Overview

  • Product name
    Anti-Hemoglobin antibody (HRP)
    See all Hemoglobin primary antibodies
  • Description
    Goat polyclonal to Hemoglobin (HRP)
  • Host species
    Goat
  • Conjugation
    HRP
  • Specificity
    The antibody has been tested in ELISA and IEP with a Human Hemaglobin Calibrator/Standard but has yet to be tested against endogenous protein.
  • Tested applications
    Suitable for: ICC, ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rabbit, Dog, Chimpanzee, Rhesus monkey
  • Immunogen

    Human Hemoglobin

  • Positive control
    • recombinant human hemoglobin
  • General notes

    Molar enzyme/antibody protein ratio is 4:1.

Properties

Applications

Our Abpromise guarantee covers the use of ab19362 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC
ELISA
WB
  • Application notes
    ELISA: 1/10000 - 1/100000.
    ICC: 1/200 - 1/500.
    WB: 1/1000 - 1/10000.
    Colorimetric detection: 1/1000 - 1/10000
    Chemiluminescent detection: 1/1000 - 1/30000
    Predicted molecular weight: 16 kDa (the protein is a tetramer; each subunit has a molecular weight of 16kDa).

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Involved in oxygen transport from the lung to the various peripheral tissues.
    • Tissue specificity
      Red blood cells.
    • Involvement in disease
      Defects in HBA1/HBA2 may be a cause of Heinz body anemias (HEIBAN) [MIM:140700]. This is a form of non-spherocytic hemolytic anemia of Dacie type 1. After splenectomy, which has little benefit, basophilic inclusions called Heinz bodies are demonstrable in the erythrocytes. Before splenectomy, diffuse or punctate basophilia may be evident. Most of these cases are probably instances of hemoglobinopathy. The hemoglobin demonstrates heat lability. Heinz bodies are observed also with the Ivemark syndrome (asplenia with cardiovascular anomalies) and with glutathione peroxidase deficiency.
      Defects in HBA1/HBA2 are the cause of alpha-thalassemia (A-THAL) [MIM:604131]. The thalassemias are the most common monogenic diseases and occur mostly in Mediterranean and Southeast Asian populations. The hallmark of alpha-thalassemia is an imbalance in globin-chain production in the adult HbA molecule. The level of alpha chain production can range from none to very nearly normal levels. Deletion of both copies of each of the two alpha-globin genes causes alpha(0)-thalassemia, also known as homozygous alpha thalassemia. Due to the complete absence of alpha chains, the predominant fetal hemoglobin is a tetramer of gamma-chains (Bart hemoglobin) that has essentially no oxygen carrying capacity. This causes oxygen starvation in the fetal tissues leading to prenatal lethality or early neonatal death. The loss of three alpha genes results in high levels of a tetramer of four beta chains (hemoglobin H), causing a severe and life-threatening anemia known as hemoglobin H disease. Untreated, most patients die in childhood or early adolescence. The loss of two alpha genes results in mild alpha-thalassemia, also known as heterozygous alpha-thalassemia. Affected individuals have small red cells and a mild anemia (microcytosis). If three of the four alpha-globin genes are functional, individuals are completely asymptomatic. Some rare forms of alpha-thalassemia are due to point mutations (non-deletional alpha-thalassemia). The thalassemic phenotype is due to unstable globin alpha chains that are rapidly catabolized prior to formation of the alpha-beta heterotetramers.
      Note=Alpha(0)-thalassemia is associated with non-immune hydrops fetalis, a generalized edema of the fetus with fluid accumulation in the body cavities due to non-immune causes. Non-immune hydrops fetalis is not a diagnosis in itself but a symptom, a feature of many genetic disorders, and the end-stage of a wide variety of disorders.
    • Sequence similarities
      Belongs to the globin family.
    • Post-translational
      modifications
      The initiator Met is not cleaved in variant Thionville and is acetylated.
    • Information by UniProt
    • Database links
    • Alternative names
      • 3-prime alpha-globin gene antibody
      • Alpha 2 globin chain antibody
      • Alpha globin antibody
      • alpha one globin antibody
      • alpha-1 globin antibody
      • Alpha-globin antibody
      • Beta globin antibody
      • CD113t C antibody
      • CD31 antibody
      • Delta globin antibody
      • Erythremia, beta-globin type, included antibody
      • Gamma 1 globin antibody
      • Hb F Agamma antibody
      • HBA 1 antibody
      • HBA 2 antibody
      • HBA antibody
      • HBA T3 antibody
      • HBA-T2 antibody
      • HBA_HUMAN antibody
      • HBA1 antibody
      • HBA2 antibody
      • HBB antibody
      • Hbb-y antibody
      • HBD antibody
      • Hbe1 antibody
      • HBG 1 antibody
      • HBG antibody
      • HBG1 antibody
      • HBGA antibody
      • HBGR antibody
      • HBH antibody
      • Hemoglobin alpha 1 antibody
      • hemoglobin alpha 1 globin chain antibody
      • Hemoglobin alpha chain antibody
      • Hemoglobin alpha locus antibody
      • Hemoglobin alpha locus 1 antibody
      • hemoglobin alpha-1 chain antibody
      • Hemoglobin beta antibody
      • Hemoglobin beta chain antibody
      • Hemoglobin beta chain complex antibody
      • Hemoglobin beta locus antibody
      • Hemoglobin gamma 1 chain antibody
      • Hemoglobin gamma A antibody
      • Hemoglobin gamma A chain antibody
      • Hemoglobin gamma antibody
      • Hemoglobin subunit alpha antibody
      • Hemoglobin subunit beta antibody
      • Hemoglobin subunit gamma 1 antibody
      • hemoglobin, gamma, regulator of antibody
      • Hemoglobin--gamma locus, 136 alanaine antibody
      • HSGGL1 antibody
      • LVV-hemorphin-7 antibody
      • Methemoglobinemia, beta-globin type, included antibody
      • MGC126895 antibody
      • MGC126897 antibody
      • Minor alpha-globin locus antibody
      • PRO2979 antibody
      see all

    References

    This product has been referenced in:
    • Potor L  et al. Hydrogen Sulfide Abrogates Hemoglobin-Lipid Interaction in Atherosclerotic Lesion. Oxid Med Cell Longev 2018:3812568 (2018). Read more (PubMed: 29560080) »
    See 1 Publication for this product

    Customer reviews and Q&As

    1-5 of 5 Abreviews or Q&A

    Question
    Answer

    Thank you for contacting Abcam regarding ab19362.


    I can confirm that this antibody will recognize the beta chain of hemoglobin.


    I hope this information is helpful. Please do not hesitate to contact me if you have any additional questions.

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    Answer

    I can confirm that the most common hemoglobin type is a tetramer (which contains 4 subunit proteins) called hemoglobin A, consisting of two a and two ß subunits non-covalently bound, each made of 141 and 146 amino acid residues, respectively. This is denoted as a2ß2. The subunits are structurally similar and about the same size. Each subunit has a molecular weight of about 16,000 daltons, for a total molecular weight of the tetramer of about 64,000 daltons. I have clarified this information on our datasheet and thank you for bringing this to my attention. Please do not hesitate to contact me if I can be of further assistance,

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    Answer

    Thank you for getting back to me. Unfortunately ab19192, ab19191, and ab19190 have only been shown to demonstrate reactivity against human so far. I do not have details of the predicted reactivity of these antibodies as unfortunately I do not have information as to the location of the epitope. I consider ab19362 your best option as this is predicted to react against rabbit and detects the alpha-globin monomer. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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    Answer

    Thank you for your enquiry. Yes, Hemoglobin antibody (ab19362) will detect the alpha-globin monomer. However, I have been informed by the lab that our sheep polyclonal antibodies ab19192, ab19191, and ab19190 (all derived from the same sera) all detect the alpha-globin monomer better than ab19362. You may wish to check out the datasheets of each of these antibodies; they are all conjugated to either HRP, AP or FITC. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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    Question
    Answer

    I would like to confirm that ab19363 is not the conjugated version of ab19362 ,the two antibodies are very different and unfortunately we do not have an non-HRP version of ab19362, my apologies. Should you decide to go ahead and purchase these products, please let us know how you get on by submitting a review and in return we will offer you 50 Abcam Loyalty points which you can use to redeem a variety of gifts. Please do not hesitate to contact us again if you need further assistance,

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