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RecombinantRabMAb

Recombinant Anti-Hemoglobin subunit delta antibody [EPR8323(B)] - BSA and Azide free (ab248234)

  • Datasheet
  • Certificate of Compliance
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Western blot - Anti-Hemoglobin subunit delta antibody [EPR8323(B)] - BSA and Azide free (ab248234)
  • OI-RD Scanning - Anti-Hemoglobin subunit delta antibody [EPR8323(B)] - BSA and Azide free (ab248234)
  • Anti-Hemoglobin subunit delta antibody [EPR8323(B)] - BSA and Azide free (ab248234)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8323(B)] to Hemoglobin subunit delta - BSA and Azide free
  • Suitable for: WB
  • Reacts with: Human

You may also be interested in

Conjugation
Product image
HRP Conjugation Kit - Lightning-Link® (ab102890)

View more associated products

Overview

  • Product name

    Anti-Hemoglobin subunit delta antibody [EPR8323(B)] - BSA and Azide free
    See all Hemoglobin subunit delta primary antibodies
  • Description

    Rabbit monoclonal [EPR8323(B)] to Hemoglobin subunit delta - BSA and Azide free
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab248234 is the carrier-free version of ab128940 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab248234 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Dissociation constant (KD)

    KD = 1.50 x 10 -12 M
    Learn more about KD
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Affinity purified
  • Clonality

    Monoclonal
  • Clone number

    EPR8323(B)
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Blood
    • Blood Cell Antigens
    • RBC Antigens
    • Other
    • Cardiovascular
    • Blood
    • Erythrocytes

Associated products

  • Alternative Versions

    • Anti-Hemoglobin subunit delta antibody [EPR8323(B)] (ab128940)
  • Conjugation kits

    • HRP Conjugation Kit - Lightning-Link® (ab102890)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control - BSA and Azide Free (ab210849)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab248234 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
WB
Human
Application Abreviews Notes
WB
Use at an assay dependent concentration. Predicted molecular weight: 16 kDa.
Notes
WB
Use at an assay dependent concentration. Predicted molecular weight: 16 kDa.
  • Application notes
    Is unsuitable for IHC-P or IP.
  • Target

    • Function

      Involved in oxygen transport from the lung to the various peripheral tissues.
    • Tissue specificity

      Red blood cells.
    • Sequence similarities

      Belongs to the globin family.
    • Target information above from: UniProt accession P02042 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 3045 Human
      • Omim: 142000 Human
      • SwissProt: P02042 Human
      • Unigene: 699280 Human
      • Alternative names

        • Delta-globin antibody
        • HBD antibody
        • HBD_HUMAN antibody
        • Hemoglobin delta chain antibody
        • Hemoglobin subunit delta antibody
        see all

      Images

      • Western blot - Anti-Hemoglobin subunit delta antibody [EPR8323(B)] - BSA and Azide free (ab248234)
        Western blot - Anti-Hemoglobin subunit delta antibody [EPR8323(B)] - BSA and Azide free (ab248234)
        All lanes : Anti-Hemoglobin subunit delta antibody [EPR8323(B)] (ab128940) at 1/1000 dilution

        Lane 1 : Human fetal heart tissue lysate
        Lane 2 : Human fetal liver tissue lysate
        Lane 3 : Human red blood cell lysate

        Secondary
        All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

        Predicted band size: 16 kDa



        This data was developed using ab128940, the same antibody clone in a different buffer formulation.

      • OI-RD Scanning - Anti-Hemoglobin subunit delta antibody [EPR8323(B)] - BSA and Azide free (ab248234)
        OI-RD Scanning - Anti-Hemoglobin subunit delta antibody [EPR8323(B)] - BSA and Azide free (ab248234)
        This data was developed using ab128940, the same antibody clone in a different buffer formulation.Equilibrium disassociation constant (KD)
        Learn more about KD

        Click here to learn more about KD
      • Anti-Hemoglobin subunit delta antibody [EPR8323(B)] - BSA and Azide free (ab248234)
        Anti-Hemoglobin subunit delta antibody [EPR8323(B)] - BSA and Azide free (ab248234)

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet

      Certificate of Compliance

      To download a Certificate of Compliance, please enter your Lot number below:

    • References (0)

      Publishing research using ab248234? Please let us know so that we can cite the reference in this datasheet.

      ab248234 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

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