Key features and details
- Mouse monoclonal [7E1F] to Hemoglobin
- Suitable for: ELISA, WB, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-Hemoglobin antibody [7E1F]
See all Hemoglobin primary antibodies
DescriptionMouse monoclonal [7E1F] to Hemoglobin
Tested applicationsSuitable for: ELISA, WB, Flow Cytmore details
Species reactivityReacts with: Human
- Human plasma.
This product was changed from ascites to tissue culture supernatant on 20th January 2020. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.03% Sodium azide
Constituents: 0.01% BSA, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, HEPES
Concentration information loading...
Light chain typekappa
Our Abpromise guarantee covers the use of ab77125 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Predicted molecular weight: 15 kDa.
This antibody can be used diluted 1:5000 (PMID: 28965917).
|Flow Cyt||Use at an assay dependent concentration.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
FunctionInvolved in oxygen transport from the lung to the various peripheral tissues.
Tissue specificityRed blood cells.
Involvement in diseaseDefects in HBA1/HBA2 may be a cause of Heinz body anemias (HEIBAN) [MIM:140700]. This is a form of non-spherocytic hemolytic anemia of Dacie type 1. After splenectomy, which has little benefit, basophilic inclusions called Heinz bodies are demonstrable in the erythrocytes. Before splenectomy, diffuse or punctate basophilia may be evident. Most of these cases are probably instances of hemoglobinopathy. The hemoglobin demonstrates heat lability. Heinz bodies are observed also with the Ivemark syndrome (asplenia with cardiovascular anomalies) and with glutathione peroxidase deficiency.
Defects in HBA1/HBA2 are the cause of alpha-thalassemia (A-THAL) [MIM:604131]. The thalassemias are the most common monogenic diseases and occur mostly in Mediterranean and Southeast Asian populations. The hallmark of alpha-thalassemia is an imbalance in globin-chain production in the adult HbA molecule. The level of alpha chain production can range from none to very nearly normal levels. Deletion of both copies of each of the two alpha-globin genes causes alpha(0)-thalassemia, also known as homozygous alpha thalassemia. Due to the complete absence of alpha chains, the predominant fetal hemoglobin is a tetramer of gamma-chains (Bart hemoglobin) that has essentially no oxygen carrying capacity. This causes oxygen starvation in the fetal tissues leading to prenatal lethality or early neonatal death. The loss of three alpha genes results in high levels of a tetramer of four beta chains (hemoglobin H), causing a severe and life-threatening anemia known as hemoglobin H disease. Untreated, most patients die in childhood or early adolescence. The loss of two alpha genes results in mild alpha-thalassemia, also known as heterozygous alpha-thalassemia. Affected individuals have small red cells and a mild anemia (microcytosis). If three of the four alpha-globin genes are functional, individuals are completely asymptomatic. Some rare forms of alpha-thalassemia are due to point mutations (non-deletional alpha-thalassemia). The thalassemic phenotype is due to unstable globin alpha chains that are rapidly catabolized prior to formation of the alpha-beta heterotetramers.
Note=Alpha(0)-thalassemia is associated with non-immune hydrops fetalis, a generalized edema of the fetus with fluid accumulation in the body cavities due to non-immune causes. Non-immune hydrops fetalis is not a diagnosis in itself but a symptom, a feature of many genetic disorders, and the end-stage of a wide variety of disorders.
Sequence similaritiesBelongs to the globin family.
modificationsThe initiator Met is not cleaved in variant Thionville and is acetylated.
- Information by UniProt
- 3-prime alpha-globin gene antibody
- A gamma globin antibody
- Alpha 1 globin antibody
All lanes : Anti-Hemoglobin antibody [7E1F] (ab77125) at 1/1000 dilution
Lane 1 : Hemoglobin isolated from Human
Lane 2 : Human plasma at 1 µl
Predicted band size: 15 kDa
This image was generated using the ascites version of the product.
Overlay histogram showing K562 cells stained with ab77125 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab77125, 1/50 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
This image was generated using the ascites version of the product.
ab77125 has been referenced in 3 publications.
- Wu Z et al. Alpha-synuclein alterations in red blood cells of peripheral blood after acute ischemic stroke. Int J Clin Exp Pathol 12:1757-1763 (2019). PubMed: 31933994
- Laurén E et al. Phospholipid composition of packed red blood cells and that of extracellular vesicles show a high resemblance and stability during storage. Biochim Biophys Acta Mol Cell Biol Lipids 1863:1-8 (2018). WB ; Human . PubMed: 28965917
- Yang W et al. Neuronal hemoglobin in mitochondria is reduced by forming a complex with a-synuclein in aging monkey brains. Oncotarget 7:7441-54 (2016). WB, ELISA . PubMed: 26824991