Recombinant Anti-Hemoglobin subunit beta antibody [EPR20614] (ab214049)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20614] to Hemoglobin subunit beta
- Suitable for: WB, IP
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
-
Product name
Anti-Hemoglobin subunit beta antibody [EPR20614]
See all Hemoglobin subunit beta primary antibodies -
Description
Rabbit monoclonal [EPR20614] to Hemoglobin subunit beta -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- WB: TF-1 cell lysate; Human lung and fetal heart lysates; Rat heart lysate; Mouse heart and lung lysates. IP: TF-1 cell lysate.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20614 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
-
Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab214049 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/1000. Detects a band of approximately 13 kDa (predicted molecular weight: 15 kDa).
|
|
IP |
1/40.
|
Notes |
---|
WB
1/1000. Detects a band of approximately 13 kDa (predicted molecular weight: 15 kDa). |
IP
1/40. |
Target
-
Function
Involved in oxygen transport from the lung to the various peripheral tissues.
LVV-hemorphin-7 potentiates the activity of bradykinin, causing a decrease in blood pressure.
Spinorphin: functions as an endogenous inhibitor of enkephalin-degrading enzymes such as DPP3, and as a selective antagonist of the P2RX3 receptor which is involved in pain signaling, these properties implicate it as a regulator of pain and inflammation. -
Tissue specificity
Red blood cells. -
Involvement in disease
Heinz body anemias
Beta-thalassemia
Sickle cell anemia
Beta-thalassemia, dominant, inclusion body type -
Sequence similarities
Belongs to the globin family. -
Post-translational
modificationsGlucose reacts non-enzymatically with the N-terminus of the beta chain to form a stable ketoamine linkage. This takes place slowly and continuously throughout the 120-day life span of the red blood cell. The rate of glycation is increased in patients with diabetes mellitus.
S-nitrosylated; a nitric oxide group is first bound to Fe(2+) and then transferred to Cys-94 to allow capture of O(2).
Acetylated on Lys-60, Lys-83 and Lys-145 upon aspirin exposure. - Information by UniProt
-
Database links
- Entrez Gene: 3043 Human
- Entrez Gene: 15127 Mouse
- Entrez Gene: 24440 Rat
- Omim: 141900 Human
- SwissProt: P68871 Human
- Unigene: 523443 Human
-
Alternative names
- Beta-globin antibody
- HBB antibody
- HBB_HUMAN antibody
see all
Images
-
All lanes : Anti-Hemoglobin subunit beta antibody [EPR20614] (ab214049) at 1/1000 dilution
Lane 1 :Recombinant Human Hemoglobin subunit alpha protein (denatured) (ab131697)
Lane 2 : His-tagged human Hemoglobin subunit beta/ba1 (aa3-147) recombinant protein
Lane 3 : His-tagged human Hemoglobin subunit delta (aa3-147) recombinant protein
Lane 4 : His-tagged human Hemoglobin subunit epsilon (aa3-147) recombinant protein
Lane 5 : His-tagged human Hemoglobin subunit Gamma-2 (aa2-147) recombinant protein
Lane 6 : His-tagged human Hemoglobin subunit Gamma-1 (aa2-147) recombinant protein
Lane 7 :Recombinant Human Hemoglobin subunit zeta protein (ab95347)
Lysates/proteins at 0.01 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Developed using the ECL technique.
Predicted band size: 15 kDa
Observed band size: 15 kDa
Exposure time: 3 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
-
Hemoglobin subunit beta was immunoprecipitated from 0.35 mg of TF-1 (human bone marrow erythroleukemia cell line) lysate with ab214049 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab214049 at 1/1,000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution
Lane 1: TF-1 whole cell lysate 10 µg (Input).
Lane 2: ab214049 IP in TF-1 whole cell lysate (+).
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab214049 in TF-1 whole cell lysate (-).
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes.
-
All lanes : Anti-Hemoglobin subunit beta antibody [EPR20614] (ab214049) at 1/1000 dilution
Lane 1 : TF-1 (human bone marrow erythroleukemia cell line) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 15 kDa
Observed band size: 13 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
Positive Control: Anti-GAPDH antibody ab181602 (below)
It has been shown that hemoglobin subunit beta/ba1 is not expressed in HEK-293 cells.
-
All lanes : Anti-Hemoglobin subunit beta antibody [EPR20614] (ab214049) at 1/10000 dilution
Lane 1 : Human fetal heart lysate at 10 µg
Lane 2 : Human lung lysate at 20 µg
Secondary
All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/4000 dilution
Developed using the ECL technique.
Predicted band size: 15 kDa
Observed band size: 13 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
-
Lanes 1 & 3 : Anti-Hemoglobin subunit beta antibody [EPR20614] (ab214049) at 1/1000 dilution
Lane 2 : Anti-Hemoglobin subunit beta antibody [EPR20614] (ab214049) at 1/10000 dilution
Lane 1 : Rat heart lysate at 20 µg
Lane 2 : Mouse heart lysate at 20 µg
Lane 3 : Mouse lung lysate at 10 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 15 kDa
Observed band size: 13 kDa why is the actual band size different from the predicted?Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure times: Lane 1-2: 3 minutes; Lane 3: 10 seconds.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
Certificate of Compliance
References (3)
ab214049 has been referenced in 3 publications.
- Jin T et al. Transmembrane Protein ANTXR1 Regulates γ-Globin Expression by Targeting the Wnt/β-Catenin Signaling Pathway. J Immunol Res 2022:8440422 (2022). PubMed: 35942209
- Zhu G et al. N-Myristoylation by NMT1 Is POTEE-Dependent to Stimulate Liver Tumorigenesis via Differentially Regulating Ubiquitination of Targets. Front Oncol 11:681366 (2021). PubMed: 34136404
- Yang P et al. Supramolecular nanosubstrate-mediated delivery system enables CRISPR-Cas9 knockin of hemoglobin beta gene for hemoglobinopathies. Sci Adv 6:N/A (2020). PubMed: 33097539