Anti-Hemoglobin subunit alpha antibody [EP3607] (ab109444)


  • Product name
    Anti-Hemoglobin subunit alpha antibody [EP3607]
    See all Hemoglobin subunit alpha primary antibodies
  • Description
    Rabbit monoclonal [EP3607] to Hemoglobin subunit alpha
  • Host species
  • Specificity
    Mouse and rat tissues tested (lung and kidney) gave negative or low sensitivity staining in IHC-P. This antibody may not be suitable for IHC with mouse or rat samples. If you have any questions, please contact our Scientific Support team.
  • Tested applications
    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Hemoglobin subunit alpha aa 1-100. The exact sequence is proprietary.

  • Positive control
    • WB: K562,fetal liver, and fetal heart lysates IHC-P: Human spleen and Human colon tissues
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents


  • Form
  • Storage instructions
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer
    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
  • Purity
    Tissue culture supernatant
  • Clonality
  • Clone number
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab109444 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 14 kDa (predicted molecular weight: 15 kDa).
IHC-P 1/250 - 1/500. Antigen retrieval is recommended.
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IP.
  • Target

    • Function
      Involved in oxygen transport from the lung to the various peripheral tissues.
    • Tissue specificity
      Red blood cells.
    • Involvement in disease
      Heinz body anemias
      Alpha(0)-thalassemia is associated with non-immune hydrops fetalis, a generalized edema of the fetus with fluid accumulation in the body cavities due to non-immune causes. Non-immune hydrops fetalis is not a diagnosis in itself but a symptom, a feature of many genetic disorders, and the end-stage of a wide variety of disorders.
      Hemoglobin H disease
    • Sequence similarities
      Belongs to the globin family.
    • Post-translational
      The initiator Met is not cleaved in variant Thionville and is acetylated.
    • Information by UniProt
    • Database links
    • Alternative names
      • Alpha 1 globin antibody
      • Alpha globin antibody
      • Alpha one globin antibody
      • Alpha-globin antibody
      • HBA_HUMAN antibody
      • HBA1 antibody
      • HBA2 antibody
      • Hemoglobin alpha 1 antibody
      • Hemoglobin alpha 1 chain antibody
      • Hemoglobin alpha 1 globin chain antibody
      • Hemoglobin alpha 2 antibody
      • Hemoglobin alpha chain antibody
      • Hemoglobin subunit alpha antibody
      • MGC126895 antibody
      • MGC126897 antibody
      see all


    • All lanes : Anti-Hemoglobin subunit alpha antibody [EP3607] (ab109444) at 1/1000 dilution

      Lane 1 : K562 lysate
      Lane 2 : Fetal liver lysate
      Lane 3 : Fetal heart lysates

      Lysates/proteins at 10 µg per lane.

      All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 15 kDa
      Observed band size: 14 kDa (why is the actual band size different from the predicted?)

    • Immunohistochemical analysis of Hemoglobin subunit alpha in paraffin embedded Human spleen tissue, using ab109444 at a 1/250 dilution.
    • Immunohistochemical analysis of Hemoglobin subunit alpha in paraffin embedded Human colon tissue, using ab109444 at a 1/250 dilution.


    ab109444 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Vielen Dank für Ihren Anruf.

    Es tut mir leid, dass Sie Probleme mit diesem Antikörper hatten. Wie am Telefon besprochen habe ich eine kostenlose Ersatzlieferung für Sie in Auftrag gegeben. Sie hat die Referenznummer 123456und so...

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    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

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    Thank you for your reply and for testing the antibodies.

    Unfortunately, we do not have another lot in stock for ab109444 - just the same lot. Please let me know if one of the other Anti-Hemoglobin subunit alpha antibodies we carry, ab92492 o...

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    According to our records, ab6721 or ab109444 was proving difficult to use in WBand we were in contact in order to help resolve the issue.

    Looking at our correspondence, it appears that we are awaiting more details in order to help us better ...

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    Thank you for contacting us.
    I am sorry to hear that one of these antibodies is notworking properly.

    As we discussed over the phone, trying a no-primary control (with secondary antibody only) would be another way to check if the problem...

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    Thank you for the reply.

    The recommended dilutions on our datasheets provide a concentration to begin with, however the optimal dilution does have to be determined by the end user. Therefore I strongly recommend that you try various concentra...

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    Thank you for sending that protocol information.

    If I have read the information correctly, then there are a few protocol variations that may work:

    1 - Did the customer only try a 1/5000 primary antibody dilution? If so, I strongly s...

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    I am sorry that the replacement antibody did not prove to succesful. Unfortunately the protocol that you attached did not come through. Could you resend the protocol so that I can look it over and see if there are any suggestions that I can make.

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