Anti-Hemoglobin subunit alpha antibody [EP3607] - BSA and Azide free (ab269881)
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- References
- Protocols
Overview
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Product name
Anti-Hemoglobin subunit alpha antibody [EP3607] - BSA and Azide free
See all Hemoglobin subunit alpha primary antibodies -
Description
Rabbit monoclonal [EP3607] to Hemoglobin subunit alpha - BSA and Azide free -
Host species
Rabbit -
Specificity
Mouse and rat tissues tested (lung and kidney) gave negative or low sensitivity staining in IHC-P. This antibody may not be suitable for IHC with mouse or rat samples. If you have any questions, please contact our Scientific Support team.
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Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human Hemoglobin subunit alpha aa 1-100. The exact sequence is proprietary.
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Positive control
- WB: K562, fetal liver, and fetal heart lysate. IHC-P: Human spleen and colon tissue.
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General notes
ab269881 is the carrier-free version of ab109444. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab269881 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.??
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: PBS -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EP3607 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab269881 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use at an assay dependent concentration. Detects a band of approximately 14 kDa (predicted molecular weight: 15 kDa). | |
| IHC-P | Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval is recommended. |
Target
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Function
Involved in oxygen transport from the lung to the various peripheral tissues. -
Tissue specificity
Red blood cells. -
Involvement in disease
Heinz body anemias
Alpha-thalassemia
Alpha(0)-thalassemia is associated with non-immune hydrops fetalis, a generalized edema of the fetus with fluid accumulation in the body cavities due to non-immune causes. Non-immune hydrops fetalis is not a diagnosis in itself but a symptom, a feature of many genetic disorders, and the end-stage of a wide variety of disorders.
Hemoglobin H disease -
Sequence similarities
Belongs to the globin family. -
Post-translational
modificationsThe initiator Met is not cleaved in variant Thionville and is acetylated. - Information by UniProt
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Database links
- Entrez Gene: 3039 Human
- Entrez Gene: 3040 Human
- Omim: 141800 Human
- Omim: 141850 Human
- SwissProt: P69905 Human
- SwissProt: P01942 Mouse
- SwissProt: P01946 Rat
- Unigene: 449630 Human
see all -
Alternative names
- Alpha 1 globin antibody
- Alpha globin antibody
- Alpha one globin antibody
see all
Images
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hemoglobin subunit alpha antibody [EP3607] - BSA and Azide free (ab269881)](https://www.abcam.com/ps/products/269/ab269881/Images/ab269881-368880-AntiHemoglobin-subunit-alpha-antibody-EP3607-immunohistochemistry-spleen-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hemoglobin subunit alpha antibody [EP3607] - BSA and Azide free (ab269881)Immunohistochemical analysis of Hemoglobin subunit alpha in paraffin embedded human spleen tissue, using ab109444 at a 1/250 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
This image was produced using the same antibody clone but in a different formulation containing Tris glycine, sodium azide, glycerol and BSA (ab109444).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hemoglobin subunit alpha antibody [EP3607] - BSA and Azide free (ab269881)](https://www.abcam.com/ps/products/269/ab269881/Images/ab269881-368881-AntiHemoglobin-subunit-alpha-antibody-EP3607-immunohistochemistry-colon-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hemoglobin subunit alpha antibody [EP3607] - BSA and Azide free (ab269881)Immunohistochemical analysis of Hemoglobin subunit alpha in paraffin embedded human colon tissue, using ab109444 at a 1/250 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
This image was produced using the same antibody clone but in a different formulation containing Tris glycine, sodium azide, glycerol and BSA (ab109444).
Datasheets and documents
References
ab269881 has not yet been referenced specifically in any publications.
