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    hemoglobin-antibody-haem1-ab401.pdf

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Cardiovascular Blood Blood Cell Antigens RBC Antigens
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Anti-Hemoglobin antibody [Haem1] (ab401)

  • Datasheet
  • SDS
Submit a review Q&A (7)References (2)

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Key features and details

  • Mouse monoclonal [Haem1] to Hemoglobin
  • Reacts with: Human
  • Isotype: IgG1

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Overview

  • Product name

    Anti-Hemoglobin antibody [Haem1]
    See all Hemoglobin primary antibodies
  • Description

    Mouse monoclonal [Haem1] to Hemoglobin
  • Host species

    Mouse
  • Specificity

    hHb - 100% hAlbumin - <0.01% hHaptoglobin - 0.4% hMyoglobin - <0.03% hTransferrin - <0.01% Cross-reactivity values have been calculated on weight/weight basis. They can vary because of the presence of antigen or related impurities in protein preparations used for cross reactivity assays. Affinity constant: 1x1010 l/mol human haemoglobin.
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Full length native protein (purified) (Human).

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 6.00
    Preservative: 0.095% Sodium azide
    Constituents: 0.335% PBS, 1.0878% Sodium citrate
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    Haem1
  • Myeloma

    unknown
  • Isotype

    IgG1
  • Light chain type

    unknown
  • Research areas

    • Cardiovascular
    • Blood
    • Blood Cell Antigens
    • RBC Antigens
    • Cardiovascular
    • Blood
    • Other
    • Kits/ Lysates/ Other
    • Kits
    • ELISA Kits
    • ELISA Kits
    • Blood cell antigens ELISA kits

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)

Target

  • Function

    Involved in oxygen transport from the lung to the various peripheral tissues.
  • Tissue specificity

    Red blood cells.
  • Involvement in disease

    Defects in HBA1/HBA2 may be a cause of Heinz body anemias (HEIBAN) [MIM:140700]. This is a form of non-spherocytic hemolytic anemia of Dacie type 1. After splenectomy, which has little benefit, basophilic inclusions called Heinz bodies are demonstrable in the erythrocytes. Before splenectomy, diffuse or punctate basophilia may be evident. Most of these cases are probably instances of hemoglobinopathy. The hemoglobin demonstrates heat lability. Heinz bodies are observed also with the Ivemark syndrome (asplenia with cardiovascular anomalies) and with glutathione peroxidase deficiency.
    Defects in HBA1/HBA2 are the cause of alpha-thalassemia (A-THAL) [MIM:604131]. The thalassemias are the most common monogenic diseases and occur mostly in Mediterranean and Southeast Asian populations. The hallmark of alpha-thalassemia is an imbalance in globin-chain production in the adult HbA molecule. The level of alpha chain production can range from none to very nearly normal levels. Deletion of both copies of each of the two alpha-globin genes causes alpha(0)-thalassemia, also known as homozygous alpha thalassemia. Due to the complete absence of alpha chains, the predominant fetal hemoglobin is a tetramer of gamma-chains (Bart hemoglobin) that has essentially no oxygen carrying capacity. This causes oxygen starvation in the fetal tissues leading to prenatal lethality or early neonatal death. The loss of three alpha genes results in high levels of a tetramer of four beta chains (hemoglobin H), causing a severe and life-threatening anemia known as hemoglobin H disease. Untreated, most patients die in childhood or early adolescence. The loss of two alpha genes results in mild alpha-thalassemia, also known as heterozygous alpha-thalassemia. Affected individuals have small red cells and a mild anemia (microcytosis). If three of the four alpha-globin genes are functional, individuals are completely asymptomatic. Some rare forms of alpha-thalassemia are due to point mutations (non-deletional alpha-thalassemia). The thalassemic phenotype is due to unstable globin alpha chains that are rapidly catabolized prior to formation of the alpha-beta heterotetramers.
    Note=Alpha(0)-thalassemia is associated with non-immune hydrops fetalis, a generalized edema of the fetus with fluid accumulation in the body cavities due to non-immune causes. Non-immune hydrops fetalis is not a diagnosis in itself but a symptom, a feature of many genetic disorders, and the end-stage of a wide variety of disorders.
  • Sequence similarities

    Belongs to the globin family.
  • Post-translational
    modifications

    The initiator Met is not cleaved in variant Thionville and is acetylated.
  • Target information above from: UniProt accession P69905 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 3039 Human
    • Entrez Gene: 3040 Human
    • Entrez Gene: 3043 Human
    • Entrez Gene: 3047 Human
    • Omim: 141800 Human
    • Omim: 141900 Human
    • Omim: 142200 Human
    • SwissProt: P68871 Human
    • SwissProt: P69891 Human
    • SwissProt: P69905 Human
    • Unigene: 449630 Human
    • Unigene: 523443 Human
    • Unigene: 654744 Human
    see all
  • Alternative names

    • 3-prime alpha-globin gene antibody
    • A gamma globin antibody
    • Alpha 1 globin antibody
    • Alpha 2 globin antibody
    • Alpha 2 globin chain antibody
    • Alpha globin antibody
    • alpha one globin antibody
    • alpha-1 globin antibody
    • Alpha-globin antibody
    • Beta globin antibody
    • CD113t C antibody
    • CD31 antibody
    • Delta globin antibody
    • Erythremia, beta-globin type, included antibody
    • Gamma 1 globin antibody
    • Gamma A hemoglobin antibody
    • Gamma globin antibody
    • Hb F Agamma antibody
    • HBA 1 antibody
    • HBA 2 antibody
    • HBA antibody
    • HBA T3 antibody
    • HBA-T2 antibody
    • HBA_HUMAN antibody
    • HBA1 antibody
    • HBA2 antibody
    • HBB antibody
    • Hbb-y antibody
    • HBD antibody
    • Hbe1 antibody
    • HBG 1 antibody
    • HBG antibody
    • HBG1 antibody
    • HBGA antibody
    • HBGR antibody
    • HBH antibody
    • Hemoglobin alpha 1 antibody
    • Hemoglobin alpha 1 chain antibody
    • hemoglobin alpha 1 globin chain antibody
    • hemoglobin alpha 2 antibody
    • Hemoglobin alpha chain antibody
    • Hemoglobin alpha locus antibody
    • Hemoglobin alpha locus 1 antibody
    • hemoglobin alpha-1 chain antibody
    • Hemoglobin beta antibody
    • Hemoglobin beta chain antibody
    • Hemoglobin beta chain complex antibody
    • Hemoglobin beta locus antibody
    • Hemoglobin gamma 1 chain antibody
    • Hemoglobin gamma A antibody
    • Hemoglobin gamma A chain antibody
    • Hemoglobin gamma antibody
    • Hemoglobin subunit alpha antibody
    • Hemoglobin subunit beta antibody
    • Hemoglobin subunit gamma 1 antibody
    • hemoglobin, gamma, regulator of antibody
    • Hemoglobin--gamma locus, 136 alanaine antibody
    • HSGGL1 antibody
    • LVV-hemorphin-7 antibody
    • Methemoglobinemia, beta-globin type, included antibody
    • MGC126895 antibody
    • MGC126897 antibody
    • Minor alpha-globin locus antibody
    • PRO2979 antibody
    see all

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (2)

Publishing research using ab401? Please let us know so that we can cite the reference in this datasheet.

ab401 has been referenced in 2 publications.

  • Nakazawa H  et al. Coenzyme Q10 protects against burn-induced mitochondrial dysfunction and impaired insulin signaling in mouse skeletal muscle. FEBS Open Bio 9:348-363 (2019). PubMed: 30761259
  • Liu DN  et al. Superior mesenteric artery margin in pancreaticoduodenectomy for pancreatic adenocarcinoma. Oncotarget 8:7766-7776 (2017). PubMed: 27999192

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-7 of 7 Abreviews or Q&A

Question

Yes,
I will also need to purchase the protein standard.
Thanks,

Read More

Abcam community

Verified customer

Asked on Mar 09 2012

Answer

Thank you for contacting us.

The positive control ab77858 https://www.abcam.com/Hemoglobin-protein-ab77858.html should work with ab401.

Please be advised we have not used these products together. Our assumption is only based on the nature of protein and the immunogen of ab401 raised against. You can buy these products without any hesitation.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More

Abcam Scientific Support

Answered on Mar 09 2012

Question

I’m looking for an anti-body for total hemoglobin (human reactivity), but I would like a rabbit polyclonal. Which one would you suggest? If you don’t have a rabbit still suggest one.

Thanks,

Read More

Abcam community

Verified customer

Asked on Mar 09 2012

Answer

Thank you for contacting us.

We do not have rabbit antibody in catalogue we however have mouse monoclonal anti Hemoglobin antibody; the catalogue number is ab401; https://www.abcam.com/Hemoglobin-antibody-Haem1-ab401.html

This antibody is fully guaranteed to be used in ELISA and RIA.

Let me know if you are also interested in purchasing the compatible products of this antibody.I can help finding the one.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More

Abcam Scientific Support

Answered on Mar 09 2012

Question

The customer asked if this antibody recognizes adult hemoglobin only (which she would like), or fetal hemoglobin also.

Read More

Abcam community

Verified customer

Asked on May 04 2005

Answer

Thank you for your patience in waiting for a response regarding ab401 and ab402. The source of these antibodies returned my email today and stated that in all likelihood antibodies ab401 and ab402 to human Hemoglobin do not recognize fetal Hb. They have not tested them in their laboratory, but some researchers have results indicating that they do not. Again, I am sorry about the delay and do hesitate to contact us if you have further questions,

Read More

Abcam Scientific Support

Answered on May 17 2005

Question

Customer would like to know for these 3 antibodies, ab9249, ab401, and ab402: do they recognize beta hemoglobin and where is the epitope? He in interested in using them for WB.

Read More

Abcam community

Verified customer

Asked on Dec 29 2003

Answer

Regarding both ab401 and ab402, the epitope has not been identified, but is thought to be in the beta chain. To our knowledge, these antibodies have yet to be tested in Western blotting. All tested applications are specified on Abcam product datasheets. If you decide to go ahead and purchase one of these products, please let us know how you get on and in return we will forward a reward of your choice, typically an Amazon gift voucher. When I receive information regarding ab9249, I will pass the information on to you.

Read More

Abcam Scientific Support

Answered on Dec 29 2003

Question

Dear Sirs, do you offer a ready-to-use anti-human hemoglobin ELISA? I am looking for a possibility to measure the hemoglobin content in cell culture models. Tank you for your information

Read More

Abcam community

Verified customer

Asked on Nov 13 2001

Answer

We stock a number of ELISA kits, but do not currently stock an anti-human hemoglobin ELISA kit.

Read More

Abcam Scientific Support

Answered on Nov 14 2001

Question

In smoke exposure, the blood shows increased levels of carboxyhemoglobin. Any information on antibodies specific for this modified version ?

Read More

Abcam community

Verified customer

Asked on Oct 19 2001

Answer

We believe that antibodies to this modified version of hemoglobin are not commercially available.

Read More

Abcam Scientific Support

Answered on Oct 19 2001

Question

Which is the epitope? alfa or beta chain?

Read More

Abcam community

Verified customer

Asked on Aug 24 2001

Answer

The epitope has not been identified for this antibody, but is thought to be in the beta chain.

Read More

Abcam Scientific Support

Answered on Aug 30 2001

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