Key features and details
- Rabbit polyclonal to Hemoglobin subunit alpha
- Suitable for: IHC-P, WB
- Reacts with: Rat, Human
- Isotype: IgG
Product nameAnti-Hemoglobin subunit alpha antibody
See all Hemoglobin subunit alpha primary antibodies
DescriptionRabbit polyclonal to Hemoglobin subunit alpha
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Rat, Human
Predicted to work with: Chimpanzee, Monkey, Baboon, Macaque monkey, Rhesus monkey, Gorilla, Dolphin, Orangutan
Synthetic peptide corresponding to Human Hemoglobin subunit alpha aa 100 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available as
- This antibody gave a positive signal in human bone marrow and spleen tissue lysates.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab82871 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|WB||1/250. Detects a band of approximately 15 kDa (predicted molecular weight: 15 kDa).|
FunctionInvolved in oxygen transport from the lung to the various peripheral tissues.
Tissue specificityRed blood cells.
Involvement in diseaseHeinz body anemias
Alpha(0)-thalassemia is associated with non-immune hydrops fetalis, a generalized edema of the fetus with fluid accumulation in the body cavities due to non-immune causes. Non-immune hydrops fetalis is not a diagnosis in itself but a symptom, a feature of many genetic disorders, and the end-stage of a wide variety of disorders.
Hemoglobin H disease
Sequence similaritiesBelongs to the globin family.
modificationsThe initiator Met is not cleaved in variant Thionville and is acetylated.
- Information by UniProt
- Alpha 1 globin antibody
- Alpha globin antibody
- Alpha one globin antibody
All lanes : Anti-Hemoglobin subunit alpha antibody (ab82871) at 1/250 dilution
Lane 1 : Bone Marrow (Human) Tissue Lysate - adult normal tissue
Lane 2 : Human spleen tissue lysate - total protein (ab29699)
Lysates/proteins at 10 µg per lane.
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 15 kDa
Observed band size: 15 kDa
Additional bands at: 31 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds
IHC image of Hemoglobin A1c staining in human bone marrow formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab82871, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
ab17976 staining Hemoglobin A1c in Rat Lung tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% BSA in PBS for 20 minutes at 25°C; antigen retrieval was by heat mediation in a citrate buffer pH6.0. Samples were incubated with primary antibody (1/100 in blocking buffer) for 1 hours at 25°C. An undiluted HRP-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody
ab82871 has been referenced in 1 publication.
- Præstmark KA et al. Skin blood perfusion and cellular response to insertion of insulin pen needles with different diameters. J Diabetes Sci Technol 8:752-9 (2014). PubMed: 24876418