAG-1 antibody
All lanes : Anti-Hemogen antibody (ab66550) at 1 µg/ml
Lane 1 : Human testis tissue lysate - total protein (ab30257)
Lane 2 : Human ovary tissue lysate - total protein (ab30222)
Lane 3 : Human pancreas tissue lysate - total protein (ab29816)
Lane 4 : Human lymph node tissue lysate - total protein (ab29871)
Lysates/proteins at 10 µg per lane.
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 55 kDa
Observed band size: 58 kDa why is the actual band size different from the predicted?
Additional bands at: 75 kDa. We are unsure as to the identity of these extra bands.
ab66550 has not yet been referenced specifically in any publications.