Recombinant
RabMAb

Recombinant Anti-Hemoglobin subunit beta/ba1 antibody [EPR20614] (ab214049)

Overview

  • Product name

    Anti-Hemoglobin subunit beta/ba1 antibody [EPR20614]
    See all Hemoglobin subunit beta/ba1 primary antibodies
  • Description

    Rabbit monoclonal [EPR20614] to Hemoglobin subunit beta/ba1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant full length protein within Human Hemoglobin subunit beta/ba1 aa 1 to the C-terminus. The exact sequence is proprietary.
    Database link: P68871

  • Positive control

    • WB: TF-1 cell lysate; Human lung and fetal heart lysates; Rat heart lysate; Mouse heart and lung lysates. IP: TF-1 cell lysate.
  • General notes

     This product was previously labelled as Hemoglobin subunit beta

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 0.05% BSA, 40% Glycerol, PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR20614
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab214049 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 13 kDa (predicted molecular weight: 15 kDa).
IP 1/40.

Target

  • Relevance

    The alpha and beta loci determine the structure of the 2 types of polypeptide chains in the adult hemoglobin, Hb A. The normal adult hemoglobin tetramer consists of two alpha chains and two beta chains. Absence of beta chain causes beta-zero-thalassemia whereas mutant beta globlin causes sickle cell anemia. Reduced amounts of detectable beta globin causes beta-plus-thalassemia.
  • Cellular localization

    Haemoglobin complex, red blood cells.
  • Database links

  • Alternative names

    • ba1 antibody
    • Beta 1 globin antibody
    • beta globin antibody
    • beta globin chain antibody
    • BETA GLOBIN TYPE antibody
    • BETA THALASSEMIAS antibody
    • Beta-A1-globin antibody
    • CD113t C antibody
    • CD113t-C antibody
    • ERYTHREMIA antibody
    • HBB antibody
    • Hemoglobin beta 1 chain antibody
    • hemoglobin beta chain antibody
    • hemoglobin beta chain complex antibody
    • Hemoglobin beta chain, major form antibody
    • HEMOGLOBIN BETA LOCUS antibody
    • Hemoglobin beta-1 chain antibody
    • Hemoglobin subunit beta-1 antibody
    • METHEMOGLOBINEMIA antibody
    see all

Images

  • All lanes : Anti-Hemoglobin subunit beta/ba1 antibody [EPR20614] (ab214049) at 1/10000 dilution

    Lane 1 : His-tagged human hemoglobin subunit beta/ba1 (aa1-147) recombinant protein
    Lane 2 : His-tagged human hemoglobin subunit delta (aa2-147) recombinant protein

    Lysates/proteins at 0.01 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 15 kDa
    Observed band size: 13 kDa
    why is the actual band size different from the predicted?


    Exposure time: 10 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Hemoglobin subunit beta/ba1 was immunoprecipitated from 0.35 mg of TF-1 (human bone marrow erythroleukemia cell line) lysate with ab214049 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab214049 at 1/1,000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution

    Lane 1: TF-1 whole cell lysate 10 µg (Input). 

    Lane 2: ab214049 IP in TF-1 whole cell lysate (+). 

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab214049 in TF-1 whole cell lysate (-).

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes.

  • All lanes : Anti-Hemoglobin subunit beta/ba1 antibody [EPR20614] (ab214049) at 1/1000 dilution

    Lane 1 : His-tagged human hemoglobin subunit epsilon (aa1-147) recombinant protein
    Lane 2 : His-tagged human hemoglobin subunit gamma-2 (aa1-147) recombinant protein

    Lysates/proteins at 0.01 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 15 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • All lanes : Anti-Hemoglobin subunit beta/ba1 antibody [EPR20614] (ab214049) at 1/1000 dilution

    Lane 1 : TF-1 (human bone marrow erythroleukemia cell line) whole cell lysate
    Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 15 kDa
    Observed band size: 13 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

    It has been shown that hemoglobin subunit beta/ba1 is not expressed in HEK-293 cells.

  • All lanes : Anti-Hemoglobin subunit beta/ba1 antibody [EPR20614] (ab214049) at 1/10000 dilution

    Lane 1 : Human fetal heart lysate at 10 µg
    Lane 2 : Human lung lysate at 20 µg

    Secondary
    All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/4000 dilution

    Developed using the ECL technique.

    Predicted band size: 15 kDa
    Observed band size: 13 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Lanes 1 & 3 : Anti-Hemoglobin subunit beta/ba1 antibody [EPR20614] (ab214049) at 1/1000 dilution
    Lane 2 : Anti-Hemoglobin subunit beta/ba1 antibody [EPR20614] (ab214049) at 1/10000 dilution

    Lane 1 : Rat heart lysate at 20 µg
    Lane 2 : Mouse heart lysate at 20 µg
    Lane 3 : Mouse lung lysate at 10 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 15 kDa
    Observed band size: 13 kDa why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure times: Lane 1-2: 3 minutes; Lane 3: 10 seconds.

References

ab214049 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab214049.
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