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Cardiovascular Blood Serum Proteins
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RabMAb

Anti-Hemoglobin subunit alpha antibody [EP3607] - BSA and Azide free (ab269881)

  • Datasheet
  • SDS
  • Certificate of Compliance
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hemoglobin subunit alpha antibody [EP3607] - BSA and Azide free (ab269881)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hemoglobin subunit alpha antibody [EP3607] - BSA and Azide free (ab269881)

Key features and details

  • Rabbit monoclonal [EP3607] to Hemoglobin subunit alpha - BSA and Azide free
  • Suitable for: WB, IHC-P
  • Reacts with: Rat, Human
  • Isotype: IgG

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Recombinant Human Hemoglobin subunit alpha protein (ab158638)

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Overview

  • Product name

    Anti-Hemoglobin subunit alpha antibody [EP3607] - BSA and Azide free
    See all Hemoglobin subunit alpha primary antibodies
  • Description

    Rabbit monoclonal [EP3607] to Hemoglobin subunit alpha - BSA and Azide free
  • Host species

    Rabbit
  • Specificity

    Mouse and rat tissues tested (lung and kidney) gave negative or low sensitivity staining in IHC-P. This antibody may not be suitable for IHC with mouse or rat samples. If you have any questions, please contact our Scientific Support team.

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: K562, fetal liver, and fetal heart lysate. IHC-P: Human spleen and colon tissue.
  • General notes

    ab269881 is the carrier-free version of ab109444. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Affinity purified
  • Clonality

    Monoclonal
  • Clone number

    EP3607
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Blood
    • Serum Proteins
    • Cardiovascular
    • Blood
    • Other

Associated products

  • Alternative Versions

    • Anti-Hemoglobin subunit alpha antibody [EP3607] (ab109444)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human Hemoglobin subunit alpha protein (ab158638)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab269881 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IHC-P
Human
All applications
Mouse
Application Abreviews Notes
WB
Use at an assay dependent concentration. Detects a band of approximately 14 kDa (predicted molecular weight: 15 kDa).
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Antigen retrieval is recommended.

Notes
WB
Use at an assay dependent concentration. Detects a band of approximately 14 kDa (predicted molecular weight: 15 kDa).
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Antigen retrieval is recommended.

  • Application notes
    Is unsuitable for Flow Cyt,ICC or IP.
  • Target

    • Function

      Involved in oxygen transport from the lung to the various peripheral tissues.
    • Tissue specificity

      Red blood cells.
    • Involvement in disease

      Heinz body anemias
      Alpha-thalassemia
      Alpha(0)-thalassemia is associated with non-immune hydrops fetalis, a generalized edema of the fetus with fluid accumulation in the body cavities due to non-immune causes. Non-immune hydrops fetalis is not a diagnosis in itself but a symptom, a feature of many genetic disorders, and the end-stage of a wide variety of disorders.
      Hemoglobin H disease
    • Sequence similarities

      Belongs to the globin family.
    • Post-translational
      modifications

      The initiator Met is not cleaved in variant Thionville and is acetylated.
    • Target information above from: UniProt accession P69905 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 3039 Human
      • Entrez Gene: 3040 Human
      • Omim: 141800 Human
      • Omim: 141850 Human
      • SwissProt: P69905 Human
      • SwissProt: P01942 Mouse
      • SwissProt: P01946 Rat
      • Unigene: 449630 Human
      • Unigene: 654744 Human
      • Unigene: 196110 Mouse
      • Unigene: 459653 Mouse
      see all
    • Alternative names

      • Alpha 1 globin antibody
      • Alpha globin antibody
      • Alpha one globin antibody
      • Alpha-globin antibody
      • HBA_HUMAN antibody
      • HBA1 antibody
      • HBA2 antibody
      • Hemoglobin alpha 1 antibody
      • Hemoglobin alpha 1 chain antibody
      • Hemoglobin alpha 1 globin chain antibody
      • Hemoglobin alpha 2 antibody
      • Hemoglobin alpha chain antibody
      • Hemoglobin subunit alpha antibody
      • MGC126895 antibody
      • MGC126897 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hemoglobin subunit alpha antibody [EP3607] - BSA and Azide free (ab269881)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hemoglobin subunit alpha antibody [EP3607] - BSA and Azide free (ab269881)

      Immunohistochemical analysis of Hemoglobin subunit alpha in paraffin embedded human spleen tissue, using ab109444 at a 1/250 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      This image was produced using the same antibody clone but in a different formulation containing Tris glycine, sodium azide, glycerol and BSA (ab109444).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hemoglobin subunit alpha antibody [EP3607] - BSA and Azide free (ab269881)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hemoglobin subunit alpha antibody [EP3607] - BSA and Azide free (ab269881)

      Immunohistochemical analysis of Hemoglobin subunit alpha in paraffin embedded human colon tissue, using ab109444 at a 1/250 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      This image was produced using the same antibody clone but in a different formulation containing Tris glycine, sodium azide, glycerol and BSA (ab109444).

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

    References (0)

    Publishing research using ab269881? Please let us know so that we can cite the reference in this datasheet.

    ab269881 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please use the links above to contact us or submit feedback about this product.

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