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Thanks for your email reminder.Posdoc inthe labinvestigated both the antibodies (see attached pdf) using dot blot assay. Bottom-line, the primary antibody is not working fine.
We would appreciate if you couldsend usanother vial of the primary antibody from another batch/stock.
Asked on May 29 2012
Thank you for your reply and for testing the antibodies.
Unfortunately, we do not have another lot in stock for ab109444 - just the same lot. Please let me know if one of the other Anti-Hemoglobin subunit alpha antibodies we carry, ab92492 or ab102758, would be a suitable replacement.
Also, please let me know the species of the protein you tested (human, mouse or rat).
Originally, when you had called, the antibodies gave you high background according to our records, but from the dot blot you sent it looks now more like a weak signal/no staining.
Could you please let me know what samples were used for the original WB and what primary antibody dilution was used then? Also, please confirm if indeed high background was the issue.
We would need this information to update our records as we evaluatethe complaints we receive.
I look forward to hear back from you and resolve this case for you.
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Answered on May 29 2012