Anti-Hemoglobin subunit zeta + Hemoglobin subunit alpha antibody [EP3607] (ab109444)
Key features and details
- Rabbit monoclonal [EP3607] to Hemoglobin subunit zeta + Hemoglobin subunit alpha
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Hemoglobin subunit zeta + Hemoglobin subunit alpha antibody [EP3607] -
Description
Rabbit monoclonal [EP3607] to Hemoglobin subunit zeta + Hemoglobin subunit alpha -
Host species
Rabbit -
Specificity
Mouse and rat tissues tested (lung and kidney) gave negative or low sensitivity staining in IHC-P. This antibody may not be suitable for IHC with mouse or rat samples. If you have any questions, please contact our Scientific Support team.
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Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC/IF or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: K562, fetal liver, and fetal heart lysate. His-tagged full length human Hemoglobin subunit alpha and zeta recombinant protein. IHC-P: Human spleen and colon tissue.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP3607 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab109444 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Detects a band of approximately 14 kDa (predicted molecular weight: 15 kDa).
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IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Antigen retrieval is recommended. |
Notes |
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WB
1/1000 - 1/10000. Detects a band of approximately 14 kDa (predicted molecular weight: 15 kDa). |
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval is recommended. |
Images
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All lanes : Anti-Hemoglobin subunit zeta + Hemoglobin subunit alpha antibody [EP3607] (ab109444) at 1/1000 dilution
Lane 1 : His-tagged full length human Hemoglobin subunit alpha recombinant protein(ab131697)
Lane 2 : His-tagged human Hemoglobin subunit beta/ba1 (aa3-147) recombinant protein
Lane 3 : His-tagged human Hemoglobin subunit delta (aa3-147) recombinant protein
Lane 4 : His-tagged human Hemoglobin subunit epsilon (aa3-147) recombinant protein
Lane 5 : His-tagged human Hemoglobin subunit Gamma-2 (aa2-147) recombinant protein
Lane 6 : His-tagged human Hemoglobin subunit Gamma-1 (aa2-147) recombinant protein
Lane 7 : His-tagged full length human Hemoglobin subunit Zeta recombinant protein(ab95347)
Lysates/proteins at 0.01 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 15 kDa
Additional bands at: 14 kDa, 20 kDa. We are unsure as to the identity of these extra bands. -
All lanes : Anti-Hemoglobin subunit zeta + Hemoglobin subunit alpha antibody [EP3607] (ab109444) at 1/1000 dilution
Lane 1 : K562 lysate
Lane 2 : Fetal liver lysate
Lane 3 : Fetal heart lysates
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 15 kDa
Observed band size: 14 kDa why is the actual band size different from the predicted? -
Immunohistochemical analysis of Hemoglobin subunit alpha and Hemoglobin subunit zeta in paraffin embedded human spleen tissue, using ab109444 at a 1/250 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of Hemoglobin subunit alpha and Hemoglobin subunit zeta in paraffin embedded human colon tissue, using ab109444 at a 1/250 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab109444 has not yet been referenced specifically in any publications.