For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome
If you continue without changing your cookie settings, we'll assume you’re happy with this.
There is the researcher results.
Asked on Feb 08 2012
Thank you for sending that protocol information.
If I have read the information correctly, then there are a few protocol variations that may work:
1 - Did the customer only try a 1/5000 primary antibody dilution? If so, I strongly suggest that they try range of dilutions from 1/500-1/2000, to see if they can find the concentration that will work for them.
2 - According to the protocol, they loaded 5ug of protein on the gel. Did they try increasing the amount of protein, to say 20ug?
3 - How long did they incubate the primary antibody for? We recommend overnight at 4C.
4 - Did they try using 5% milk as a blocking reagent instead of BSA?
If the customer has not tried the protocol variations above, then I would strongly suggest that they do. If they have already tried those variations, then I would be happy to process a refund for this antibody, as it is not working as we guarantee.
I look forward to your reply.
Answered on Feb 09 2012