Recombinant
RabMAb

Anti-Hemoglobin subunit alpha antibody [EPR3608] - BSA and Azide free (ab174536)

Overview

  • Product name
    Anti-Hemoglobin subunit alpha antibody [EPR3608] - BSA and Azide free
    See all Hemoglobin subunit alpha primary antibodies
  • Description
    Rabbit monoclonal [EPR3608] to Hemoglobin subunit alpha - BSA and Azide free
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ELISA, Flow Cyt, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human Hemoglobin subunit alpha aa 50-150.

  • General notes

    ab174536 is a PBS-only buffer formulated version of ab92492, containing no BSA or sodium azide, ideal for antibody labeling. Please refer to ab92492 for information on protocols, dilutions, and image data.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Constituent: PBS
  • Concentration information loading...
  • Purity
    Affinity purified
  • Clonality
    Monoclonal
  • Clone number
    EPR3608
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab174536 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 14 kDa (predicted molecular weight: 15 kDa).

Can be blocked with ab206105

ELISA Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IP Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

Target

  • Function
    Involved in oxygen transport from the lung to the various peripheral tissues.
  • Tissue specificity
    Red blood cells.
  • Involvement in disease
    Heinz body anemias
    Alpha-thalassemia
    Alpha(0)-thalassemia is associated with non-immune hydrops fetalis, a generalized edema of the fetus with fluid accumulation in the body cavities due to non-immune causes. Non-immune hydrops fetalis is not a diagnosis in itself but a symptom, a feature of many genetic disorders, and the end-stage of a wide variety of disorders.
    Hemoglobin H disease
  • Sequence similarities
    Belongs to the globin family.
  • Post-translational
    modifications
    The initiator Met is not cleaved in variant Thionville and is acetylated.
  • Information by UniProt
  • Database links
  • Alternative names
    • Alpha 1 globin antibody
    • Alpha globin antibody
    • Alpha one globin antibody
    • Alpha-globin antibody
    • HBA_HUMAN antibody
    • HBA1 antibody
    • HBA2 antibody
    • Hemoglobin alpha 1 antibody
    • Hemoglobin alpha 1 chain antibody
    • Hemoglobin alpha 1 globin chain antibody
    • Hemoglobin alpha 2 antibody
    • Hemoglobin alpha chain antibody
    • Hemoglobin subunit alpha antibody
    • MGC126895 antibody
    • MGC126897 antibody
    see all

Images

  • This IHC data was generated using the same anti-hemoglobin subunit alpha antibody clone, EPR3608, in a different buffer formulation (cat# ab924920).

    Immunohistochemical analysis of paraffin embedded human stomach cancer tissue labeling hemoglobin subunit alpha with unpurified ab92492 at 0.051 µg/ml (1:40000 dilution).

  • This IHC data was generated using the same anti-hemoglobin subunit alpha antibody clone, EPR3608, in a different buffer formulation (cat# ab924920).

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human placenta tissue labeling Hemoglobin subunit alpha antibody with purified ab92492 at 1/500 dilution (1.4 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, PH9. ab97051, a HRP-conjugated goat anti-rabbit IgG H&L was used as the secondary antibody at 1/500 dilution. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

References

ab174536 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab174536.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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