Anti-Hemoglobin subunit alpha antibody [EP3607] - BSA and Azide free (ab269881)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hemoglobin subunit alpha antibody [EP3607] - BSA and Azide free (ab269881)](https://www.abcam.com/ps/products/269/ab269881/Images/ab269881-368880-AntiHemoglobin-subunit-alpha-antibody-EP3607-immunohistochemistry-spleen-human.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hemoglobin subunit alpha antibody [EP3607] - BSA and Azide free (ab269881)")
Key features and details
- Rabbit monoclonal [EP3607] to Hemoglobin subunit alpha - BSA and Azide free
- Suitable for: WB, IHC-P
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Hemoglobin subunit alpha antibody [EP3607] - BSA and Azide free
See all Hemoglobin subunit alpha primary antibodies -
Description
Rabbit monoclonal [EP3607] to Hemoglobin subunit alpha - BSA and Azide free -
Host species
Rabbit -
Specificity
Mouse and rat tissues tested (lung and kidney) gave negative or low sensitivity staining in IHC-P. This antibody may not be suitable for IHC with mouse or rat samples. If you have any questions, please contact our Scientific Support team.
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Tested Applications & Species
See all applications and species dataApplication Species IHC-P Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: K562, fetal liver, and fetal heart lysate. IHC-P: Human spleen and colon tissue.
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General notes
ab269881 is the carrier-free version of ab109444. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EP3607 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab269881 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| IHC-P |
Human
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| All applications |
Mouse
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| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use at an assay dependent concentration. Detects a band of approximately 14 kDa (predicted molecular weight: 15 kDa).
|
|
| IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Antigen retrieval is recommended. |
| Notes |
|---|
|
WB
Use at an assay dependent concentration. Detects a band of approximately 14 kDa (predicted molecular weight: 15 kDa). |
|
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval is recommended. |
Target
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Function
Involved in oxygen transport from the lung to the various peripheral tissues. -
Tissue specificity
Red blood cells. -
Involvement in disease
Heinz body anemias
Alpha-thalassemia
Alpha(0)-thalassemia is associated with non-immune hydrops fetalis, a generalized edema of the fetus with fluid accumulation in the body cavities due to non-immune causes. Non-immune hydrops fetalis is not a diagnosis in itself but a symptom, a feature of many genetic disorders, and the end-stage of a wide variety of disorders.
Hemoglobin H disease -
Sequence similarities
Belongs to the globin family. -
Post-translational
modificationsThe initiator Met is not cleaved in variant Thionville and is acetylated. - Information by UniProt
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Database links
- Entrez Gene: 3039 Human
- Entrez Gene: 3040 Human
- Omim: 141800 Human
- Omim: 141850 Human
- SwissProt: P69905 Human
- SwissProt: P01942 Mouse
- SwissProt: P01946 Rat
- Unigene: 449630 Human
see all -
Alternative names
- Alpha 1 globin antibody
- Alpha globin antibody
- Alpha one globin antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hemoglobin subunit alpha antibody [EP3607] - BSA and Azide free (ab269881)
Immunohistochemical analysis of Hemoglobin subunit alpha in paraffin embedded human spleen tissue, using ab109444 at a 1/250 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
This image was produced using the same antibody clone but in a different formulation containing Tris glycine, sodium azide, glycerol and BSA (ab109444).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hemoglobin subunit alpha antibody [EP3607] - BSA and Azide free (ab269881)](https://www.abcam.com/ps/products/269/ab269881/Images/ab269881-368881-AntiHemoglobin-subunit-alpha-antibody-EP3607-immunohistochemistry-colon-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hemoglobin subunit alpha antibody [EP3607] - BSA and Azide free (ab269881)Immunohistochemical analysis of Hemoglobin subunit alpha in paraffin embedded human colon tissue, using ab109444 at a 1/250 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
This image was produced using the same antibody clone but in a different formulation containing Tris glycine, sodium azide, glycerol and BSA (ab109444).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab269881 has not yet been referenced specifically in any publications.