Recombinant
RabMAb

Recombinant Anti-Hemoglobin subunit alpha antibody [SP212] (ab227671)

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Overview

  • Product name

    Anti-Hemoglobin subunit alpha antibody [SP212]
    See all Hemoglobin subunit alpha primary antibodies

  • Description

    Rabbit monoclonal [SP212] to Hemoglobin subunit alpha

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WBmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human Hemoglobin subunit alpha aa 1-100. The exact sequence is proprietary.
    Database link: P69905

  • Positive control

    • WB: Human heart tissue lysate. IHC-P: Human placenta, spleen and skeletal muscle tissue.

  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.1% Sodium azide
    Constituents: 1% BSA, PBS
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    SP212

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab227671 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100.

Antigen retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 minutes followed by cooling at room temperature for 20 minutes.
WB 1/400. Predicted molecular weight: 15 kDa.

Target

  • Function

    Involved in oxygen transport from the lung to the various peripheral tissues.

  • Tissue specificity

    Red blood cells.

  • Involvement in disease

    Heinz body anemias
    Alpha-thalassemia
    Alpha(0)-thalassemia is associated with non-immune hydrops fetalis, a generalized edema of the fetus with fluid accumulation in the body cavities due to non-immune causes. Non-immune hydrops fetalis is not a diagnosis in itself but a symptom, a feature of many genetic disorders, and the end-stage of a wide variety of disorders.
    Hemoglobin H disease

  • Sequence similarities

    Belongs to the globin family.

  • Post-translational
    modifications

    The initiator Met is not cleaved in variant Thionville and is acetylated.
  • Information by UniProt

  • Database links

    • Alternative names

      • Alpha 1 globin antibody
      • Alpha globin antibody
      • Alpha one globin antibody
      • Alpha-globin antibody
      • HBA_HUMAN antibody
      • HBA1 antibody
      • HBA2 antibody
      • Hemoglobin alpha 1 antibody
      • Hemoglobin alpha 1 chain antibody
      • Hemoglobin alpha 1 globin chain antibody
      • Hemoglobin alpha 2 antibody
      • Hemoglobin alpha chain antibody
      • Hemoglobin subunit alpha antibody
      • MGC126895 antibody
      • MGC126897 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hemoglobin subunit alpha antibody [SP212] (ab227671)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hemoglobin subunit alpha antibody [SP212] (ab227671)

      Formalin-fixed paraffin-embedded human spleen tissue stained for Hemoglobin subunit alpha with ab227671 at 1/100 dilution in immunohistochemical analysis.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hemoglobin subunit alpha antibody [SP212] (ab227671)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hemoglobin subunit alpha antibody [SP212] (ab227671)

      Formalin-fixed paraffin-embedded human placenta tissue stained for Hemoglobin subunit alpha with ab227671 at 1/100 dilution in immunohistochemical analysis.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hemoglobin subunit alpha antibody [SP212] (ab227671)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hemoglobin subunit alpha antibody [SP212] (ab227671)

      Formalin-fixed paraffin-embedded human skeletal muscle tissue stained for Hemoglobin subunit alpha with ab227671 at 1/100 dilution in immunohistochemical analysis.

    • Western blot - Anti-Hemoglobin subunit alpha antibody [SP212] (ab227671)
      Western blot - Anti-Hemoglobin subunit alpha antibody [SP212] (ab227671)
      Anti-Hemoglobin subunit alpha antibody [SP212] (ab227671) at 1/400 dilution + Human heart tissue lysate.

      Developed using the ECL technique.

      Predicted band size: 15 kDa

    References

    ab227671 has not yet been referenced specifically in any publications.

    Publishing research using ab227671? Please let us know so that we can cite the reference in this datasheet.

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