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2nd: 1/5000, 1/7000 high background
sees precipitate at bottom
trying right now same primary with other secondary
suggested to try no-primary crtl to check if problem comes from primary or secondary Ab
Asked on May 02 2012
Thank you for contacting us.
I am sorry to hear that one of these antibodies is notworking properly.
As we discussed over the phone, trying a no-primary control (with secondary antibody only) would be another way to check if the problem comes from the primary or secondary antibody.
I look forard to hear back from and assist you in resolving this problem.
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Answered on May 02 2012