Anti-Hemoglobin subunit beta antibody (ab227552)
Key features and details
- Rabbit polyclonal to Hemoglobin subunit beta
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Hemoglobin subunit beta antibody
See all Hemoglobin subunit beta primary antibodies -
Description
Rabbit polyclonal to Hemoglobin subunit beta -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Rhesus monkey -
Immunogen
Recombinant full length protein corresponding to Human Hemoglobin subunit beta.
Database link: P68871 -
Positive control
- WB: Rat spleen extract; Human plasma extract; Untreated and Complete Freunds adjuvant treated mouse leukocyte extracts.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab227552 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 16 kDa.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 16 kDa. |
Target
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Function
Involved in oxygen transport from the lung to the various peripheral tissues.
LVV-hemorphin-7 potentiates the activity of bradykinin, causing a decrease in blood pressure.
Spinorphin: functions as an endogenous inhibitor of enkephalin-degrading enzymes such as DPP3, and as a selective antagonist of the P2RX3 receptor which is involved in pain signaling, these properties implicate it as a regulator of pain and inflammation. -
Tissue specificity
Red blood cells. -
Involvement in disease
Heinz body anemias
Beta-thalassemia
Sickle cell anemia
Beta-thalassemia, dominant, inclusion body type -
Sequence similarities
Belongs to the globin family. -
Post-translational
modificationsGlucose reacts non-enzymatically with the N-terminus of the beta chain to form a stable ketoamine linkage. This takes place slowly and continuously throughout the 120-day life span of the red blood cell. The rate of glycation is increased in patients with diabetes mellitus.
S-nitrosylated; a nitric oxide group is first bound to Fe(2+) and then transferred to Cys-94 to allow capture of O(2).
Acetylated on Lys-60, Lys-83 and Lys-145 upon aspirin exposure. - Information by UniProt
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Database links
- Entrez Gene: 3043 Human
- Entrez Gene: 15127 Mouse
- Entrez Gene: 24440 Rat
- Omim: 141900 Human
- SwissProt: P68871 Human
- Unigene: 523443 Human
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Alternative names
- Beta-globin antibody
- HBB antibody
- HBB_HUMAN antibody
see all
Images
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Anti-Hemoglobin subunit beta antibody (ab227552) at 1/500 dilution + Rat spleen extract at 50 µg
Predicted band size: 16 kDa15% SDS-PAGE gel.
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Anti-Hemoglobin subunit beta antibody (ab227552) at 1/500 dilution + Human plasma extract at 30 µg
Predicted band size: 16 kDa15% SDS-PAGE gel.
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All lanes : Anti-Hemoglobin subunit beta antibody (ab227552) at 1/500 dilution
Lane 1 : Untreated mouse leukocyte extract
Lane 2 : Complete Freunds adjuvant treated mouse leukocyte extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 16 kDa15% SDS-PAGE gel.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab227552 has not yet been referenced specifically in any publications.